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- W4313592811 abstract "As a raw material for chocolate making process, cacao pods and beans are vulnerable to phytopathogenic fungal attacks. The use of antifungal compounds for controlling the severity degree of such disease is foreseen to be a sustainable alternative. Antifungal activity in cacao seed coat slime has been reported previously, and we expect that microbes associated with the seed coat also produce antifungal compounds. In this study, we isolated bacterial strains associated with the seed coat slime from healthy and unhealthy cacao pods collected from different regions in Indonesia. Based on 16S rRNA gene sequence analysis, we identified one promising strain named Bacillus subtilis IDLOMBOK-02. The strain exhibited a broad inhibitory activity against yeast species that are ecologically relevant in cacao plants, a fungal species Trichoderma sp., and a clinically relevant pathogenic yeast Candida albicans. Batch fermentation of the potential strain for lab-scale antifungal protein production showed that the total protein produced was 3.710 g/L. However, a living cell suspension was more effective in inhibiting pathogenic yeast and fungi than the cell-free supernatant. This study expands a plethora of antifungal proteins from cacao-associated bacteria, which can be harvested and optimized for antifungal metabolites production." @default.
- W4313592811 created "2023-01-06" @default.
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- W4313592811 date "2023-01-01" @default.
- W4313592811 modified "2023-09-30" @default.
- W4313592811 title "Exploration of antifungal activity from cacao seed coat slime-associated bacteria" @default.
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- W4313592811 doi "https://doi.org/10.1063/5.0119481" @default.
- W4313592811 hasPublicationYear "2023" @default.
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