FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313595761> ?p ?o ?g. }

• W4313595761 abstract "Background: We have hypothesized that different factors are involved in the severity of ACD and AD because some but not all patients with atopic dermatitis (AD) present with allergic conjunctival disease (ACD) including severe types such as atopic keratoconjunctivitis (AKC) with/without giant papillae. We previously reported that plasma miR-628-3p was up-regulated in AD with severe ACD, but not in severe AD without severe ACD or in our healthy controls. In this study, to investigate the pathogenesis of AD with and without severe ACD, we performed comprehensive plasma miRNA analysis and studied the function of some miRNAs which were significantly up-regulated in ACD. Methods: Transcriptomics analysis of miRNA was performed using the microarray platform from the plasma of nine individuals (AD, severe ACD, controls: n = 3 each). To confirm up-regulation of the 12 miRNAs of the eight miRNA groups we focused on, we performed quantitative miRNA polymerase chain reaction (PCR) assays using 80 plasma samples (AD: 23, severe ACD: 17, controls: 40). To study the function of the eight miRNAs which were significantly up-regulated in ACD, we transfected their mimic to THP-1 cells, a monocyte cell line, and performed comprehensive gene expression analysis of them. The up-regulation of gene expression of interest in transfected THP-1 cells with the hsa-let-7a-5p miRNA mimic was confirmed by quantitative RT-qPCR assay. Results: Quantitative miRNA PCR assays showed that hsa-let-7a-5p, hsa-let-7days-3p, hsa-let-7e-5p, and hsa-miR-151a-5p were significantly up-regulated in both AD-ACD + and AD-ACD - as were hsa-miR-130a-3p, hsa-miR-151a-3p, has-miR-27b-3p, and hsa-miR-146a-5p in AD-ACD + but not in AD-ACD - . The functions of each miRNA were investigated by comprehensive gene expression analysis of THP-1 cells transfected with each miRNA mimic. Of the eight miRNAs, hsa-let-7a-5p, hsa-let-7e-5p, has-miR-27b-3p, and hsa-miR-146a-5p mimic-transfected THP-1 cells showed the up-regulation of CXCL10 (IP-10; interferon gamma-induced protein 10), which might be one of the innate immune-related genes. Quantitative RT-qPCR assays of transfected THP-1 cells with the hsa-let-7a-5p miRNA mimic showed that the 17 genes up-regulated more than 10-fold in the comprehensive gene expression analysis, and TLR3, RIG-I, and MDA5, important innate immune-related genes, were significantly up-regulated. TNFSF13B, AIM2, USP41, STAP1, GBP4, CCL8, and IFI27, reportedly down-regulated by the hsa-miR-628-3p mimic, were also significantly up-regulated in the transfected cells. Conclusion: Hsa-let-7a-5p, which was significantly up-regulated in AD-ACD + and AD-ACD - , could positively regulate the important innate immune-related genes such as TLR3, RIG-I, and MDA5. It is possible that in an allergic disease such as atopic keratoconjunctivitis and/or dermatitis, innate immune responses might be positively regulated by hsa-let-7a-5p in the plasma." @default.
• W4313595761 created "2023-01-06" @default.
• W4313595761 creator A5000736594 @default.
• W4313595761 creator A5020320910 @default.
• W4313595761 creator A5029687156 @default.
• W4313595761 creator A5030364184 @default.
• W4313595761 creator A5030687841 @default.
• W4313595761 creator A5055620042 @default.
• W4313595761 creator A5061869584 @default.
• W4313595761 creator A5062238503 @default.
• W4313595761 creator A5072779683 @default.
• W4313595761 date "2023-01-06" @default.
• W4313595761 modified "2023-10-14" @default.
• W4313595761 title "Positive regulation of innate immune response by miRNA-let-7a-5p" @default.
• W4313595761 cites W2047516222 @default.
• W4313595761 cites W2084684268 @default.
• W4313595761 cites W2150767213 @default.
• W4313595761 cites W2156535644 @default.
• W4313595761 cites W2403616048 @default.
• W4313595761 cites W2565039466 @default.
• W4313595761 cites W2602016111 @default.
• W4313595761 cites W2743960099 @default.
