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- W4313856739 abstract "Two lactic bacteria were used in sequential co-cultures to demineralize (DM) and deproteinize (DP) shrimp shells (SS) to obtain chitin. During the first 24 h, Lactobacillus delbrueckii performed the DM in a minimal medium containing 100 g/L SS and 50 g/L glucose. Then, three different conditions were assayed to complete DM and perform the DP stage: 1) Bifidobacterium lactis was added with 35 g/L of glucose (Ld-G → Bl-G); 2) only B. lactis was added (Ld-G → Bl); and 3) a 35 g/L pulse of glucose was added, and at 48 h, B. lactis was inoculated (Ld-G → G → Bl). The highest DM (98.63 %) and DP (88 %) were obtained using a glucose pulse in the DM step and controlling the pH value above 6.0 in the DP step. Finally, a deacetylases cocktail produced by Aspergillus niger catalyzed the deacetylation of the resulting chitin. The chitosan samples had a deacetylation degree higher than 78 % and a solubility of 25 % in 1.0 N acetic acid. The deacetylation yield was 74 % after a mild chemical treatment, with a molecular weight of 71.31 KDa. This work reports an entirely biological process to get chitin and chitosan from SS with high yields." @default.
- W4313856739 created "2023-01-10" @default.
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- W4313856739 date "2023-03-01" @default.
- W4313856739 modified "2023-10-17" @default.
- W4313856739 title "Chitin/chitosan extraction from shrimp shell waste by a completely biotechnological process" @default.
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- W4313856739 doi "https://doi.org/10.1016/j.ijbiomac.2023.123204" @default.
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