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- W4313594676 abstract "A hydrophilic adsorbent (Cys@poly(AMA)@MAR) was successfully prepared for the enrichment of N-glycopeptides via surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated thiol-ene reaction using monodisperse macroporous adsorbent resin (MAR) as adsorption matrix. Due to the presence of electron-deficient acrylic groups and electron-rich vinyl groups in allyl methacrylate (AMA), both of them can participate in free radical reaction. Therefore, the polymerization time of SI-ATRP was optimized. The resulting poly(AMA)@MAR was modified with l-cysteine (L-Cys) via photo-initiated thiol-ene reaction, and the amount of vinyl retained was determined by measuring the adsorption of Cu2+. The Cys@poly(AMA)@MAR pendant brushes with high density of amine and carboxyl groups could capture N-glycopeptides from IgG digest and human serum digest by hydrophilic interaction. The 22 N-glycopeptides were identified from IgG digest and the limit of detection reached 10 fmol. The 319 N-glycosylation sites and 583 N-glycopeptides were identified from 2 μL human serum digest and mapped to 147 glycoproteins. It demonstrates great potential and commercialization prospects for the enrichment of N-glycopeptides." @default.
- W4313594676 created "2023-01-06" @default.
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- W4313594676 date "2023-01-01" @default.
- W4313594676 modified "2023-10-15" @default.
- W4313594676 title "A combination of surface-initiated atom transfer radical polymerization and photo-initiated “thiol-ene” click chemistry: Fabrication of functionalized macroporous adsorption resins for enrichment of glycopeptides" @default.
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- W4313594676 doi "https://doi.org/10.1016/j.chroma.2023.463774" @default.
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