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- W4313654564 abstract "Measuring Adenosine triphosphate (ATP) provides a proxy to check compliance with IMO's ballast water D-2 standard: <10 cells mL-1 in the 10-50 μm size class. Measured with standard boiling techniques the ATP concentration in aquatic eukaryotic microorganisms is 0.6 mol m-3. Model calculations with 10-50 μm spherical organisms show their ATP content is 0.2 to 20 pg cell-1, in line with several cell lysis data. However, at 10 cells mL-1, these ATP contents lead to a maximum of only 2 to 200 pg ATP mL-1, at least 7.5× below a D-2 test kit 1500 pg ATP mL-1 upper limit. Different cell shape and ATP extraction scenarios to reach 1500 pg ATP mL-1 are discussed but remain improbable. Because cell lysis data are inconclusive, and a novel phosphoric acid-benzalkonium chloride method indicates up to 3× higher ATP concentrations, an independent test kit validation and a comparison of all three techniques are recommended." @default.
- W4313654564 created "2023-01-07" @default.
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- W4313654564 date "2023-02-01" @default.
- W4313654564 modified "2023-09-30" @default.
- W4313654564 title "The critical adenosine triphosphate (ATP) concentration in treated ballast water" @default.
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- W4313654564 doi "https://doi.org/10.1016/j.marpolbul.2022.114506" @default.
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