FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313557302> ?p ?o ?g. }

• W4313557302 endingPage "134" @default.
• W4313557302 startingPage "121" @default.
• W4313557302 abstract "The coronavirus SARS-CoV-2 causes the severe disease COVID-19. SARS-CoV-2 infection is initiated by interaction of the viral spike protein and host receptor angiotensin-converting enzyme 2 (ACE2). We report an improved bright and reversible fluorogenic reporter, named SURF (split UnaG-based reversible and fluorogenic protein-protein interaction reporter), that we apply to monitor real-time interactions between spike and ACE2 in living cells. SURF has a large dynamic range with a dark-to-bright fluorescence signal that requires no exogenous cofactors. Utilizing this reporter, we carried out a high-throughput screening of small-molecule libraries. We identified three natural compounds that block replication of SARS-CoV-2 in both Vero cells and human primary nasal and bronchial epithelial cells. Cell biological and biochemical experiments validated all three compounds and showed that they block the early stages of viral infection. Two of the inhibitors, bruceine A and gamabufotalin, were also found to block replication of the Delta and Omicron variants of SARS-CoV-2. Both bruceine A and gamabufotalin exhibited potent antiviral activity in K18-hACE2 and wild-type C57BL6/J mice, as evidenced by reduced viral titres in the lung and brain, and protection from alveolar and peribronchial inflammation in the lung, thereby limiting disease progression. We propose that our fluorescent assay can be applied to identify antiviral compounds with potential as therapeutic treatment for COVID-19 and other respiratory diseases." @default.
• W4313557302 created "2023-01-06" @default.
• W4313557302 creator A5001357909 @default.
• W4313557302 creator A5006463024 @default.
• W4313557302 creator A5008558253 @default.
• W4313557302 creator A5009419179 @default.
• W4313557302 creator A5010599370 @default.
• W4313557302 creator A5013517901 @default.
• W4313557302 creator A5029001545 @default.
• W4313557302 creator A5034522642 @default.
• W4313557302 creator A5034866559 @default.
• W4313557302 creator A5038764292 @default.
• W4313557302 creator A5043061422 @default.
• W4313557302 creator A5045642237 @default.
• W4313557302 creator A5045673774 @default.
• W4313557302 creator A5047802437 @default.
• W4313557302 creator A5071404060 @default.
• W4313557302 creator A5073608094 @default.
• W4313557302 creator A5077396845 @default.
• W4313557302 creator A5077529500 @default.
• W4313557302 creator A5080056265 @default.
• W4313557302 creator A5083537639 @default.
• W4313557302 date "2023-01-05" @default.
• W4313557302 modified "2023-10-16" @default.
• W4313557302 title "Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2" @default.
• W4313557302 cites W1979539207 @default.
• W4313557302 cites W1991001144 @default.
• W4313557302 cites W2001589031 @default.
• W4313557302 cites W2014245863 @default.
• W4313557302 cites W2079416470 @default.
• W4313557302 cites W2107913200 @default.
• W4313557302 cites W2115734610 @default.
• W4313557302 cites W2128377922 @default.
• W4313557302 cites W2132465034 @default.
• W4313557302 cites W2336145126 @default.
• W4313557302 cites W3007643904 @default.
• W4313557302 cites W3010441732 @default.
• W4313557302 cites W3011701533 @default.
• W4313557302 cites W3014067025 @default.
• W4313557302 cites W3014656992 @default.
• W4313557302 cites W3015190630 @default.
• W4313557302 cites W3015608194 @default.
• W4313557302 cites W3020300207 @default.
• W4313557302 cites W3022435472 @default.
• W4313557302 cites W3022734398 @default.
• W4313557302 cites W3025226065 @default.
• W4313557302 cites W3040017923 @default.
• W4313557302 cites W3047003416 @default.
• W4313557302 cites W3080378920 @default.
• W4313557302 cites W3084236163 @default.
• W4313557302 cites W3094419073 @default.
• W4313557302 cites W3103254795 @default.
• W4313557302 cites W3104554419 @default.
• W4313557302 cites W3117730334 @default.
• W4313557302 cites W3122927255 @default.
• W4313557302 cites W3134246655 @default.
• W4313557302 cites W3155123963 @default.
• W4313557302 cites W3194798235 @default.
• W4313557302 cites W3196660357 @default.
• W4313557302 cites W3201539868 @default.
• W4313557302 doi "https://doi.org/10.1038/s41564-022-01288-5" @default.
• W4313557302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36604514" @default.
• W4313557302 hasPublicationYear "2023" @default.
• W4313557302 type Work @default.
• W4313557302 citedByCount "5" @default.
• W4313557302 countsByYear W43135573022023 @default.
• W4313557302 crossrefType "journal-article" @default.
• W4313557302 hasAuthorship W4313557302A5001357909 @default.
• W4313557302 hasAuthorship W4313557302A5006463024 @default.
• W4313557302 hasAuthorship W4313557302A5008558253 @default.
• W4313557302 hasAuthorship W4313557302A5009419179 @default.
• W4313557302 hasAuthorship W4313557302A5010599370 @default.
• W4313557302 hasAuthorship W4313557302A5013517901 @default.
• W4313557302 hasAuthorship W4313557302A5029001545 @default.
• W4313557302 hasAuthorship W4313557302A5034522642 @default.
• W4313557302 hasAuthorship W4313557302A5034866559 @default.
• W4313557302 hasAuthorship W4313557302A5038764292 @default.
• W4313557302 hasAuthorship W4313557302A5043061422 @default.
• W4313557302 hasAuthorship W4313557302A5045642237 @default.
• W4313557302 hasAuthorship W4313557302A5045673774 @default.
• W4313557302 hasAuthorship W4313557302A5047802437 @default.
• W4313557302 hasAuthorship W4313557302A5071404060 @default.
• W4313557302 hasAuthorship W4313557302A5073608094 @default.
• W4313557302 hasAuthorship W4313557302A5077396845 @default.
• W4313557302 hasAuthorship W4313557302A5077529500 @default.
• W4313557302 hasAuthorship W4313557302A5080056265 @default.
• W4313557302 hasAuthorship W4313557302A5083537639 @default.
• W4313557302 hasBestOaLocation W43135573021 @default.
• W4313557302 hasConcept C104317684 @default.
• W4313557302 hasConcept C140704245 @default.
• W4313557302 hasConcept C150194340 @default.
• W4313557302 hasConcept C153911025 @default.
• W4313557302 hasConcept C159047783 @default.
• W4313557302 hasConcept C161733203 @default.
• W4313557302 hasConcept C185592680 @default.
• W4313557302 hasConcept C2522874641 @default.
• W4313557302 hasConcept C55493867 @default.
• W4313557302 hasConcept C86803240 @default.

• next