Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313680736> ?p ?o ?g. }
- W4313680736 endingPage "1144" @default.
- W4313680736 startingPage "1137" @default.
- W4313680736 abstract "Pregabalin is an anti-neuropathic pain drug inhibiting the α2δ subunit of the voltage-dependent calcium channel in the spinal cord. The aim of this study is to characterize the transport mechanism of pregabalin at the blood-spinal cord barrier (BSCB) by means of in vivo experiments in rats and in vitro studies using primary-cultured rat spinal cord endothelial cells. We isolated endothelial cells by culturing rat spinal cord tissue in the presence of puromycin, and confirmed the expression of BSCB markers such as Cd31, Mdr1a, and Claudin-5. The uptake of pregabalin by primary-cultured rat spinal cord endothelial cells was sodium-independent and was significantly inhibited by L-leucine, 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid, and JPH203. These results suggest the involvement of L-type amino acid transporter (LAT) 1. LAT1 mRNA and protein was expressed in primary-cultured rat spinal cord endothelial cells, which is consistent with LAT1 expression at the BSCB. In the in vivo study, the transfer of pregabalin to rat spinal cord and brain was significantly decreased by the pre-administration of branched chain amino acids (BCAAs), which are endogenous substrates of LAT1. Our results indicate that pregabalin transport across the BSCB is mediated at least in part by LAT1 and is inhibited by plasma BCAAs." @default.
- W4313680736 created "2023-01-08" @default.
- W4313680736 creator A5001992157 @default.
- W4313680736 creator A5010368815 @default.
- W4313680736 creator A5022380895 @default.
- W4313680736 creator A5022660345 @default.
- W4313680736 creator A5056392683 @default.
- W4313680736 date "2023-04-01" @default.
- W4313680736 modified "2023-10-15" @default.
- W4313680736 title "L-type Amino Acid Transporter 1 (SLC7A5)-Mediated Transport of Pregabalin at the Rat Blood-Spinal Cord Barrier and its Sensitivity to Plasma Branched-Chain Amino Acids" @default.
- W4313680736 cites W14752674 @default.
- W4313680736 cites W1964583522 @default.
- W4313680736 cites W1965138514 @default.
- W4313680736 cites W1976490782 @default.
- W4313680736 cites W1982577527 @default.
- W4313680736 cites W1994334998 @default.
- W4313680736 cites W1995544693 @default.
- W4313680736 cites W2012015068 @default.
- W4313680736 cites W2034200280 @default.
- W4313680736 cites W2040329518 @default.
- W4313680736 cites W2050502250 @default.
- W4313680736 cites W2058106407 @default.
- W4313680736 cites W2068047216 @default.
- W4313680736 cites W2068812060 @default.
- W4313680736 cites W2093158657 @default.
- W4313680736 cites W2093500966 @default.
- W4313680736 cites W2120647108 @default.
- W4313680736 cites W2123686060 @default.
- W4313680736 cites W2134225224 @default.
- W4313680736 cites W2152525754 @default.
- W4313680736 cites W2156005483 @default.
- W4313680736 cites W2249226713 @default.
- W4313680736 cites W2525642193 @default.
- W4313680736 cites W2529944834 @default.
- W4313680736 cites W2797077945 @default.
- W4313680736 cites W2874578216 @default.
- W4313680736 cites W2898891029 @default.
- W4313680736 cites W3016329961 @default.
- W4313680736 cites W3089242297 @default.
- W4313680736 cites W3093746788 @default.
- W4313680736 cites W3130700305 @default.
- W4313680736 cites W4211119836 @default.
- W4313680736 cites W64492176 @default.
- W4313680736 doi "https://doi.org/10.1016/j.xphs.2022.12.028" @default.
- W4313680736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36627052" @default.
- W4313680736 hasPublicationYear "2023" @default.
- W4313680736 type Work @default.
- W4313680736 citedByCount "0" @default.
- W4313680736 crossrefType "journal-article" @default.
- W4313680736 hasAuthorship W4313680736A5001992157 @default.
- W4313680736 hasAuthorship W4313680736A5010368815 @default.
- W4313680736 hasAuthorship W4313680736A5022380895 @default.
- W4313680736 hasAuthorship W4313680736A5022660345 @default.
- W4313680736 hasAuthorship W4313680736A5056392683 @default.
- W4313680736 hasConcept C118552586 @default.
- W4313680736 hasConcept C134018914 @default.
- W4313680736 hasConcept C150903083 @default.
- W4313680736 hasConcept C185592680 @default.
- W4313680736 hasConcept C207001950 @default.
- W4313680736 hasConcept C2776468701 @default.
- W4313680736 hasConcept C2778402981 @default.
- W4313680736 hasConcept C2780775167 @default.
- W4313680736 hasConcept C42219234 @default.
- W4313680736 hasConcept C515207424 @default.
- W4313680736 hasConcept C529278444 @default.
- W4313680736 hasConcept C55493867 @default.
- W4313680736 hasConcept C71924100 @default.
- W4313680736 hasConcept C86803240 @default.
- W4313680736 hasConcept C98274493 @default.
- W4313680736 hasConceptScore W4313680736C118552586 @default.
- W4313680736 hasConceptScore W4313680736C134018914 @default.
- W4313680736 hasConceptScore W4313680736C150903083 @default.
- W4313680736 hasConceptScore W4313680736C185592680 @default.
- W4313680736 hasConceptScore W4313680736C207001950 @default.
- W4313680736 hasConceptScore W4313680736C2776468701 @default.
- W4313680736 hasConceptScore W4313680736C2778402981 @default.
- W4313680736 hasConceptScore W4313680736C2780775167 @default.
- W4313680736 hasConceptScore W4313680736C42219234 @default.
- W4313680736 hasConceptScore W4313680736C515207424 @default.
- W4313680736 hasConceptScore W4313680736C529278444 @default.
- W4313680736 hasConceptScore W4313680736C55493867 @default.
- W4313680736 hasConceptScore W4313680736C71924100 @default.
- W4313680736 hasConceptScore W4313680736C86803240 @default.
- W4313680736 hasConceptScore W4313680736C98274493 @default.
- W4313680736 hasFunder F4320334764 @default.
- W4313680736 hasIssue "4" @default.
- W4313680736 hasLocation W43136807361 @default.
- W4313680736 hasLocation W43136807362 @default.
- W4313680736 hasOpenAccess W4313680736 @default.
- W4313680736 hasPrimaryLocation W43136807361 @default.
- W4313680736 hasRelatedWork W2022995595 @default.
- W4313680736 hasRelatedWork W2026856921 @default.
- W4313680736 hasRelatedWork W2102234164 @default.
- W4313680736 hasRelatedWork W2319724413 @default.
- W4313680736 hasRelatedWork W2320687478 @default.
- W4313680736 hasRelatedWork W2748952813 @default.
- W4313680736 hasRelatedWork W2899084033 @default.
- W4313680736 hasRelatedWork W3087738690 @default.