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• W4313594802 endingPage "739226" @default.
• W4313594802 startingPage "739226" @default.
• W4313594802 abstract "Artificial rearing techniques of larvae and juveniles of the tropical sea cucumber, Actinopyga lecanora were developed for the first time. Broodstock individuals were collected in Yaeyama Islands, Okinawa, subtropical Japan and sorted by the gonad maturity which was determined by biopsy. An artificially-synthesized relaxin peptide, originated from Holothuria (Metriatyla) scabra, was injected into the coelomic fluid of mature A. lecanora at amounts of 3 × 10−9 to 10 × 10−9 mol kg−1 body weight, which successfully induced spawning in 10 of 14 females and 3 of 3 males after 26–70 min. The number of eggs spawned per one female individual ranged 2.2–9.5 × 105. The mean diameter of oocytes of A. lecanora individuals which spawned by the induction was 324 ± 13 μm, much larger than those of sea cucumber species having a planktotrophic larval stage. The embryo hatched during gastrula, and the larva metamorphosed to planktonic auricularia followed by doliolaria and finally settled into the benthic stage as pentactula. Time after fertilization to settlement was 4–8 days when larvae were reared in 5–500 L tanks at 26–28 °C, which is one of the shortest development periods among sea cucumbers. Although larvae were supplied with Chaetoceros gracilis cells in most rearing batches, the larvae were later confirmed as lecithotrophic (non-feeding) as (1) larvae were successfully reared until settlement without feeding, and (2) chlorophyll a fluorescence of C. gracilis was not incorporated into the larval intestine as observed under fluorescence microscopy. Auricularia larvae showed poor development of lateral arm protrusion and did not form hyaline spheres. Initial rearing densities under 0.4 larva mL−1 resulted in relatively good survival. Settling larvae were retrieved using polycarbonate corrugated sheets on which periphytic diatoms had been grown beforehand. Survival from hatching to 2 months after settlement was 1.0–1.8%, comparable with values reported for other sea cucumbers. Postsettled juveniles became orange-colored with black dorsal papillae in 50 days after settlement and subsequently gradually obtained a dark green-brown dorsal pigment. Juveniles having anal teeth and adult-like color pattern appeared from 4 months after settlement. Growth of the settled juveniles was 91.8 ± 1.5 μm day−1 from Jun to Dec, reaching 15.11 ± 5.63 mm long in 170 days, while the juveniles negatively grew during winter from Dec to Feb. The lecithotrophic short larval period of A. lecanora has potential for low-cost seed production, which is advantageous for development of aquaculture and restocking." @default.
• W4313594802 created "2023-01-06" @default.
• W4313594802 creator A5022677274 @default.
• W4313594802 creator A5055680634 @default.
• W4313594802 creator A5070064607 @default.
• W4313594802 creator A5085418887 @default.
• W4313594802 creator A5089504256 @default.
• W4313594802 date "2023-03-01" @default.
• W4313594802 modified "2023-09-25" @default.
• W4313594802 title "Artificial rearing of Actinopyga lecanora (Holothuroidea: Holothuriida) with spawning induction using relaxin: Lecithotrophic short larval period" @default.
• W4313594802 cites W1489454212 @default.
• W4313594802 cites W1662276001 @default.
• W4313594802 cites W1790256397 @default.
• W4313594802 cites W1924106145 @default.
• W4313594802 cites W1965792614 @default.
• W4313594802 cites W1972386127 @default.
• W4313594802 cites W1978201454 @default.
• W4313594802 cites W1979468621 @default.
• W4313594802 cites W1983445055 @default.
• W4313594802 cites W1985133992 @default.
• W4313594802 cites W1987053378 @default.
• W4313594802 cites W1990407903 @default.
• W4313594802 cites W1991576290 @default.
• W4313594802 cites W2001888122 @default.
• W4313594802 cites W2001903553 @default.
• W4313594802 cites W2006024777 @default.
• W4313594802 cites W2007041146 @default.
• W4313594802 cites W2008897450 @default.
• W4313594802 cites W2011674151 @default.
• W4313594802 cites W2012830422 @default.
• W4313594802 cites W2016903112 @default.
• W4313594802 cites W2028666597 @default.
• W4313594802 cites W2031406214 @default.
• W4313594802 cites W2032862445 @default.
• W4313594802 cites W2033177756 @default.
• W4313594802 cites W2039433007 @default.
• W4313594802 cites W2047050589 @default.
• W4313594802 cites W2055575472 @default.
• W4313594802 cites W2060247658 @default.
• W4313594802 cites W2065918430 @default.
• W4313594802 cites W2066481414 @default.
• W4313594802 cites W2068379914 @default.
• W4313594802 cites W2072276236 @default.
• W4313594802 cites W2073653626 @default.
• W4313594802 cites W2076720979 @default.
• W4313594802 cites W2077440863 @default.
• W4313594802 cites W2085133017 @default.
• W4313594802 cites W2086957878 @default.
• W4313594802 cites W2087140567 @default.
• W4313594802 cites W2093074833 @default.
• W4313594802 cites W2093083214 @default.
• W4313594802 cites W2094318259 @default.
• W4313594802 cites W2096948413 @default.
• W4313594802 cites W2099540110 @default.
• W4313594802 cites W2104862677 @default.
• W4313594802 cites W2111448352 @default.
• W4313594802 cites W2114315310 @default.
• W4313594802 cites W2116544762 @default.
• W4313594802 cites W2119368471 @default.
• W4313594802 cites W2130962996 @default.
• W4313594802 cites W2131344247 @default.
• W4313594802 cites W2132109055 @default.
• W4313594802 cites W2134112474 @default.
• W4313594802 cites W2137548532 @default.
• W4313594802 cites W2140420256 @default.
• W4313594802 cites W2153520166 @default.
• W4313594802 cites W2157995790 @default.
• W4313594802 cites W2159771513 @default.
• W4313594802 cites W2160858330 @default.
• W4313594802 cites W2166258948 @default.
• W4313594802 cites W2168477809 @default.
• W4313594802 cites W2179528641 @default.
• W4313594802 cites W2181420279 @default.
• W4313594802 cites W2327302219 @default.
• W4313594802 cites W2469190808 @default.
• W4313594802 cites W2509374271 @default.
• W4313594802 cites W2519143459 @default.
• W4313594802 cites W2547916243 @default.
• W4313594802 cites W2557690892 @default.
• W4313594802 cites W2626443946 @default.
• W4313594802 cites W2756440817 @default.
• W4313594802 cites W2770338649 @default.
• W4313594802 cites W2782147656 @default.
• W4313594802 cites W2783176077 @default.
• W4313594802 cites W2783523590 @default.
• W4313594802 cites W2793262981 @default.
• W4313594802 cites W2809313832 @default.
• W4313594802 cites W2884914635 @default.
• W4313594802 cites W2888612234 @default.
• W4313594802 cites W2918084289 @default.
• W4313594802 cites W2944947872 @default.
• W4313594802 cites W2950692346 @default.
• W4313594802 cites W2951021924 @default.
• W4313594802 cites W2963433209 @default.
• W4313594802 cites W2991449745 @default.
• W4313594802 cites W2999115176 @default.
• W4313594802 cites W3016864984 @default.
• W4313594802 cites W3033661320 @default.

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