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- W4313567984 abstract "Metabolic derangement is a key culprit in kidney pathophysiology. Organoids have emerged as a promising in vitro tool for kidney research. Here, we present a fine-tuned protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell (iPSC)-derived kidney organoids using Seahorse XF96. We describe the generation of self-organized three-dimensional kidney organoids, followed by preparation of organoids for Seahorse XF96 analysis. We then detail how to carry out stress tests to determine mitochondrial and glycolytic rates in single kidney organoids." @default.
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- W4313567984 date "2023-03-01" @default.
- W4313567984 modified "2023-10-12" @default.
- W4313567984 title "Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96" @default.
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- W4313567984 doi "https://doi.org/10.1016/j.xpro.2022.101999" @default.
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