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• W4313784220 abstract "Background: Recent studies have demonstrated that adipose mesenchymal stem cells (AMSCs)-derived secretome (AMSC-Se) has anticancer impacts. Objectives: This study investigated the cytotoxic impacts of AMSC-Se on a colon carcinoma cell (HT-29) line. Methods: The colon cancer cells were exposed to 50 or 100 µg/mL ASMC-Se for 24 hours. MTT test had used to examine the impacts of ASMC-Se on the survival rates of the cells. Caspase activity, mRNA, and protein expression of Bax and Bcl-2 had evaluated to determine apoptosis. Results: ASMC-Se could diminish the survival of the cells concentration-dependently. The mRNA and protein expression of Bax was concentration-dependently elevated, while Bcl-2 expression decreased in the ASMC-Se group compared to the control concentration-dependently (P < 0.05). The caspase-3 and caspase-9 activities were concentration-dependently enhanced (P < 0.05), while the caspase-8 activity did not change with the AMSC-Se. Conclusions: These findings indicate that AMSC-Se effectively prevents cell growth and induces apoptosis by stimulating the intrinsic apoptotic pathway in these cells." @default.
• W4313784220 created "2023-01-08" @default.
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• W4313784220 date "2023-01-08" @default.
• W4313784220 modified "2023-10-14" @default.
• W4313784220 title "Adipose-Derived Mesenchymal Stem Cells Secretome Induces Apoptosis in Colon Carcinoma HT-29 Cells" @default.
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• W4313784220 doi "https://doi.org/10.5812/jjcmb-133934" @default.
• W4313784220 hasPublicationYear "2023" @default.
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