Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313893410> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4313893410 abstract "Abstract The aim of this study was to reveal mechanisms of exosomal lncRNA GAS5 derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) on epithelial-mesenchymal transition (EMT) of human peritoneal mesothelial cells (HPMCs) under high glucose (HG) conditions. HPMCs were stimulated with 2.5% glucose. The effects on EMT of HPMCs were observed by using an hUC-MSC conditioned medium (hUC-MSC-CM) and extracted exosomes. Four groups were established: ① control group,②HG group (2.5% glucose), ③conditioned medium (CM) group (2.5% glucose and 7.5% MSC-CM), and ④ exosome group (2.5% glucose and exosomes extracted from 7.5% MSC-CM), all treated for 48 h. After hUC-MSCs were transfected with GAS5 siRNA, exosomes were extracted to act on HPMCs. Western blot assay and real-time PCR were used to detect expressions of EMT markers, PTEN and Wnt/β-catenin pathway in HPMCs. Based on the real-time PCR, the changes in levels of expression of lncRNA GAS5 and miR-21 were detected. We found that HG could induce the EMT of HPMCs. Compared with the HG group, the hUC-MSC-CM could alleviate EMT of HPMCs induced by HG through exosomes. Exosomes in the hUC-MSC-CM entered HPMCs, by transferring lncRNA GAS5 to HPMCs, which down-regulates miR-21 and up-regulates PTEN, thus finally alleviating EMT of HPMCs. Wnt/β-catenin pathway plays an essential role in alleviating EMT of HPMCs by exosomes in the hUC-MSC-CM. By transferring lncRNA GAS5 to HPMCs, exosomes derived from hUC-MSCs may competitively bind to miR-21 to regulate suppression on target PTEN genes and alleviate EMT of HPMCs through Wnt/β-catenin pathway." @default.
- W4313893410 created "2023-01-10" @default.
- W4313893410 creator A5001596967 @default.
- W4313893410 creator A5019499954 @default.
- W4313893410 creator A5023543349 @default.
- W4313893410 date "2023-01-09" @default.
- W4313893410 modified "2023-10-16" @default.
- W4313893410 title "Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA GAS5 in alleviating EMT of HPMCs via Wnt/β-catenin signaling pathway" @default.
- W4313893410 cites W1995405710 @default.
- W4313893410 cites W2006419043 @default.
- W4313893410 cites W2080442169 @default.
- W4313893410 cites W2087169627 @default.
- W4313893410 cites W2088876544 @default.
- W4313893410 cites W2099369804 @default.
- W4313893410 cites W2105726520 @default.
- W4313893410 cites W2118885337 @default.
- W4313893410 cites W2145931730 @default.
- W4313893410 cites W2564771734 @default.
- W4313893410 cites W2765357081 @default.
- W4313893410 cites W2768143645 @default.
- W4313893410 cites W2774934615 @default.
- W4313893410 cites W2776878927 @default.
- W4313893410 cites W2894598387 @default.
- W4313893410 cites W2903923120 @default.
- W4313893410 cites W2909000072 @default.
- W4313893410 cites W2919824502 @default.
- W4313893410 cites W2922102878 @default.
- W4313893410 cites W2935770531 @default.
- W4313893410 cites W2936954286 @default.
- W4313893410 cites W2943695944 @default.
- W4313893410 cites W2946745008 @default.
- W4313893410 cites W2980401773 @default.
- W4313893410 cites W2987239393 @default.
- W4313893410 cites W2991252237 @default.
- W4313893410 cites W3023784496 @default.
- W4313893410 cites W3046377059 @default.
- W4313893410 cites W3087227373 @default.
- W4313893410 cites W3107526327 @default.
- W4313893410 cites W3107721317 @default.
- W4313893410 cites W3118501133 @default.
- W4313893410 cites W3146209640 @default.
- W4313893410 cites W3197007295 @default.
- W4313893410 cites W4221019900 @default.
- W4313893410 doi "https://doi.org/10.21203/rs.3.rs-2435901/v1" @default.
- W4313893410 hasPublicationYear "2023" @default.
- W4313893410 type Work @default.
- W4313893410 citedByCount "0" @default.
- W4313893410 crossrefType "posted-content" @default.
- W4313893410 hasAuthorship W4313893410A5001596967 @default.
- W4313893410 hasAuthorship W4313893410A5019499954 @default.
- W4313893410 hasAuthorship W4313893410A5023543349 @default.
- W4313893410 hasBestOaLocation W43138934101 @default.
- W4313893410 hasConcept C104317684 @default.
- W4313893410 hasConcept C127561419 @default.
- W4313893410 hasConcept C137620995 @default.
- W4313893410 hasConcept C145059251 @default.
- W4313893410 hasConcept C185592680 @default.
- W4313893410 hasConcept C198826908 @default.
- W4313893410 hasConcept C20518536 @default.
- W4313893410 hasConcept C2781261824 @default.
- W4313893410 hasConcept C28328180 @default.
- W4313893410 hasConcept C30145163 @default.
- W4313893410 hasConcept C502942594 @default.
- W4313893410 hasConcept C55493867 @default.
- W4313893410 hasConcept C62478195 @default.
- W4313893410 hasConcept C76419328 @default.
- W4313893410 hasConcept C86803240 @default.
- W4313893410 hasConcept C95444343 @default.
- W4313893410 hasConceptScore W4313893410C104317684 @default.
- W4313893410 hasConceptScore W4313893410C127561419 @default.
- W4313893410 hasConceptScore W4313893410C137620995 @default.
- W4313893410 hasConceptScore W4313893410C145059251 @default.
- W4313893410 hasConceptScore W4313893410C185592680 @default.
- W4313893410 hasConceptScore W4313893410C198826908 @default.
- W4313893410 hasConceptScore W4313893410C20518536 @default.
- W4313893410 hasConceptScore W4313893410C2781261824 @default.
- W4313893410 hasConceptScore W4313893410C28328180 @default.
- W4313893410 hasConceptScore W4313893410C30145163 @default.
- W4313893410 hasConceptScore W4313893410C502942594 @default.
- W4313893410 hasConceptScore W4313893410C55493867 @default.
- W4313893410 hasConceptScore W4313893410C62478195 @default.
- W4313893410 hasConceptScore W4313893410C76419328 @default.
- W4313893410 hasConceptScore W4313893410C86803240 @default.
- W4313893410 hasConceptScore W4313893410C95444343 @default.
- W4313893410 hasLocation W43138934101 @default.
- W4313893410 hasOpenAccess W4313893410 @default.
- W4313893410 hasPrimaryLocation W43138934101 @default.
- W4313893410 hasRelatedWork W1999306007 @default.
- W4313893410 hasRelatedWork W2357051505 @default.
- W4313893410 hasRelatedWork W2944096190 @default.
- W4313893410 hasRelatedWork W3009964850 @default.
- W4313893410 hasRelatedWork W3086820975 @default.
- W4313893410 hasRelatedWork W3093473476 @default.
- W4313893410 hasRelatedWork W3101299451 @default.
- W4313893410 hasRelatedWork W3177925119 @default.
- W4313893410 hasRelatedWork W4220920686 @default.
- W4313893410 hasRelatedWork W4281663890 @default.
- W4313893410 isParatext "false" @default.
- W4313893410 isRetracted "false" @default.
- W4313893410 workType "article" @default.