Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313593802> ?p ?o ?g. }
- W4313593802 endingPage "122583" @default.
- W4313593802 startingPage "122583" @default.
- W4313593802 abstract "The objective of this proof-of-concept study was to evaluate the synergistic effect of a subunit microparticulate vaccine and microneedles (MN) assisted vaccine delivery system against a human coronavirus. Here, we formulated PLGA polymeric microparticles (MPs) encapsulating spike glycoprotein (GP) of SARS-CoV as the model antigen. Similarly, we formulated adjuvant MPs encapsulating Alhydrogel® and AddaVax™. The antigen/adjuvant MPs were characterized and tested in vitro for immunogenicity. We found that the antigen/adjuvant MPs were non-cytotoxic in vitro. The spike GP MPs + Alhydrogel® MPs + AddaVax™ MPs showed enhanced immunogenicity in vitro as confirmed through the release of nitrite, autophagy, and antigen presenting molecules with their co-stimulatory molecules. Next, we tested the in vivo efficacy of the spike GP MP vaccine with and without adjuvant MPs in mice vaccinated using MN. The spike GP MPs + Alhydrogel® MPs + AddaVax™ MPs induced heightened spike GP-specific IgG, IgG1 and IgG2a antibodies in mice. Also, spike GP MPs + Alhydrogel® MPs + AddaVax™ MPs enhanced expression of CD4+ and CD8+ T cells in secondary lymphoid organ like spleen. These results indicated spike GP-specific humoral immunity and cellular immunity in vivo. Thus, we employed the benefits of both the subunit vaccine MPs and dissolving MN to form a non-invasive and effective vaccination strategy against human coronaviruses." @default.
- W4313593802 created "2023-01-06" @default.
- W4313593802 creator A5001988994 @default.
- W4313593802 creator A5013944892 @default.
- W4313593802 creator A5017680310 @default.
- W4313593802 creator A5022620655 @default.
- W4313593802 creator A5028012167 @default.
- W4313593802 creator A5045983400 @default.
- W4313593802 creator A5050339428 @default.
- W4313593802 creator A5052661085 @default.
- W4313593802 creator A5057952304 @default.
- W4313593802 creator A5074179435 @default.
- W4313593802 date "2023-02-01" @default.
- W4313593802 modified "2023-09-26" @default.
- W4313593802 title "Subunit microparticulate vaccine delivery using microneedles trigger significant SARS-spike-specific humoral and cellular responses in a preclinical murine model" @default.
- W4313593802 cites W1839141223 @default.
- W4313593802 cites W1973196190 @default.
- W4313593802 cites W1974941123 @default.
- W4313593802 cites W1982125899 @default.
- W4313593802 cites W1989870914 @default.
- W4313593802 cites W1992787595 @default.
- W4313593802 cites W2012505332 @default.
- W4313593802 cites W2030951969 @default.
- W4313593802 cites W2039419943 @default.
- W4313593802 cites W2039430806 @default.
- W4313593802 cites W2046413950 @default.
- W4313593802 cites W2047759150 @default.
- W4313593802 cites W2053805279 @default.
- W4313593802 cites W2071521950 @default.
- W4313593802 cites W2095233225 @default.
- W4313593802 cites W2102237050 @default.
- W4313593802 cites W2106183630 @default.
- W4313593802 cites W2171480404 @default.
- W4313593802 cites W2203910881 @default.
- W4313593802 cites W2240262155 @default.
- W4313593802 cites W2536069162 @default.
- W4313593802 cites W25898841 @default.
- W4313593802 cites W2735670013 @default.
- W4313593802 cites W2766014141 @default.
- W4313593802 cites W2884350335 @default.
- W4313593802 cites W3013541100 @default.
- W4313593802 cites W3022701653 @default.
- W4313593802 cites W3025521550 @default.
- W4313593802 cites W3110918569 @default.
- W4313593802 cites W3144140615 @default.
- W4313593802 cites W3154545544 @default.
- W4313593802 cites W3166116212 @default.
- W4313593802 cites W3170342439 @default.
- W4313593802 cites W3186917712 @default.
- W4313593802 cites W3194675236 @default.
- W4313593802 cites W4200558562 @default.
- W4313593802 cites W4211143846 @default.
- W4313593802 cites W4229458272 @default.
- W4313593802 cites W4248366088 @default.
- W4313593802 cites W4282922491 @default.
- W4313593802 doi "https://doi.org/10.1016/j.ijpharm.2023.122583" @default.
- W4313593802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36610521" @default.
- W4313593802 hasPublicationYear "2023" @default.
- W4313593802 type Work @default.
- W4313593802 citedByCount "5" @default.
- W4313593802 countsByYear W43135938022023 @default.
- W4313593802 crossrefType "journal-article" @default.
- W4313593802 hasAuthorship W4313593802A5001988994 @default.
- W4313593802 hasAuthorship W4313593802A5013944892 @default.
- W4313593802 hasAuthorship W4313593802A5017680310 @default.
- W4313593802 hasAuthorship W4313593802A5022620655 @default.
- W4313593802 hasAuthorship W4313593802A5028012167 @default.
- W4313593802 hasAuthorship W4313593802A5045983400 @default.
- W4313593802 hasAuthorship W4313593802A5050339428 @default.
- W4313593802 hasAuthorship W4313593802A5052661085 @default.
- W4313593802 hasAuthorship W4313593802A5057952304 @default.
- W4313593802 hasAuthorship W4313593802A5074179435 @default.
- W4313593802 hasBestOaLocation W43135938022 @default.
- W4313593802 hasConcept C147483822 @default.
- W4313593802 hasConcept C150903083 @default.
- W4313593802 hasConcept C154317977 @default.
- W4313593802 hasConcept C159047783 @default.
- W4313593802 hasConcept C179223381 @default.
- W4313593802 hasConcept C185592680 @default.
- W4313593802 hasConcept C202751555 @default.
- W4313593802 hasConcept C203014093 @default.
- W4313593802 hasConcept C207001950 @default.
- W4313593802 hasConcept C2776548049 @default.
- W4313593802 hasConcept C2777863537 @default.
- W4313593802 hasConcept C2780868878 @default.
- W4313593802 hasConcept C55493867 @default.
- W4313593802 hasConcept C86803240 @default.
- W4313593802 hasConcept C8891405 @default.
- W4313593802 hasConceptScore W4313593802C147483822 @default.
- W4313593802 hasConceptScore W4313593802C150903083 @default.
- W4313593802 hasConceptScore W4313593802C154317977 @default.
- W4313593802 hasConceptScore W4313593802C159047783 @default.
- W4313593802 hasConceptScore W4313593802C179223381 @default.
- W4313593802 hasConceptScore W4313593802C185592680 @default.
- W4313593802 hasConceptScore W4313593802C202751555 @default.
- W4313593802 hasConceptScore W4313593802C203014093 @default.
- W4313593802 hasConceptScore W4313593802C207001950 @default.
- W4313593802 hasConceptScore W4313593802C2776548049 @default.