• W4313595761 cites W2790068409 @default.
• W4313595761 cites W2896682976 @default.
• W4313595761 cites W3024101826 @default.
• W4313595761 cites W3093432178 @default.
• W4313595761 cites W3096893697 @default.
• W4313595761 cites W3165606813 @default.
• W4313595761 cites W3200884609 @default.
• W4313595761 doi "https://doi.org/10.3389/fgene.2022.1025539" @default.
• W4313595761 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36685889" @default.
• W4313595761 hasPublicationYear "2023" @default.
• W4313595761 type Work @default.
• W4313595761 citedByCount "2" @default.
• W4313595761 countsByYear W43135957612023 @default.
• W4313595761 crossrefType "journal-article" @default.
• W4313595761 hasAuthorship W4313595761A5000736594 @default.
• W4313595761 hasAuthorship W4313595761A5020320910 @default.
• W4313595761 hasAuthorship W4313595761A5029687156 @default.
• W4313595761 hasAuthorship W4313595761A5030364184 @default.
• W4313595761 hasAuthorship W4313595761A5030687841 @default.
• W4313595761 hasAuthorship W4313595761A5055620042 @default.
• W4313595761 hasAuthorship W4313595761A5061869584 @default.
• W4313595761 hasAuthorship W4313595761A5062238503 @default.
• W4313595761 hasAuthorship W4313595761A5072779683 @default.
• W4313595761 hasBestOaLocation W43135957611 @default.
• W4313595761 hasConcept C104317684 @default.
• W4313595761 hasConcept C145059251 @default.
• W4313595761 hasConcept C150194340 @default.
• W4313595761 hasConcept C153911025 @default.
• W4313595761 hasConcept C186836561 @default.
• W4313595761 hasConcept C203014093 @default.
• W4313595761 hasConcept C2780942790 @default.
• W4313595761 hasConcept C48023723 @default.
• W4313595761 hasConcept C54009773 @default.
• W4313595761 hasConcept C54355233 @default.
• W4313595761 hasConcept C71924100 @default.
• W4313595761 hasConcept C86803240 @default.
• W4313595761 hasConcept C8891405 @default.
• W4313595761 hasConceptScore W4313595761C104317684 @default.
• W4313595761 hasConceptScore W4313595761C145059251 @default.
• W4313595761 hasConceptScore W4313595761C150194340 @default.
• W4313595761 hasConceptScore W4313595761C153911025 @default.
• W4313595761 hasConceptScore W4313595761C186836561 @default.
• W4313595761 hasConceptScore W4313595761C203014093 @default.
• W4313595761 hasConceptScore W4313595761C2780942790 @default.
• W4313595761 hasConceptScore W4313595761C48023723 @default.
• W4313595761 hasConceptScore W4313595761C54009773 @default.
• W4313595761 hasConceptScore W4313595761C54355233 @default.
• W4313595761 hasConceptScore W4313595761C71924100 @default.
• W4313595761 hasConceptScore W4313595761C86803240 @default.
• W4313595761 hasConceptScore W4313595761C8891405 @default.
• W4313595761 hasLocation W43135957611 @default.
• W4313595761 hasLocation W43135957612 @default.
• W4313595761 hasLocation W43135957613 @default.
• W4313595761 hasOpenAccess W4313595761 @default.
• W4313595761 hasPrimaryLocation W43135957611 @default.
• W4313595761 hasRelatedWork W1980494884 @default.
• W4313595761 hasRelatedWork W1983994322 @default.
• W4313595761 hasRelatedWork W2009966535 @default.
• W4313595761 hasRelatedWork W2069954171 @default.
• W4313595761 hasRelatedWork W2362238002 @default.
• W4313595761 hasRelatedWork W2363311673 @default.
• W4313595761 hasRelatedWork W2383598698 @default.
• W4313595761 hasRelatedWork W2385534162 @default.
• W4313595761 hasRelatedWork W2415025170 @default.
• W4313595761 hasRelatedWork W3032874291 @default.
• W4313595761 hasVolume "13" @default.
• W4313595761 isParatext "false" @default.
• W4313595761 isRetracted "false" @default.
• W4313595761 workType "article" @default.