Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313626677> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4313626677 endingPage "S10" @default.
- W4313626677 startingPage "S10" @default.
- W4313626677 abstract "Maternal endothelium dysfunction is a central component to preeclampsia pathogenesis. Studies to dissect the maternal endothelial dysfunction, particularly on a patient-specific basis, is hampered by access to systemic primary arterial endothelial cells (ECs). The aim of this study was to establish a replenishable patient-specific in vitro maternal ECs model to allow robust mechanistic studies to dissect preeclampsia endothelial dysfunction. Induced pluripotent stem cells (iPSCs) derived from three women were differentiated into ECs using our recently developed protocol. The differentiated ECs were exposed to pooled banked sera from normotensive pregnancies (n=24), preeclamptic pregnancies (n=26), normotensive postpartum for non-pregnant comparison (n=22) and controls [basal media and fetal bovine serum (FBS) as a positive control]. ECs functional analyses (nitric oxide release, migration, and tube formation) were evaluated in triplicate/quadruplicate for all 3 lines. Multiple group comparisons were performed by one-way ANOVAs followed by Tukey tests. Significance was considered as P < 0.05. Patient-specific iPSCs were established and subsequently differentiated into ECs (Fig. 1). Levels of nitric oxide release were significantly lower after incubation with preeclamptic sera compared to the FBS control, and normotensive and non-pregnant (postpartum) sera treatments were also lower than FBS but higher than PE treatments (Fig. 2A). Tube formation and migration also demonstrated significantly impaired functions by preeclamptic sera compared to FBS controls (Fig. 2B and 2C). Cell viabilities were not affected by sera treatment (Fig. 2D). Similar results were obtained for all three patient-specific lines. Establishment of patient-derived iPSCs-ECs treated with pregnancy sera serves as a novel model to study maternal endothelial dysfunction. This system makes it possible to define the interplay between individual maternal endothelial health and the circulating factors that lead to preeclampsia endothelial dysfunction.View Large Image Figure ViewerDownload Hi-res image Download (PPT)" @default.
- W4313626677 created "2023-01-07" @default.
- W4313626677 creator A5004329809 @default.
- W4313626677 creator A5013796724 @default.
- W4313626677 creator A5031964598 @default.
- W4313626677 creator A5047305050 @default.
- W4313626677 creator A5047561254 @default.
- W4313626677 creator A5054450817 @default.
- W4313626677 creator A5055852366 @default.
- W4313626677 creator A5059569437 @default.
- W4313626677 date "2023-01-01" @default.
- W4313626677 modified "2023-09-26" @default.
- W4313626677 title "A novel in vitro stem cell model to study maternal endothelial function in preeclampsia" @default.
- W4313626677 doi "https://doi.org/10.1016/j.ajog.2022.11.015" @default.
- W4313626677 hasPublicationYear "2023" @default.
- W4313626677 type Work @default.
- W4313626677 citedByCount "0" @default.
- W4313626677 crossrefType "journal-article" @default.
- W4313626677 hasAuthorship W4313626677A5004329809 @default.
- W4313626677 hasAuthorship W4313626677A5013796724 @default.
- W4313626677 hasAuthorship W4313626677A5031964598 @default.
- W4313626677 hasAuthorship W4313626677A5047305050 @default.
- W4313626677 hasAuthorship W4313626677A5047561254 @default.
- W4313626677 hasAuthorship W4313626677A5054450817 @default.
- W4313626677 hasAuthorship W4313626677A5055852366 @default.
- W4313626677 hasAuthorship W4313626677A5059569437 @default.
- W4313626677 hasConcept C104317684 @default.
- W4313626677 hasConcept C107459253 @default.
- W4313626677 hasConcept C123012128 @default.
- W4313626677 hasConcept C126322002 @default.
- W4313626677 hasConcept C134018914 @default.
- W4313626677 hasConcept C145103041 @default.
- W4313626677 hasConcept C16685009 @default.
- W4313626677 hasConcept C202751555 @default.
- W4313626677 hasConcept C203014093 @default.
- W4313626677 hasConcept C2776992346 @default.
- W4313626677 hasConcept C2777218350 @default.
- W4313626677 hasConcept C2778024521 @default.
- W4313626677 hasConcept C2779234561 @default.
- W4313626677 hasConcept C2780942790 @default.
- W4313626677 hasConcept C2780972559 @default.
- W4313626677 hasConcept C519581460 @default.
- W4313626677 hasConcept C54355233 @default.
- W4313626677 hasConcept C55493867 @default.
- W4313626677 hasConcept C555293320 @default.
- W4313626677 hasConcept C71924100 @default.
- W4313626677 hasConcept C86803240 @default.
- W4313626677 hasConceptScore W4313626677C104317684 @default.
- W4313626677 hasConceptScore W4313626677C107459253 @default.
- W4313626677 hasConceptScore W4313626677C123012128 @default.
- W4313626677 hasConceptScore W4313626677C126322002 @default.
- W4313626677 hasConceptScore W4313626677C134018914 @default.
- W4313626677 hasConceptScore W4313626677C145103041 @default.
- W4313626677 hasConceptScore W4313626677C16685009 @default.
- W4313626677 hasConceptScore W4313626677C202751555 @default.
- W4313626677 hasConceptScore W4313626677C203014093 @default.
- W4313626677 hasConceptScore W4313626677C2776992346 @default.
- W4313626677 hasConceptScore W4313626677C2777218350 @default.
- W4313626677 hasConceptScore W4313626677C2778024521 @default.
- W4313626677 hasConceptScore W4313626677C2779234561 @default.
- W4313626677 hasConceptScore W4313626677C2780942790 @default.
- W4313626677 hasConceptScore W4313626677C2780972559 @default.
- W4313626677 hasConceptScore W4313626677C519581460 @default.
- W4313626677 hasConceptScore W4313626677C54355233 @default.
- W4313626677 hasConceptScore W4313626677C55493867 @default.
- W4313626677 hasConceptScore W4313626677C555293320 @default.
- W4313626677 hasConceptScore W4313626677C71924100 @default.
- W4313626677 hasConceptScore W4313626677C86803240 @default.
- W4313626677 hasIssue "1" @default.
- W4313626677 hasLocation W43136266771 @default.
- W4313626677 hasOpenAccess W4313626677 @default.
- W4313626677 hasPrimaryLocation W43136266771 @default.
- W4313626677 hasRelatedWork W1969550954 @default.
- W4313626677 hasRelatedWork W2009854143 @default.
- W4313626677 hasRelatedWork W2016328345 @default.
- W4313626677 hasRelatedWork W2072392368 @default.
- W4313626677 hasRelatedWork W2077325982 @default.
- W4313626677 hasRelatedWork W2135024115 @default.
- W4313626677 hasRelatedWork W2150096959 @default.
- W4313626677 hasRelatedWork W2162653391 @default.
- W4313626677 hasRelatedWork W2411332413 @default.
- W4313626677 hasRelatedWork W2915913567 @default.
- W4313626677 hasVolume "228" @default.
- W4313626677 isParatext "false" @default.
- W4313626677 isRetracted "false" @default.
- W4313626677 workType "article" @default.