FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313650223> ?p ?o ?g. }

• W4313650223 endingPage "260" @default.
• W4313650223 startingPage "260" @default.
• W4313650223 abstract "<ns3:p><ns3:bold>Background: </ns3:bold>Cross-linking mass spectrometry (XL-MS) is a powerful technology capable of yielding structural insights across the complex cellular protein interaction network. However, up to date most of the studies utilising XL-MS to characterise individual protein complexes’ topology have been carried out on over-expressed or recombinant proteins, which might not accurately represent native cellular conditions.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> We performed XL-MS using MS-cleavable crosslinker disuccinimidyl sulfoxide (DSSO) after immunoprecipitation of endogenous BRG/Brahma-associated factors (BAF) complex and co-purifying proteins. Data are available via ProteomeXchange with identifier PXD027611.</ns3:p><ns3:p> <ns3:bold>Results:</ns3:bold> Although we did not detect the expected enrichment of crosslinks within the BAF complex, we identified numerous crosslinks between three co-purifying proteins, namely Thrap3, Bclaf1 and Erh. Thrap3 and Bclaf1 are mostly disordered proteins for which no 3D structure is available. The XL data allowed us to map interaction surfaces on these proteins, which overlap with the non-disordered portions of both proteins. The identified XLs are in agreement with homology-modelled structures suggesting that the interaction surfaces are globular.</ns3:p><ns3:p> <ns3:bold>Conclusions:</ns3:bold> Our data shows that MS-cleavable crosslinker DSSO can be used to characterise in detail the topology and interaction surfaces of endogenous protein complexes without the need for overexpression. We demonstrate that Bclaf1, Erh and Thrap3 interact closely with each other, suggesting they might form a novel complex, hereby referred to as TEB complex. This data can be exploited for modelling protein-protein docking to characterise the three-dimensional structure of the complex. Endogenous XL-MS might be challenging due to crosslinker accessibility, protein complex abundance or isolation efficiency, and require further optimisation for some complexes like the BAF complex to detect a substantial number of crosslinks.</ns3:p>" @default.
• W4313650223 created "2023-01-07" @default.
• W4313650223 creator A5002940418 @default.
• W4313650223 creator A5046346909 @default.
• W4313650223 creator A5077494260 @default.
• W4313650223 creator A5087349291 @default.
• W4313650223 date "2023-01-06" @default.
• W4313650223 modified "2023-10-14" @default.
• W4313650223 title "Identifying and characterising Thrap3, Bclaf1 and Erh interactions using cross-linking mass spectrometry" @default.
• W4313650223 cites W1963679398 @default.
• W4313650223 cites W1975057169 @default.
• W4313650223 cites W1979681327 @default.
• W4313650223 cites W1979880724 @default.
• W4313650223 cites W1980721507 @default.
• W4313650223 cites W1987134040 @default.
• W4313650223 cites W1996420077 @default.
• W4313650223 cites W1998163656 @default.
• W4313650223 cites W2022861419 @default.
• W4313650223 cites W2023115652 @default.
• W4313650223 cites W2027409966 @default.
• W4313650223 cites W2037312364 @default.
• W4313650223 cites W2043274355 @default.
• W4313650223 cites W2061519290 @default.
• W4313650223 cites W2063563807 @default.
• W4313650223 cites W2071714380 @default.
• W4313650223 cites W2077917986 @default.
• W4313650223 cites W2080752012 @default.
• W4313650223 cites W2086542910 @default.
• W4313650223 cites W2086674472 @default.
• W4313650223 cites W2087087669 @default.
• W4313650223 cites W2095036253 @default.
• W4313650223 cites W2100602526 @default.
• W4313650223 cites W2107755102 @default.
• W4313650223 cites W2117020466 @default.
• W4313650223 cites W2119123131 @default.
• W4313650223 cites W2120772351 @default.
• W4313650223 cites W2127422925 @default.
• W4313650223 cites W2128729770 @default.
• W4313650223 cites W2132629607 @default.
• W4313650223 cites W2149500199 @default.
• W4313650223 cites W2154019529 @default.
• W4313650223 cites W2155201844 @default.
• W4313650223 cites W2183276865 @default.
• W4313650223 cites W2217908275 @default.
• W4313650223 cites W2312121172 @default.
• W4313650223 cites W2605676002 @default.
• W4313650223 cites W2765606289 @default.
• W4313650223 cites W2791212680 @default.
• W4313650223 cites W2883227587 @default.
• W4313650223 cites W2884190327 @default.
• W4313650223 cites W2895850607 @default.
• W4313650223 cites W2896816499 @default.
• W4313650223 cites W2899760200 @default.
• W4313650223 cites W2901312249 @default.
• W4313650223 cites W2908681332 @default.
• W4313650223 cites W2917774877 @default.
• W4313650223 cites W2938574745 @default.
• W4313650223 cites W2952714244 @default.
• W4313650223 cites W2961560189 @default.
• W4313650223 cites W2965021553 @default.
• W4313650223 cites W2966215858 @default.
• W4313650223 cites W2972560904 @default.
• W4313650223 cites W2974834003 @default.
• W4313650223 cites W2990572752 @default.
• W4313650223 cites W2995405778 @default.
• W4313650223 cites W2996217322 @default.
• W4313650223 cites W3002979061 @default.
• W4313650223 cites W3003551870 @default.
• W4313650223 cites W3005935183 @default.
• W4313650223 cites W3010823740 @default.
• W4313650223 cites W3029333022 @default.
• W4313650223 cites W3031576754 @default.
• W4313650223 cites W3091933686 @default.
• W4313650223 cites W3095583226 @default.
• W4313650223 cites W3095969348 @default.
• W4313650223 cites W3108654077 @default.
• W4313650223 cites W3109801606 @default.
• W4313650223 cites W3164627503 @default.
• W4313650223 doi "https://doi.org/10.12688/wellcomeopenres.17160.2" @default.
• W4313650223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35865489" @default.
• W4313650223 hasPublicationYear "2023" @default.
• W4313650223 type Work @default.
• W4313650223 citedByCount "0" @default.
• W4313650223 crossrefType "journal-article" @default.
• W4313650223 hasAuthorship W4313650223A5002940418 @default.
• W4313650223 hasAuthorship W4313650223A5046346909 @default.
• W4313650223 hasAuthorship W4313650223A5077494260 @default.
• W4313650223 hasAuthorship W4313650223A5087349291 @default.
• W4313650223 hasBestOaLocation W43136502231 @default.
• W4313650223 hasConcept C114614502 @default.
• W4313650223 hasConcept C162356407 @default.
• W4313650223 hasConcept C184720557 @default.
• W4313650223 hasConcept C185592680 @default.
• W4313650223 hasConcept C33923547 @default.
• W4313650223 hasConcept C43617362 @default.
• W4313650223 hasConcept C70721500 @default.
• W4313650223 hasConcept C86803240 @default.
• W4313650223 hasConceptScore W4313650223C114614502 @default.

• next