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- W4313812087 abstract "Abstract Background Iris lactea var. chinensis , a perennial herbaceous species, is widely distributed and has good drought tolerance traits. However, there is little information in public databases concerning this herb, so it is difficult to understand the mechanism underlying its drought tolerance. Results In this study, we used Illumina sequencing technology to conduct an RNA sequencing (RNA-seq) analysis of I. lactea var. chinensis plants under water-stressed conditions and rehydration to explore the potential mechanisms involved in plant drought tolerance. The resulting de novo assembled transcriptome revealed 126,979 unigenes, of which 44,247 were successfully annotated. Among these, 1187 differentially expressed genes (DEGs) were identified from a comparison of the water-stressed treatment and the control (CK) treatment (T/CK); there were 481 upregulated genes and 706 downregulated genes. Additionally, 275 DEGs were identified in the comparison of the rehydration treatment and the water-stressed treatment (R/T). Based on Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) analysis, the expression levels of eight randomly selected unigenes were consistent with the transcriptomic data under water-stressed and rehydration treatment, as well as in the CK. According to Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, proline metabolism-related DEGs, including those involved in the ‘proline catabolic process’, the ‘proline metabolic process’, and ‘arginine and proline metabolism’, may play important roles in plant drought tolerance. Additionally, these DEGs encoded 43 transcription factors (TFs), 46 transporters, and 22 reactive oxygen species (ROS)-scavenging system-related proteins. Biochemical analysis and histochemical detection showed that proline and ROS were accumulated under water-stressed conditions, which is consistent with the result of the transcriptomic analysis. Conclusions In summary, our transcriptomic data revealed that the drought tolerance of I. lactea var. chinensis depends on proline metabolism, the action of TFs and transporters, and a strong ROS-scavenging system. The related genes found in this study could help us understand the mechanisms underlying the drought tolerance of I. lactea var. chinensis ." @default.
- W4313812087 created "2023-01-09" @default.
- W4313812087 creator A5003852242 @default.
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- W4313812087 date "2023-01-09" @default.
- W4313812087 modified "2023-10-14" @default.
- W4313812087 title "Iris lactea var. chinensis plant drought tolerance depends on the response of proline metabolism, transcription factors, transporters and the ROS-scavenging system" @default.
- W4313812087 cites W1559510091 @default.
- W4313812087 cites W1964923689 @default.
- W4313812087 cites W1974429249 @default.
- W4313812087 cites W1975542358 @default.
- W4313812087 cites W1978487812 @default.
- W4313812087 cites W1980111781 @default.
- W4313812087 cites W1982644949 @default.
- W4313812087 cites W1983878717 @default.
- W4313812087 cites W1985761200 @default.
- W4313812087 cites W1986921310 @default.
- W4313812087 cites W1999574084 @default.
- W4313812087 cites W2000208854 @default.
- W4313812087 cites W2008951912 @default.
- W4313812087 cites W2022341861 @default.
- W4313812087 cites W2025306751 @default.
- W4313812087 cites W2026961781 @default.
- W4313812087 cites W2027847170 @default.
- W4313812087 cites W2028240222 @default.
- W4313812087 cites W2028358339 @default.
- W4313812087 cites W2028823348 @default.
- W4313812087 cites W2041851856 @default.
- W4313812087 cites W2049421589 @default.
- W4313812087 cites W2062820196 @default.
- W4313812087 cites W2066336085 @default.
- W4313812087 cites W2067965715 @default.
- W4313812087 cites W2073700480 @default.
- W4313812087 cites W2074623777 @default.
- W4313812087 cites W2075138489 @default.
- W4313812087 cites W2075149892 @default.
- W4313812087 cites W2078704305 @default.
- W4313812087 cites W2092806862 @default.
- W4313812087 cites W2094079563 @default.
- W4313812087 cites W2095538347 @default.
- W4313812087 cites W2097961562 @default.
- W4313812087 cites W2099524720 @default.
- W4313812087 cites W2103017472 @default.
- W4313812087 cites W2103148772 @default.
- W4313812087 cites W2107277218 @default.
- W4313812087 cites W2108637588 @default.
- W4313812087 cites W2113415355 @default.
- W4313812087 cites W2114639136 @default.
- W4313812087 cites W2117608012 @default.
- W4313812087 cites W2125176583 @default.
- W4313812087 cites W2126419817 @default.
- W4313812087 cites W2129984465 @default.
- W4313812087 cites W2133790733 @default.
- W4313812087 cites W2141701933 @default.
- W4313812087 cites W2145968948 @default.
- W4313812087 cites W2151201275 @default.
- W4313812087 cites W2152789354 @default.
- W4313812087 cites W2155628349 @default.
- W4313812087 cites W2156125289 @default.
- W4313812087 cites W2161251566 @default.
- W4313812087 cites W2165032496 @default.
- W4313812087 cites W2166685186 @default.
- W4313812087 cites W2171990037 @default.
- W4313812087 cites W2179438025 @default.
- W4313812087 cites W2192080449 @default.
- W4313812087 cites W2242471476 @default.
- W4313812087 cites W2275031385 @default.
- W4313812087 cites W2302721433 @default.
- W4313812087 cites W2322971516 @default.
- W4313812087 cites W2409002067 @default.
- W4313812087 cites W2463105934 @default.
- W4313812087 cites W2474124643 @default.
- W4313812087 cites W2514554472 @default.
- W4313812087 cites W2527891094 @default.
- W4313812087 cites W2555794785 @default.
- W4313812087 cites W2594573960 @default.
- W4313812087 cites W2605983233 @default.
- W4313812087 cites W2725948580 @default.
- W4313812087 cites W2767400565 @default.
- W4313812087 cites W2790204754 @default.
- W4313812087 cites W2797762632 @default.
- W4313812087 cites W2800031930 @default.
- W4313812087 cites W2845591014 @default.
- W4313812087 cites W2903314171 @default.
- W4313812087 cites W2917300272 @default.
- W4313812087 cites W2920993999 @default.
- W4313812087 cites W2968504062 @default.
- W4313812087 cites W3096828292 @default.
- W4313812087 cites W3112376646 @default.
- W4313812087 cites W3137605880 @default.
- W4313812087 doi "https://doi.org/10.1186/s12870-022-04019-4" @default.