Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313557324> ?p ?o ?g. }
- W4313557324 endingPage "123140" @default.
- W4313557324 startingPage "123140" @default.
- W4313557324 abstract "Organic-inorganic hybrid nanoflowers (hNFs) with commercial protease Neutrase is proposed and characterized as efficient and green biocatalysts for promiscuous catalysis in aldol-type and multicomponent reactions. Neutrase hNFs [Neutrase-(Cu/Ca/Co/Mn)3(PO4)2] are straightforwardly prepared through mixing metal ion (Cu2+, Ca2+, Co2+ or Mn2+) aqueous solutions with Neutrase in phosphate buffer (pH 7.4, 10 mM) resulting in precipitation (3 days). The hNFs were characterized by various techniques including scanning electron microscopy (SEM), energy dispersive X-ray (EDX), element mapping, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). In SEM images, the metal-Neutrase complexes revealed flower-like or granular structures after hybridization. The effect of metal ions and enzyme concentrations on the morphology and enzyme activity of the Neutrase-hNFs was examined. The synthesized Neutrase-Mn hNFs showed superior activity and stability compared to free Neutrase. Traditional organic CC coupling reactions such as aldol condensation, decarboxylative aldol, Knoevenagel, Hantzsch-type reactions and synthesis of 4H-pyran derivatives were used to test the generality and scope of Neutrase promiscuity, while optimizing conditions for the Neutrase-Mn hNF biocatalyst. Briefly, Neutrase-Mn3(PO4)2 hNFs showed excellent enzyme activity, stability and reusability, qualifying as effective reusable catalysts for coupling reactions under mild conditions." @default.
- W4313557324 created "2023-01-06" @default.
- W4313557324 creator A5025137379 @default.
- W4313557324 creator A5036777986 @default.
- W4313557324 creator A5056004565 @default.
- W4313557324 creator A5064250539 @default.
- W4313557324 creator A5072025280 @default.
- W4313557324 creator A5074517880 @default.
- W4313557324 date "2023-03-01" @default.
- W4313557324 modified "2023-10-10" @default.
- W4313557324 title "Immobilization of Bacillus amyloliquefaciens protease “Neutrase” as hybrid enzyme inorganic nanoflower particles: A new biocatalyst for aldol-type and multicomponent reactions" @default.
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- W4313557324 doi "https://doi.org/10.1016/j.ijbiomac.2023.123140" @default.