Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313625062> ?p ?o ?g. }
- W4313625062 abstract "Abstract The K + channel selectivity filter (SF) is defined by TxGYG amino acid sequences that generate four identical K + binding sites (S1-S4). Only two sites (S3, S4) are present in the non-selective bacterial NaK channel, but a four-site K + -selective SF is obtained by mutating the wild-type TVGDGN SF sequence to a canonical K + channel TVGYGD sequence (NaK2K mutant). Using single molecule FRET (smFRET), we show that the SF of NaK2K, but not of non-selective NaK, is ion-dependent, with the constricted SF configuration stabilized in high K + conditions. Patch-clamp electrophysiology and non-canonical fluorescent amino acid incorporation show that NaK2K selectivity is reduced by crosslinking to limit SF conformational movement. Finally, the eukaryotic K + channel TREK2 SF exhibits essentially identical smFRET-reported ion-dependent conformations as in prokaryotic K + channels. Our results establish the generality of K + -induced SF conformational stability across the K + channel superfamily, and introduce an approach to study manipulation of channel selectivity." @default.
- W4313625062 created "2023-01-07" @default.
- W4313625062 creator A5009542131 @default.
- W4313625062 creator A5013928213 @default.
- W4313625062 creator A5036578369 @default.
- W4313625062 creator A5042664319 @default.
- W4313625062 creator A5088002796 @default.
- W4313625062 date "2023-01-06" @default.
- W4313625062 modified "2023-10-13" @default.
- W4313625062 title "Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters" @default.
- W4313625062 cites W1518056784 @default.
- W4313625062 cites W1572086689 @default.
- W4313625062 cites W1967240503 @default.
- W4313625062 cites W1967930702 @default.
- W4313625062 cites W1969129798 @default.
- W4313625062 cites W1973472018 @default.
- W4313625062 cites W1980779109 @default.
- W4313625062 cites W1985650731 @default.
- W4313625062 cites W1990897746 @default.
- W4313625062 cites W2008442844 @default.
- W4313625062 cites W2016555254 @default.
- W4313625062 cites W2023031952 @default.
- W4313625062 cites W2024012749 @default.
- W4313625062 cites W2032734987 @default.
- W4313625062 cites W2038827150 @default.
- W4313625062 cites W2041345208 @default.
- W4313625062 cites W2059625243 @default.
- W4313625062 cites W2059807927 @default.
- W4313625062 cites W2062665005 @default.
- W4313625062 cites W2066521844 @default.
- W4313625062 cites W2074610317 @default.
- W4313625062 cites W2075452701 @default.
- W4313625062 cites W2076413516 @default.
- W4313625062 cites W2079991169 @default.
- W4313625062 cites W2081989845 @default.
- W4313625062 cites W2082708471 @default.
- W4313625062 cites W2088337203 @default.
- W4313625062 cites W2101204196 @default.
- W4313625062 cites W2116778122 @default.
- W4313625062 cites W2124653618 @default.
- W4313625062 cites W2128133772 @default.
- W4313625062 cites W2132316084 @default.
- W4313625062 cites W2135379354 @default.
- W4313625062 cites W2142844453 @default.
- W4313625062 cites W2143070723 @default.
- W4313625062 cites W2164829352 @default.
- W4313625062 cites W2165394975 @default.
- W4313625062 cites W2258447134 @default.
- W4313625062 cites W2275043758 @default.
- W4313625062 cites W2337364339 @default.
- W4313625062 cites W2414191144 @default.
- W4313625062 cites W2576780822 @default.
- W4313625062 cites W2593710600 @default.
- W4313625062 cites W2607224862 @default.
- W4313625062 cites W2609394075 @default.
- W4313625062 cites W2613159616 @default.
- W4313625062 cites W2724716579 @default.
- W4313625062 cites W2766507797 @default.
- W4313625062 cites W2776223742 @default.
- W4313625062 cites W2792458540 @default.
- W4313625062 cites W2799396763 @default.
- W4313625062 cites W2883678461 @default.
- W4313625062 cites W2884177311 @default.
- W4313625062 cites W2890035512 @default.
- W4313625062 cites W2919602178 @default.
- W4313625062 cites W2980975640 @default.
- W4313625062 cites W3013007514 @default.
- W4313625062 cites W3045631794 @default.
- W4313625062 cites W3090039612 @default.
- W4313625062 cites W3095128957 @default.
- W4313625062 cites W3108987851 @default.
- W4313625062 cites W3134601812 @default.
- W4313625062 cites W3142445656 @default.
- W4313625062 cites W3152378859 @default.
- W4313625062 cites W3164055486 @default.
- W4313625062 cites W3169979759 @default.
- W4313625062 cites W3209098591 @default.
- W4313625062 cites W4220824989 @default.
- W4313625062 cites W4283798439 @default.
- W4313625062 doi "https://doi.org/10.1038/s41467-022-35756-7" @default.
- W4313625062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36609575" @default.
- W4313625062 hasPublicationYear "2023" @default.
- W4313625062 type Work @default.
- W4313625062 citedByCount "0" @default.
- W4313625062 crossrefType "journal-article" @default.
- W4313625062 hasAuthorship W4313625062A5009542131 @default.
- W4313625062 hasAuthorship W4313625062A5013928213 @default.
- W4313625062 hasAuthorship W4313625062A5036578369 @default.
- W4313625062 hasAuthorship W4313625062A5042664319 @default.
- W4313625062 hasAuthorship W4313625062A5088002796 @default.
- W4313625062 hasBestOaLocation W43136250621 @default.
- W4313625062 hasConcept C118792377 @default.
- W4313625062 hasConcept C121332964 @default.
- W4313625062 hasConcept C12554922 @default.
- W4313625062 hasConcept C161790260 @default.
- W4313625062 hasConcept C170493617 @default.
- W4313625062 hasConcept C178790620 @default.
- W4313625062 hasConcept C185592680 @default.
- W4313625062 hasConcept C50254741 @default.
- W4313625062 hasConcept C50952357 @default.