Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313559377> ?p ?o ?g. }
- W4313559377 endingPage "988" @default.
- W4313559377 startingPage "988" @default.
- W4313559377 abstract "Botrytis cinerea, the causal agent of gray mold, is one of the most destructive pathogens of cherry tomatoes, causing fruit decay and economic loss. Fludioxonil is an effective fungicide widely used for crop protection and is effective against tomato gray mold. The emergence of fungicide-resistant strains has made the control of B. cinerea more difficult. While the genome of B. cinerea is available, there are few reports regarding the large-scale functional annotation of the genome using expressed genes derived from transcriptomes, and the mechanism(s) underlying such fludioxonil resistance remain unclear. The present study prepared RNA-sequencing (RNA-seq) libraries for three B. cinerea strains (two highly resistant (LR and FR) versus one highly sensitive (S) to fludioxonil), with and without fludioxonil treatment, to identify fludioxonil responsive genes that associated to fungicide resistance. Functional enrichment analysis identified nine resistance related DEGs in the fludioxonil-induced LR and FR transcriptome that were simultaneously up-regulated, and seven resistance related DEGs down-regulated. These included adenosine triphosphate (ATP)-binding cassette (ABC) transporter-encoding genes, major facilitator superfamily (MFS) transporter-encoding genes, and the high-osmolarity glycerol (HOG) pathway homologues or related genes. The expression patterns of twelve out of the sixteen fludioxonil-responsive genes, obtained from the RNA-sequence data sets, were validated using quantitative real-time PCR (qRT-PCR). Based on RNA-sequence analysis, it was found that hybrid histidine kinase, fungal HHKs, such as BOS1, BcHHK2, and BcHHK17, probably involved in the fludioxonil resistance of B. cinerea, in addition, a number of ABC and MFS transporter genes that were not reported before, such as BcATRO, BMR1, BMR3, BcNMT1, BcAMF1, BcTOP1, BcVBA2, and BcYHK8, were differentially expressed in the fludioxonil-resistant strains, indicating that overexpression of these efflux transporters located in the plasma membranes may associate with the fludioxonil resistance mechanism of B. cinerea. All together, these lines of evidence allowed us to draw a general portrait of the anti-fludioxonil mechanisms for B. cinerea, and the assembled and annotated transcriptome data provide valuable genomic resources for further study of the molecular mechanisms of B. cinerea resistance to fludioxonil." @default.
- W4313559377 created "2023-01-06" @default.
- W4313559377 creator A5000500193 @default.
- W4313559377 creator A5019112889 @default.
- W4313559377 creator A5022920979 @default.
- W4313559377 creator A5029871612 @default.
- W4313559377 creator A5031818075 @default.
- W4313559377 creator A5056125016 @default.
- W4313559377 creator A5063377124 @default.
- W4313559377 creator A5076699095 @default.
- W4313559377 creator A5090366405 @default.
- W4313559377 date "2023-01-04" @default.
- W4313559377 modified "2023-09-30" @default.
- W4313559377 title "Transcriptomic Analysis of Resistant and Wild-Type Botrytis cinerea Isolates Revealed Fludioxonil-Resistance Mechanisms" @default.
- W4313559377 cites W1765028663 @default.
- W4313559377 cites W1828529897 @default.
- W4313559377 cites W1970896723 @default.
- W4313559377 cites W1971417891 @default.
- W4313559377 cites W1980162952 @default.
- W4313559377 cites W1981349806 @default.
- W4313559377 cites W1981800177 @default.
- W4313559377 cites W1982045900 @default.
- W4313559377 cites W1989742737 @default.
- W4313559377 cites W1994349762 @default.
- W4313559377 cites W2000168831 @default.
- W4313559377 cites W2004936446 @default.
- W4313559377 cites W2011911789 @default.
- W4313559377 cites W2012790625 @default.
- W4313559377 cites W2012866912 @default.
- W4313559377 cites W2014434549 @default.
- W4313559377 cites W2015532251 @default.
- W4313559377 cites W2017795541 @default.
- W4313559377 cites W2022525179 @default.
- W4313559377 cites W2024777389 @default.
- W4313559377 cites W2025076204 @default.
- W4313559377 cites W2027847170 @default.
- W4313559377 cites W2030721380 @default.
- W4313559377 cites W2032793307 @default.
- W4313559377 cites W2033110633 @default.
- W4313559377 cites W2037058666 @default.
- W4313559377 cites W2040734112 @default.
- W4313559377 cites W2046854003 @default.
- W4313559377 cites W2047036395 @default.
- W4313559377 cites W2059488411 @default.
- W4313559377 cites W2060754267 @default.
- W4313559377 cites W2067394862 @default.
- W4313559377 cites W2074850976 @default.
- W4313559377 cites W2075981715 @default.
- W4313559377 cites W2077895909 @default.
- W4313559377 cites W2079588007 @default.
- W4313559377 cites W2082880990 @default.
- W4313559377 cites W2084147498 @default.
- W4313559377 cites W2088551831 @default.
- W4313559377 cites W2091161477 @default.
- W4313559377 cites W2091625394 @default.
- W4313559377 cites W2092221510 @default.
- W4313559377 cites W2094844935 @default.
- W4313559377 cites W2096307897 @default.
- W4313559377 cites W2098117830 @default.
- W4313559377 cites W2098927413 @default.
- W4313559377 cites W2101406878 @default.
- W4313559377 cites W2106329785 @default.
- W4313559377 cites W2107277218 @default.
- W4313559377 cites W2107976600 @default.
- W4313559377 cites W2109079211 @default.
- W4313559377 cites W2118013428 @default.
- W4313559377 cites W2118751052 @default.
- W4313559377 cites W2121211805 @default.
- W4313559377 cites W2122408904 @default.
- W4313559377 cites W2122882986 @default.
- W4313559377 cites W2139456772 @default.
- W4313559377 cites W2142008635 @default.
- W4313559377 cites W2142055985 @default.
- W4313559377 cites W2153562609 @default.
- W4313559377 cites W2155749802 @default.
- W4313559377 cites W2165102314 @default.
- W4313559377 cites W2192080449 @default.
- W4313559377 cites W2296407423 @default.
- W4313559377 cites W2300197643 @default.
- W4313559377 cites W2503368536 @default.
- W4313559377 cites W2522941598 @default.
- W4313559377 cites W2560894474 @default.
- W4313559377 cites W2561444988 @default.
- W4313559377 cites W2618044472 @default.
- W4313559377 cites W2794431184 @default.
- W4313559377 cites W2795400332 @default.
- W4313559377 cites W2809225958 @default.
- W4313559377 cites W2886860088 @default.
- W4313559377 cites W2904758986 @default.
- W4313559377 cites W2904855220 @default.
- W4313559377 cites W2914996391 @default.
- W4313559377 cites W2980560391 @default.
- W4313559377 cites W2981511225 @default.
- W4313559377 cites W3090577745 @default.
- W4313559377 cites W3094875233 @default.
- W4313559377 cites W3128740487 @default.
- W4313559377 doi "https://doi.org/10.3390/ijms24020988" @default.
- W4313559377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36674501" @default.