Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313650160> ?p ?o ?g. }
- W4313650160 endingPage "116917" @default.
- W4313650160 startingPage "116917" @default.
- W4313650160 abstract "Digital CRISPR (dCRISPR) assays are an emerging platform of molecular diagnostics. Digital platforms introduce absolute quantification and increased sensitivity to bulk CRISPR assays. With ultra-specific targeting, isothermal operation, and rapid detection, dCRISPR systems are well-prepared to lead the field of molecular diagnostics. Here we summarized the common Cas proteins used in CRISPR detection assays. The methods of digital detection and critical performance factors are examined. We formed three strategies to frame the landscape of dCRISPR systems: (1) amplification free, (2) in-partition amplification, and (3) two-stage amplification. We also compared the performance of all systems through the limit of detection (LOD), testing time, and figure of merit (FOM). This work summarizes the details of digital CRISPR platforms to guide future development. We envision that improvements to LOD and dynamic range will position dCRISPR as the leading platform for the next generation of molecular biosensing." @default.
- W4313650160 created "2023-01-07" @default.
- W4313650160 creator A5027276191 @default.
- W4313650160 creator A5055361395 @default.
- W4313650160 creator A5084755494 @default.
- W4313650160 date "2023-02-01" @default.
- W4313650160 modified "2023-10-18" @default.
- W4313650160 title "Digital CRISPR systems for the next generation of nucleic acid quantification" @default.
- W4313650160 cites W2113559596 @default.
- W4313650160 cites W2124019798 @default.
- W4313650160 cites W2143297413 @default.
- W4313650160 cites W2145303234 @default.
- W4313650160 cites W2150204651 @default.
- W4313650160 cites W2405036119 @default.
- W4313650160 cites W2409671300 @default.
- W4313650160 cites W2511569157 @default.
- W4313650160 cites W2548144690 @default.
- W4313650160 cites W2606915822 @default.
- W4313650160 cites W2613867010 @default.
- W4313650160 cites W2617378503 @default.
- W4313650160 cites W2622101209 @default.
- W4313650160 cites W2763042654 @default.
- W4313650160 cites W2777223713 @default.
- W4313650160 cites W2785904295 @default.
- W4313650160 cites W2787024379 @default.
- W4313650160 cites W2790313560 @default.
- W4313650160 cites W2800905921 @default.
- W4313650160 cites W2801566467 @default.
- W4313650160 cites W2801783434 @default.
- W4313650160 cites W2887676715 @default.
- W4313650160 cites W2888908334 @default.
- W4313650160 cites W2894605860 @default.
- W4313650160 cites W2897700601 @default.
- W4313650160 cites W2907297974 @default.
- W4313650160 cites W2908282672 @default.
- W4313650160 cites W2910181162 @default.
- W4313650160 cites W2921272926 @default.
- W4313650160 cites W2944699693 @default.
- W4313650160 cites W2951960076 @default.
- W4313650160 cites W2964838896 @default.
- W4313650160 cites W2973445868 @default.
- W4313650160 cites W2995484383 @default.
- W4313650160 cites W3007031304 @default.
- W4313650160 cites W3017334101 @default.
- W4313650160 cites W3017669300 @default.
- W4313650160 cites W3020077371 @default.
- W4313650160 cites W3060075221 @default.
- W4313650160 cites W3104540940 @default.
- W4313650160 cites W3111292947 @default.
- W4313650160 cites W3152649722 @default.
- W4313650160 cites W3155521739 @default.
- W4313650160 cites W3157156415 @default.
- W4313650160 cites W3157572888 @default.
- W4313650160 cites W3162126409 @default.
- W4313650160 cites W3162164881 @default.
- W4313650160 cites W3165397540 @default.
- W4313650160 cites W3165793603 @default.
- W4313650160 cites W3178418858 @default.
- W4313650160 cites W3179198623 @default.
- W4313650160 cites W3181270618 @default.
- W4313650160 cites W3189188210 @default.
- W4313650160 cites W3197347308 @default.
- W4313650160 cites W3215296137 @default.
- W4313650160 cites W3215993816 @default.
- W4313650160 cites W4206796073 @default.
- W4313650160 cites W4206933393 @default.
- W4313650160 cites W4210694073 @default.
- W4313650160 cites W4210960748 @default.
- W4313650160 cites W4213207818 @default.
- W4313650160 cites W4220956378 @default.
- W4313650160 cites W4224299753 @default.
- W4313650160 cites W4249742884 @default.
- W4313650160 cites W4280565528 @default.
- W4313650160 cites W4281551921 @default.
- W4313650160 cites W4282922213 @default.
- W4313650160 cites W4283586979 @default.
- W4313650160 cites W4285412195 @default.
- W4313650160 cites W4285793354 @default.
- W4313650160 doi "https://doi.org/10.1016/j.trac.2023.116917" @default.
- W4313650160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36744100" @default.
- W4313650160 hasPublicationYear "2023" @default.
- W4313650160 type Work @default.
- W4313650160 citedByCount "4" @default.
- W4313650160 countsByYear W43136501602023 @default.
- W4313650160 crossrefType "journal-article" @default.
- W4313650160 hasAuthorship W4313650160A5027276191 @default.
- W4313650160 hasAuthorship W4313650160A5055361395 @default.
- W4313650160 hasAuthorship W4313650160A5084755494 @default.
- W4313650160 hasConcept C104317684 @default.
- W4313650160 hasConcept C171250308 @default.
- W4313650160 hasConcept C192562407 @default.
- W4313650160 hasConcept C24107716 @default.
- W4313650160 hasConcept C2910367586 @default.
- W4313650160 hasConcept C41008148 @default.
- W4313650160 hasConcept C48126324 @default.
- W4313650160 hasConcept C49105822 @default.
- W4313650160 hasConcept C54355233 @default.
- W4313650160 hasConcept C552990157 @default.