FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313646872> ?p ?o ?g. }

• W4313646872 abstract "Abstract Background Corynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although CRISPR-based genome editing technologies have undergone immense developments in recent years, the suicide-plasmid-based approaches are still predominant for C. glutamicum genome manipulation. It is crucial to develop a simple and efficient CRISPR genome editing method for C. glutamicum . Results In this study, we developed a RecombinAtion Prior to Induced Double-strand-break (RAPID) genome editing technology for C. glutamicum , as Cpf1 cleavage was found to disrupt RecET-mediated homologous recombination (HR) of the donor template into the genome. The RAPID toolbox enabled highly efficient gene deletion and insertion, and notably, a linear DNA template was sufficient for gene deletion. Due to the simplified procedure and iterative operation ability, this methodology could be widely applied in C. glutamicum genetic manipulations. As a proof of concept, a high-yield D-pantothenic acid (vitamin B5)-producing strain was constructed, which, to the best of our knowledge, achieved the highest reported titer of 18.62 g/L from glucose only. Conclusions We developed a RecET-assisted CRISPR–Cpf1 genome editing technology for C. glutamicum that harnessed CRISPR-induced DSBs as a counterselection. This method is of great importance to C. glutamicum genome editing in terms of its practical applications, which also guides the development of CRISPR genome editing tools for other microorganisms." @default.
• W4313646872 created "2023-01-07" @default.
• W4313646872 creator A5019641117 @default.
• W4313646872 creator A5031750008 @default.
• W4313646872 creator A5032278821 @default.
• W4313646872 creator A5039933653 @default.
• W4313646872 creator A5047508906 @default.
• W4313646872 creator A5067560165 @default.
• W4313646872 creator A5068080767 @default.
• W4313646872 creator A5068916001 @default.
• W4313646872 creator A5073399950 @default.
• W4313646872 creator A5080298585 @default.
• W4313646872 date "2023-01-06" @default.
• W4313646872 modified "2023-10-18" @default.
• W4313646872 title "Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method" @default.
• W4313646872 cites W1586536230 @default.
• W4313646872 cites W1977884520 @default.
• W4313646872 cites W1994872369 @default.
• W4313646872 cites W2000046950 @default.
• W4313646872 cites W2025605247 @default.
• W4313646872 cites W2045435533 @default.
• W4313646872 cites W2048729067 @default.
• W4313646872 cites W2064815984 @default.
• W4313646872 cites W2072278470 @default.
• W4313646872 cites W2094268715 @default.
• W4313646872 cites W2113858410 @default.
• W4313646872 cites W2116137883 @default.
• W4313646872 cites W2153076508 @default.
• W4313646872 cites W2155430609 @default.
• W4313646872 cites W2165869860 @default.
• W4313646872 cites W2187650526 @default.
• W4313646872 cites W2282184745 @default.
• W4313646872 cites W2411717244 @default.
• W4313646872 cites W2498972343 @default.
• W4313646872 cites W2549989325 @default.
• W4313646872 cites W2558343263 @default.
• W4313646872 cites W2610391087 @default.
• W4313646872 cites W2693098264 @default.
• W4313646872 cites W2713682953 @default.
• W4313646872 cites W2742377280 @default.
• W4313646872 cites W2769756845 @default.
• W4313646872 cites W2769919647 @default.
• W4313646872 cites W2791153989 @default.
• W4313646872 cites W2801576592 @default.
• W4313646872 cites W2802669714 @default.
• W4313646872 cites W2884168097 @default.
• W4313646872 cites W2890736863 @default.
• W4313646872 cites W2901028097 @default.
• W4313646872 cites W2927318903 @default.
• W4313646872 cites W2943414619 @default.
• W4313646872 cites W2943959000 @default.
• W4313646872 cites W2944023328 @default.
• W4313646872 cites W2949403150 @default.
• W4313646872 cites W2955737238 @default.
• W4313646872 cites W2990758636 @default.
• W4313646872 cites W3039715109 @default.
• W4313646872 cites W3041176764 @default.
• W4313646872 cites W3082559105 @default.
• W4313646872 cites W3139134463 @default.
• W4313646872 cites W3160119089 @default.
• W4313646872 cites W3160818827 @default.
• W4313646872 cites W3188790065 @default.
• W4313646872 cites W826124742 @default.
• W4313646872 doi "https://doi.org/10.1186/s12934-023-02017-1" @default.
• W4313646872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36609377" @default.
• W4313646872 hasPublicationYear "2023" @default.
• W4313646872 type Work @default.
• W4313646872 citedByCount "3" @default.
• W4313646872 countsByYear W43136468722023 @default.
• W4313646872 crossrefType "journal-article" @default.
• W4313646872 hasAuthorship W4313646872A5019641117 @default.
• W4313646872 hasAuthorship W4313646872A5031750008 @default.
• W4313646872 hasAuthorship W4313646872A5032278821 @default.
• W4313646872 hasAuthorship W4313646872A5039933653 @default.
• W4313646872 hasAuthorship W4313646872A5047508906 @default.
• W4313646872 hasAuthorship W4313646872A5067560165 @default.
• W4313646872 hasAuthorship W4313646872A5068080767 @default.
• W4313646872 hasAuthorship W4313646872A5068916001 @default.
• W4313646872 hasAuthorship W4313646872A5073399950 @default.
• W4313646872 hasAuthorship W4313646872A5080298585 @default.
• W4313646872 hasBestOaLocation W43136468721 @default.
• W4313646872 hasConcept C102744134 @default.
• W4313646872 hasConcept C104317684 @default.
• W4313646872 hasConcept C132455925 @default.
• W4313646872 hasConcept C141231307 @default.
• W4313646872 hasConcept C144501496 @default.
• W4313646872 hasConcept C145290725 @default.
• W4313646872 hasConcept C22744801 @default.
• W4313646872 hasConcept C2776260651 @default.
• W4313646872 hasConcept C54355233 @default.
• W4313646872 hasConcept C70721500 @default.
• W4313646872 hasConcept C86803240 @default.
• W4313646872 hasConcept C98108389 @default.
• W4313646872 hasConceptScore W4313646872C102744134 @default.
• W4313646872 hasConceptScore W4313646872C104317684 @default.
• W4313646872 hasConceptScore W4313646872C132455925 @default.
• W4313646872 hasConceptScore W4313646872C141231307 @default.
• W4313646872 hasConceptScore W4313646872C144501496 @default.
• W4313646872 hasConceptScore W4313646872C145290725 @default.
• W4313646872 hasConceptScore W4313646872C22744801 @default.

• next