Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313593655> ?p ?o ?g. }
- W4313593655 endingPage "122264" @default.
- W4313593655 startingPage "122264" @default.
- W4313593655 abstract "Spermidine is an aliphatic polyamine that directs a set of biological processes. This work aimed to use UV-Vis spectroscopy, fluorescence spectroscopy, thermal stability, kinetic methods, docking, and molecular dynamic simulations to examine the influence of spermidine trihydrochloride (SP) on the structure and function of pepsin. The results of the fluorescence emission spectra indicated that spermidine could quench pepsin's intrinsic emission in a static quenching process, resulting in the formation of the pepsin-spermidine complex. The results discovered that spermidine had a strong affinity to the pepsin structure because of its high binding constant. The obtained results from spectroscopy and molecular dynamic approaches showed the binding interaction between spermidine and pepsin, induced micro-environmental modifications around tryptophan residues that caused a change in the tertiary and secondary structure of the enzyme. FTIR analysis showed hypochromic effects in the spectra of amide I and II and redistribution of the helical structure. Moreover, the molecular dynamic (MD) and docking studies confirmed the experimental data. Both experimental and molecular dynamics simulation results clarified that electrostatic bond interactions were dominant forces." @default.
- W4313593655 created "2023-01-06" @default.
- W4313593655 creator A5007457491 @default.
- W4313593655 creator A5052359629 @default.
- W4313593655 creator A5060419630 @default.
- W4313593655 creator A5079008796 @default.
- W4313593655 date "2023-04-01" @default.
- W4313593655 modified "2023-09-30" @default.
- W4313593655 title "Structural change study of pepsin in the presence of spermidine trihydrochloride: Insights from spectroscopic to molecular dynamics methods" @default.
- W4313593655 cites W1576021968 @default.
- W4313593655 cites W1983660868 @default.
- W4313593655 cites W1995373379 @default.
- W4313593655 cites W2018268596 @default.
- W4313593655 cites W2024182439 @default.
- W4313593655 cites W2034549041 @default.
- W4313593655 cites W2036644638 @default.
- W4313593655 cites W2042088368 @default.
- W4313593655 cites W2042419061 @default.
- W4313593655 cites W2049510609 @default.
- W4313593655 cites W2050384029 @default.
- W4313593655 cites W2071548518 @default.
- W4313593655 cites W2075705506 @default.
- W4313593655 cites W2090154084 @default.
- W4313593655 cites W2092804642 @default.
- W4313593655 cites W2098441527 @default.
- W4313593655 cites W2099063059 @default.
- W4313593655 cites W2122891240 @default.
- W4313593655 cites W2126429872 @default.
- W4313593655 cites W2150747976 @default.
- W4313593655 cites W2154625685 @default.
- W4313593655 cites W2155365495 @default.
- W4313593655 cites W2259189936 @default.
- W4313593655 cites W2298710313 @default.
- W4313593655 cites W2346606536 @default.
- W4313593655 cites W2441874336 @default.
- W4313593655 cites W2498794773 @default.
- W4313593655 cites W2507553664 @default.
- W4313593655 cites W2539924915 @default.
- W4313593655 cites W2559115459 @default.
- W4313593655 cites W2598723268 @default.
- W4313593655 cites W2604337791 @default.
- W4313593655 cites W2606537295 @default.
- W4313593655 cites W2614796879 @default.
- W4313593655 cites W2661339346 @default.
- W4313593655 cites W2725135651 @default.
- W4313593655 cites W2739080658 @default.
- W4313593655 cites W2775720995 @default.
- W4313593655 cites W2803438754 @default.
- W4313593655 cites W2886456330 @default.
- W4313593655 cites W2891535956 @default.
- W4313593655 cites W2891978572 @default.
- W4313593655 cites W2895712489 @default.
- W4313593655 cites W2896541023 @default.
- W4313593655 cites W2911804266 @default.
- W4313593655 cites W2921277877 @default.
- W4313593655 cites W2932979002 @default.
- W4313593655 cites W2948280374 @default.
- W4313593655 cites W2956321023 @default.
- W4313593655 cites W2967101935 @default.
- W4313593655 cites W2969762143 @default.
- W4313593655 cites W2995531123 @default.
- W4313593655 cites W2999048899 @default.
- W4313593655 cites W2999979300 @default.
- W4313593655 cites W3000427550 @default.
- W4313593655 cites W3005377059 @default.
- W4313593655 cites W3011827683 @default.
- W4313593655 cites W3023542861 @default.
- W4313593655 cites W3088251078 @default.
- W4313593655 cites W3090863464 @default.
- W4313593655 cites W3092705762 @default.
- W4313593655 cites W3107801542 @default.
- W4313593655 cites W3115373086 @default.
- W4313593655 cites W3121008803 @default.
- W4313593655 cites W3130268493 @default.
- W4313593655 cites W3134364834 @default.
- W4313593655 cites W3135157690 @default.
- W4313593655 cites W3142300944 @default.
- W4313593655 cites W3159750120 @default.
- W4313593655 cites W3163829211 @default.
- W4313593655 cites W3166820337 @default.
- W4313593655 cites W3192302396 @default.
- W4313593655 cites W3196431622 @default.
- W4313593655 cites W3217666177 @default.
- W4313593655 cites W4210439699 @default.
- W4313593655 cites W4251211701 @default.
- W4313593655 cites W4283786986 @default.
- W4313593655 cites W4293577745 @default.
- W4313593655 cites W602643467 @default.
- W4313593655 cites W3160938818 @default.
- W4313593655 doi "https://doi.org/10.1016/j.saa.2022.122264" @default.
- W4313593655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36652806" @default.
- W4313593655 hasPublicationYear "2023" @default.
- W4313593655 type Work @default.
- W4313593655 citedByCount "1" @default.
- W4313593655 countsByYear W43135936552023 @default.
- W4313593655 crossrefType "journal-article" @default.
- W4313593655 hasAuthorship W4313593655A5007457491 @default.
- W4313593655 hasAuthorship W4313593655A5052359629 @default.