Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313706508> ?p ?o ?g. }
- W4313706508 endingPage "2287" @default.
- W4313706508 startingPage "2279" @default.
- W4313706508 abstract "Bacterial leaf streak disease caused by Xanthomonas translucens pv. undulosa is an economically important disease threatening wheat and barley crops around the globe. Thus far, specific PCR-based detection and identification tests for X. translucens pathovars are not available. In this study, we used comparative genomics approach to design a pathovar-specific primer pair for detection of X. translucens pv. undulosa in naturally infected seeds and its differentiation from other pathovars of the species. For this aim, complete genome sequences of strains of different X. translucens pathovars were compared and the specific PCR primer pair XtuF/XtuR was designed. These primers were strictly specific to X. translucens pv. undulosa because the expected 229-bp DNA fragment was not amplified in the closely related pathovars or in other xanthomonads, wheat-pathogenic bacteria, and other plant-pathogenic bacteria. High sensitivity of the primer pair XtuF/XtuR allowed detection of pure DNA of the pathogen in a concentration as low as 4.5 pg/μl. The pathogen was also detected in water suspension at a concentration of 8.6 × 102 CFU/ml. The PCR test was capable of detecting the pathogen in extracts of naturally infected wheat seeds at a concentration of 3.5 × 104 CFU/g while a culture-plate method was able to detect the pathogen at a concentration of 50 × 105 CFU/g of the same seeds. The PCR test developed in this study is a step forward for precise detection and identification of X. translucens pv. undulosa to prevent outbreaks of the bacterial leaf streak disease." @default.
- W4313706508 created "2023-01-08" @default.
- W4313706508 creator A5002068943 @default.
- W4313706508 creator A5007240788 @default.
- W4313706508 creator A5014885201 @default.
- W4313706508 creator A5024764285 @default.
- W4313706508 creator A5031148000 @default.
- W4313706508 creator A5047147324 @default.
- W4313706508 creator A5064615575 @default.
- W4313706508 creator A5069548662 @default.
- W4313706508 creator A5083425367 @default.
- W4313706508 date "2023-08-01" @default.
- W4313706508 modified "2023-09-26" @default.
- W4313706508 title "Pathovar-Specific PCR Method for Detection and Identification of <i>Xanthomonas translucens</i> pv. <i>undulosa</i>" @default.
- W4313706508 cites W1987891865 @default.
- W4313706508 cites W1990966571 @default.
- W4313706508 cites W2007644043 @default.
- W4313706508 cites W2019032802 @default.
- W4313706508 cites W2020845275 @default.
- W4313706508 cites W2027893618 @default.
- W4313706508 cites W2028552856 @default.
- W4313706508 cites W2032019813 @default.
- W4313706508 cites W2040800782 @default.
- W4313706508 cites W2051346598 @default.
- W4313706508 cites W2060008144 @default.
- W4313706508 cites W2063898400 @default.
- W4313706508 cites W2069104038 @default.
- W4313706508 cites W2076026353 @default.
- W4313706508 cites W2077433399 @default.
- W4313706508 cites W2092555317 @default.
- W4313706508 cites W2096093282 @default.
- W4313706508 cites W2103740237 @default.
- W4313706508 cites W2122482690 @default.
- W4313706508 cites W2122673596 @default.
- W4313706508 cites W2195001183 @default.
- W4313706508 cites W2232068000 @default.
- W4313706508 cites W2311203695 @default.
- W4313706508 cites W2406623684 @default.
- W4313706508 cites W2533849338 @default.
- W4313706508 cites W2570405601 @default.
- W4313706508 cites W2580831643 @default.
- W4313706508 cites W2618422820 @default.
- W4313706508 cites W2743218692 @default.
- W4313706508 cites W2769455880 @default.
- W4313706508 cites W2794846844 @default.
- W4313706508 cites W2917207851 @default.
- W4313706508 cites W2967553123 @default.
- W4313706508 cites W2998084944 @default.
- W4313706508 cites W2999046440 @default.
- W4313706508 cites W3000827987 @default.
- W4313706508 cites W3167894382 @default.
- W4313706508 cites W4220925133 @default.
- W4313706508 cites W4224240036 @default.
- W4313706508 cites W4281855714 @default.
- W4313706508 cites W4383312627 @default.
- W4313706508 doi "https://doi.org/10.1094/pdis-11-22-2677-sr" @default.
- W4313706508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36611242" @default.
- W4313706508 hasPublicationYear "2023" @default.
- W4313706508 type Work @default.
- W4313706508 citedByCount "1" @default.
- W4313706508 countsByYear W43137065082023 @default.
- W4313706508 crossrefType "journal-article" @default.
- W4313706508 hasAuthorship W4313706508A5002068943 @default.
- W4313706508 hasAuthorship W4313706508A5007240788 @default.
- W4313706508 hasAuthorship W4313706508A5014885201 @default.
- W4313706508 hasAuthorship W4313706508A5024764285 @default.
- W4313706508 hasAuthorship W4313706508A5031148000 @default.
- W4313706508 hasAuthorship W4313706508A5047147324 @default.
- W4313706508 hasAuthorship W4313706508A5064615575 @default.
- W4313706508 hasAuthorship W4313706508A5069548662 @default.
- W4313706508 hasAuthorship W4313706508A5083425367 @default.
- W4313706508 hasBestOaLocation W43137065082 @default.
- W4313706508 hasConcept C104317684 @default.
- W4313706508 hasConcept C178790620 @default.
- W4313706508 hasConcept C185592680 @default.
- W4313706508 hasConcept C2776460866 @default.
- W4313706508 hasConcept C2776738503 @default.
- W4313706508 hasConcept C2777563447 @default.
- W4313706508 hasConcept C2778966571 @default.
- W4313706508 hasConcept C2780679087 @default.
- W4313706508 hasConcept C2780746887 @default.
- W4313706508 hasConcept C2781463711 @default.
- W4313706508 hasConcept C49105822 @default.
- W4313706508 hasConcept C523546767 @default.
- W4313706508 hasConcept C54355233 @default.
- W4313706508 hasConcept C86803240 @default.
- W4313706508 hasConcept C89423630 @default.
- W4313706508 hasConceptScore W4313706508C104317684 @default.
- W4313706508 hasConceptScore W4313706508C178790620 @default.
- W4313706508 hasConceptScore W4313706508C185592680 @default.
- W4313706508 hasConceptScore W4313706508C2776460866 @default.
- W4313706508 hasConceptScore W4313706508C2776738503 @default.
- W4313706508 hasConceptScore W4313706508C2777563447 @default.
- W4313706508 hasConceptScore W4313706508C2778966571 @default.
- W4313706508 hasConceptScore W4313706508C2780679087 @default.
- W4313706508 hasConceptScore W4313706508C2780746887 @default.
- W4313706508 hasConceptScore W4313706508C2781463711 @default.
- W4313706508 hasConceptScore W4313706508C49105822 @default.