Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313679924> ?p ?o ?g. }
- W4313679924 abstract "Lysine methylation modulates the function of histone and non-histone proteins, and the enzymes that add or remove lysine methylation-lysine methyltransferases (KMTs) and lysine demethylases (KDMs), respectively-are frequently mutated and dysregulated in human diseases. Identification of lysine methylation sites proteome-wide has been a critical barrier to identifying the non-histone substrates of KMTs and KDMs and for studying functions of non-histone lysine methylation. Detection of lysine methylation by mass spectrometry (MS) typically relies on the enrichment of methylated peptides by pan-methyllysine antibodies. In this study, we use peptide microarrays to show that pan-methyllysine antibodies have sequence bias, and we evaluate how the differential selectivity of these reagents impacts the detection of methylated peptides in MS-based workflows. We discovered that most commercially available pan-Kme antibodies have an in vitro sequence bias, and multiple enrichment approaches provide the most comprehensive coverage of the lysine methylome. Overall, global lysine methylation proteomics with multiple characterized pan-methyllysine antibodies resulted in the detection of 5089 lysine methylation sites on 2751 proteins from two human cell lines, nearly doubling the number of reported lysine methylation sites in the human proteome." @default.
- W4313679924 created "2023-01-08" @default.
- W4313679924 creator A5004858655 @default.
- W4313679924 creator A5021503688 @default.
- W4313679924 creator A5023078651 @default.
- W4313679924 creator A5038761014 @default.
- W4313679924 creator A5041685838 @default.
- W4313679924 creator A5059619254 @default.
- W4313679924 creator A5069297803 @default.
- W4313679924 creator A5082180734 @default.
- W4313679924 date "2023-01-07" @default.
- W4313679924 modified "2023-10-09" @default.
- W4313679924 title "Global lysine methylome profiling using systematically characterized affinity reagents" @default.
- W4313679924 cites W1007961504 @default.
- W4313679924 cites W1561283795 @default.
- W4313679924 cites W1617099866 @default.
- W4313679924 cites W1656821151 @default.
- W4313679924 cites W1979187818 @default.
- W4313679924 cites W2024588431 @default.
- W4313679924 cites W2033581736 @default.
- W4313679924 cites W2034717128 @default.
- W4313679924 cites W2036357556 @default.
- W4313679924 cites W2043274355 @default.
- W4313679924 cites W2047552911 @default.
- W4313679924 cites W2060882402 @default.
- W4313679924 cites W2063271327 @default.
- W4313679924 cites W2089680405 @default.
- W4313679924 cites W2100148142 @default.
- W4313679924 cites W2106485251 @default.
- W4313679924 cites W2107892065 @default.
- W4313679924 cites W2115746733 @default.
- W4313679924 cites W2151026975 @default.
- W4313679924 cites W2165479029 @default.
- W4313679924 cites W2289124937 @default.
- W4313679924 cites W2309996550 @default.
- W4313679924 cites W2460918441 @default.
- W4313679924 cites W2488406175 @default.
- W4313679924 cites W2547642576 @default.
- W4313679924 cites W2726958202 @default.
- W4313679924 cites W2738750209 @default.
- W4313679924 cites W2741986140 @default.
- W4313679924 cites W2742172028 @default.
- W4313679924 cites W2773025088 @default.
- W4313679924 cites W2809303501 @default.
- W4313679924 cites W2890231545 @default.
- W4313679924 cites W2901291518 @default.
- W4313679924 cites W2902755804 @default.
- W4313679924 cites W2974503966 @default.
- W4313679924 cites W2979183639 @default.
- W4313679924 cites W3016139954 @default.
- W4313679924 cites W3042747985 @default.
- W4313679924 cites W3147552980 @default.
- W4313679924 doi "https://doi.org/10.1038/s41598-022-27175-x" @default.
- W4313679924 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36611042" @default.
- W4313679924 hasPublicationYear "2023" @default.
- W4313679924 type Work @default.
- W4313679924 citedByCount "1" @default.
- W4313679924 countsByYear W43136799242023 @default.
- W4313679924 crossrefType "journal-article" @default.
- W4313679924 hasAuthorship W4313679924A5004858655 @default.
- W4313679924 hasAuthorship W4313679924A5021503688 @default.
- W4313679924 hasAuthorship W4313679924A5023078651 @default.
- W4313679924 hasAuthorship W4313679924A5038761014 @default.
- W4313679924 hasAuthorship W4313679924A5041685838 @default.
- W4313679924 hasAuthorship W4313679924A5059619254 @default.
- W4313679924 hasAuthorship W4313679924A5069297803 @default.
- W4313679924 hasAuthorship W4313679924A5082180734 @default.
- W4313679924 hasBestOaLocation W43136799241 @default.
- W4313679924 hasConcept C104317684 @default.
- W4313679924 hasConcept C150194340 @default.
- W4313679924 hasConcept C150425827 @default.
- W4313679924 hasConcept C153911025 @default.
- W4313679924 hasConcept C190727270 @default.
- W4313679924 hasConcept C22709980 @default.
- W4313679924 hasConcept C2776016237 @default.
- W4313679924 hasConcept C2776745794 @default.
- W4313679924 hasConcept C2778178838 @default.
- W4313679924 hasConcept C33288867 @default.
- W4313679924 hasConcept C515207424 @default.
- W4313679924 hasConcept C55493867 @default.
- W4313679924 hasConcept C64927066 @default.
- W4313679924 hasConcept C86803240 @default.
- W4313679924 hasConcept C91965660 @default.
- W4313679924 hasConceptScore W4313679924C104317684 @default.
- W4313679924 hasConceptScore W4313679924C150194340 @default.
- W4313679924 hasConceptScore W4313679924C150425827 @default.
- W4313679924 hasConceptScore W4313679924C153911025 @default.
- W4313679924 hasConceptScore W4313679924C190727270 @default.
- W4313679924 hasConceptScore W4313679924C22709980 @default.
- W4313679924 hasConceptScore W4313679924C2776016237 @default.
- W4313679924 hasConceptScore W4313679924C2776745794 @default.
- W4313679924 hasConceptScore W4313679924C2778178838 @default.
- W4313679924 hasConceptScore W4313679924C33288867 @default.
- W4313679924 hasConceptScore W4313679924C515207424 @default.
- W4313679924 hasConceptScore W4313679924C55493867 @default.
- W4313679924 hasConceptScore W4313679924C64927066 @default.
- W4313679924 hasConceptScore W4313679924C86803240 @default.
- W4313679924 hasConceptScore W4313679924C91965660 @default.
- W4313679924 hasFunder F4320306095 @default.
- W4313679924 hasFunder F4320332161 @default.