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• W4313588639 abstract "Abstract The objective was to study the distal colon myenteric plexus and enteric glial cells (EGCs) in P2X7 receptor-deficient (P2X7-/-) animals after experimental ulcerative colitis on. 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) was injected into distal colon of C57BL/6 (WT) and P2X7 receptor gene-deficient (P2X7-/-, KO) animals. Distal colon tissues were analysed 24 h and 4 d after administration. Double immunofluorescence was used for analyses and histology was used for morphological analysis. Quantitative analysis demonstrated 13.9% and 7.1% decreases in the number/ganglia of P2X7 receptor-immunoreactive (ir) in the 24 h-WT/colitis group and 4 d-WT/colitis, respectively. There was no reduction in the number per ganglia of neuronal nitric oxide synthase (nNOS)-ir, choline acetyltransferase (ChAT)-ir and PGP9.5 (pan neuronal)-ir neurons in the 4 d-KO/colitis group. There was a reduction by 19.3% in the number of glial fibrillary acidic protein (GFAP, EGC)-ir in the 24 h-WT/colitis group and a 19% increase in the number of these cells in the 4 d-WT/colitis group. There were no profile area changes in neurons in the 24 h groups. In the 4 d-WT/colitis and 4 d-KO/colitis groups, there was an increase in the profile neuronal area of nNOS, ChAT and PGP9.5. Histological analysis showed hyperaemia, oedema or cellular infiltration in the 24 h-WT/colitis groups and 4 d-WT/colitis groups. The 4 d-KO/colitis groups showed no histological changes." @default.
• W4313588639 created "2023-01-06" @default.
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• W4313588639 date "2023-01-04" @default.
• W4313588639 modified "2023-10-16" @default.
• W4313588639 title "Effects of experimental ulcerative colitis on the myenteric neurons in the P2X7 knockout mice" @default.
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• W4313588639 doi "https://doi.org/10.21203/rs.3.rs-2426530/v1" @default.
• W4313588639 hasPublicationYear "2023" @default.
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