Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313654859> ?p ?o ?g. }
- W4313654859 endingPage "95" @default.
- W4313654859 startingPage "80" @default.
- W4313654859 abstract "Fish parvalbumins are heat-stable calcium-binding proteins that are highly cross-reactive in causing allergy symptoms in fish-sensitized patients. The reactivities of parvalbumin-specific monoclonal or polyclonal antibodies with parvalbumins of different fish species allowed their application for development of various immunoassays for allergen identification in fish samples. In this study, monoclonal antibodies (MAbs) were generated against two parvalbumins - natural Atlantic cod parvalbumin and recombinant common carp β-parvalbumin expressed in E. coli. Large collections of recombinant parvalbumins and natural allergen extracts of different fish species and other animals were used to identify the specificities of these MAbs using ELISA, Western blot, and dot blot. MAbs demonstrated different patterns of cross-reactivities with recombinant parvalbumins. Their binding affinities were affected by the addition and removal of Ca2+ ions. Moreover, all MAbs showed a broad reactivity with the target antigens in natural fish, chicken, and pork extracts. The ability of two MAbs (clones 7B2 and 3F6) to identify and isolate native parvalbumins from allergen extracts was confirmed by Western blot. Epitope mapping using recombinant fragments of Atlantic cod parvalbumin (Gad m 1) and common carp parvalbumin (Cyp c 1) revealed that 4 out of 5 MAbs recognize parvalbumin regions that contain calcium binding sites. In conclusion, the generated broadly reactive well-characterized MAbs against fish β-parvalbumins could be applied for investigation of parvalbumins of fish and other animals and their detection in allergen extracts." @default.
- W4313654859 created "2023-01-07" @default.
- W4313654859 creator A5015952809 @default.
- W4313654859 creator A5049618538 @default.
- W4313654859 creator A5054540412 @default.
- W4313654859 creator A5066093448 @default.
- W4313654859 creator A5081794814 @default.
- W4313654859 creator A5084423862 @default.
- W4313654859 date "2023-02-01" @default.
- W4313654859 modified "2023-10-16" @default.
- W4313654859 title "Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts" @default.
- W4313654859 cites W1583687578 @default.
- W4313654859 cites W1662341216 @default.
- W4313654859 cites W1789293244 @default.
- W4313654859 cites W1817311928 @default.
- W4313654859 cites W1868596950 @default.
- W4313654859 cites W1979107617 @default.
- W4313654859 cites W1983990518 @default.
- W4313654859 cites W1988028428 @default.
- W4313654859 cites W1994265169 @default.
- W4313654859 cites W1997212370 @default.
- W4313654859 cites W2006083211 @default.
- W4313654859 cites W2033054252 @default.
- W4313654859 cites W2036098271 @default.
- W4313654859 cites W2038627827 @default.
- W4313654859 cites W2041111394 @default.
- W4313654859 cites W2053768065 @default.
- W4313654859 cites W2054364273 @default.
- W4313654859 cites W2054933288 @default.
- W4313654859 cites W2057746300 @default.
- W4313654859 cites W2064607106 @default.
- W4313654859 cites W2064726912 @default.
- W4313654859 cites W2067501846 @default.
- W4313654859 cites W2073439701 @default.
- W4313654859 cites W2075967861 @default.
- W4313654859 cites W2078705390 @default.
- W4313654859 cites W2083067690 @default.
- W4313654859 cites W2086156042 @default.
- W4313654859 cites W2088743035 @default.
- W4313654859 cites W2091012997 @default.
- W4313654859 cites W2114883043 @default.
- W4313654859 cites W2162870908 @default.
- W4313654859 cites W2194693702 @default.
- W4313654859 cites W2208289816 @default.
- W4313654859 cites W2328165382 @default.
- W4313654859 cites W2418916810 @default.
- W4313654859 cites W2465068471 @default.
- W4313654859 cites W2604724340 @default.
- W4313654859 cites W2609085357 @default.
- W4313654859 cites W2748309754 @default.
- W4313654859 cites W2900944399 @default.
- W4313654859 cites W2962967228 @default.
- W4313654859 cites W2973173659 @default.
- W4313654859 cites W2973319495 @default.
- W4313654859 cites W2979819999 @default.
- W4313654859 cites W2982385251 @default.
- W4313654859 cites W3020326321 @default.
- W4313654859 cites W3045125316 @default.
- W4313654859 cites W3082473682 @default.
- W4313654859 cites W3107152179 @default.
- W4313654859 cites W3120312906 @default.
- W4313654859 cites W3136221945 @default.
- W4313654859 cites W3200439258 @default.
- W4313654859 cites W4200587414 @default.
- W4313654859 cites W4213035605 @default.
- W4313654859 doi "https://doi.org/10.1016/j.molimm.2023.01.001" @default.
- W4313654859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36621061" @default.
- W4313654859 hasPublicationYear "2023" @default.
- W4313654859 type Work @default.
- W4313654859 citedByCount "0" @default.
- W4313654859 crossrefType "journal-article" @default.
- W4313654859 hasAuthorship W4313654859A5015952809 @default.
- W4313654859 hasAuthorship W4313654859A5049618538 @default.
- W4313654859 hasAuthorship W4313654859A5054540412 @default.
- W4313654859 hasAuthorship W4313654859A5066093448 @default.
- W4313654859 hasAuthorship W4313654859A5081794814 @default.
- W4313654859 hasAuthorship W4313654859A5084423862 @default.
- W4313654859 hasBestOaLocation W43136548591 @default.
- W4313654859 hasConcept C104317684 @default.
- W4313654859 hasConcept C141547260 @default.
- W4313654859 hasConcept C147483822 @default.
- W4313654859 hasConcept C153911025 @default.
- W4313654859 hasConcept C159654299 @default.
- W4313654859 hasConcept C185592680 @default.
- W4313654859 hasConcept C195616568 @default.
- W4313654859 hasConcept C203014093 @default.
- W4313654859 hasConcept C207480886 @default.
- W4313654859 hasConcept C2776415932 @default.
- W4313654859 hasConcept C2778891732 @default.
- W4313654859 hasConcept C2780510475 @default.
- W4313654859 hasConcept C2909208804 @default.
- W4313654859 hasConcept C40767141 @default.
- W4313654859 hasConcept C505870484 @default.
- W4313654859 hasConcept C542903549 @default.
- W4313654859 hasConcept C54355233 @default.
- W4313654859 hasConcept C55493867 @default.
- W4313654859 hasConcept C86803240 @default.
- W4313654859 hasConcept C956191 @default.