FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313897265> ?p ?o ?g. }

• W4313897265 endingPage "115" @default.
• W4313897265 startingPage "108" @default.
• W4313897265 abstract "Small Ca2+-binding protein parvalbumin possesses two strong Ca2+/Mg2+- binding sites located within two EF-hand domains. Most parvalbumins have no tryptophan residues, while cod protein contains a single tryptophan residue, which fluorescence (spectrum maximum position and fluorescence quantum yield) is highly sensitive to the Ca2+ association/dissociation.Intrinsic protein fluorescence of cod parvalbumin can be used for elucidating the mechanism of Ca2+ binding to this protein. Fluorescence of the single tryptophan residue of cod parvalbumin has been used to monitor Ca2+-induced changes in the protein, both in steady-state and kinetic mode.Steady-state fluorescence spectra of cod parvalbumin were measured using Cary Eclipse spectrofluorimeter. Stopped-flow accessories in combination with a novel high-speed spectrofluorimeter were used for measurements of kinetics of Ca2+ dissociation from cod parvalbumin after fast mixing of Ca2+-loaded protein with a chelator of divalent metal cations ethylenediaminetetraacetic acid (EDTA).The fluorescent phase plots (fluorescence intensity at a fixed wavelength plotted against a fluorescence intensity at another fixed wavelength), constructed from steady state and kinetical data, shows a break at [Ca2+]/[parvalbumin] ratio close to 1. This means that the transition passes through an intermediate state, which is a protein with one bound calcium ion. These observations indicate that the binding of Ca2+ to cod parvalbumin is sequential.The results of the present spectral study showed that the binding of Ca2+ to cod parvalbumin is a sequential process. Calcium dissociation rate constants for the two binding sites of cod parvalbumin evaluated from the kinetic data are koff1 = 1.0 s-1 and koff2 = 1.5 s-1." @default.
• W4313897265 created "2023-01-10" @default.
• W4313897265 creator A5010177887 @default.
• W4313897265 creator A5015070856 @default.
• W4313897265 creator A5039880329 @default.
• W4313897265 creator A5044611708 @default.
• W4313897265 creator A5090044927 @default.
• W4313897265 date "2023-02-01" @default.
• W4313897265 modified "2023-09-27" @default.
• W4313897265 title "Kinetics of Ca2+ Dissociation from Cod Parvalbumin Studied by Fluorescent Stopped-flow Method" @default.
• W4313897265 cites W1571243084 @default.
• W4313897265 cites W1660937480 @default.
• W4313897265 cites W1974494605 @default.
• W4313897265 cites W1985042824 @default.
• W4313897265 cites W1988613318 @default.
• W4313897265 cites W1993061998 @default.
• W4313897265 cites W2011899756 @default.
• W4313897265 cites W2027917082 @default.
• W4313897265 cites W2029154463 @default.
• W4313897265 cites W2034943035 @default.
• W4313897265 cites W2035495689 @default.
• W4313897265 cites W2057502749 @default.
• W4313897265 cites W2062912998 @default.
• W4313897265 cites W2084797262 @default.
• W4313897265 cites W2087070363 @default.
• W4313897265 cites W2087239190 @default.
• W4313897265 cites W2091279720 @default.
• W4313897265 cites W2144096695 @default.
• W4313897265 cites W2150017038 @default.
• W4313897265 cites W2154976310 @default.
• W4313897265 cites W2561337110 @default.
• W4313897265 cites W3165811305 @default.
• W4313897265 cites W3188029502 @default.
• W4313897265 doi "https://doi.org/10.2174/0929866530666230109123224" @default.
• W4313897265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36624639" @default.
• W4313897265 hasPublicationYear "2023" @default.
• W4313897265 type Work @default.
• W4313897265 citedByCount "0" @default.
• W4313897265 crossrefType "journal-article" @default.
• W4313897265 hasAuthorship W4313897265A5010177887 @default.
• W4313897265 hasAuthorship W4313897265A5015070856 @default.
• W4313897265 hasAuthorship W4313897265A5039880329 @default.
• W4313897265 hasAuthorship W4313897265A5044611708 @default.
• W4313897265 hasAuthorship W4313897265A5090044927 @default.
• W4313897265 hasConcept C102931765 @default.
• W4313897265 hasConcept C113196181 @default.
• W4313897265 hasConcept C121332964 @default.
• W4313897265 hasConcept C12554922 @default.
• W4313897265 hasConcept C141547260 @default.
• W4313897265 hasConcept C147789679 @default.
• W4313897265 hasConcept C148898269 @default.
• W4313897265 hasConcept C169760540 @default.
• W4313897265 hasConcept C178790620 @default.
• W4313897265 hasConcept C185592680 @default.
• W4313897265 hasConcept C2776706248 @default.
• W4313897265 hasConcept C43617362 @default.
• W4313897265 hasConcept C515207424 @default.
• W4313897265 hasConcept C519063684 @default.
• W4313897265 hasConcept C55493867 @default.
• W4313897265 hasConcept C62520636 @default.
• W4313897265 hasConcept C74884574 @default.
• W4313897265 hasConcept C86803240 @default.
• W4313897265 hasConcept C91881484 @default.
• W4313897265 hasConceptScore W4313897265C102931765 @default.
• W4313897265 hasConceptScore W4313897265C113196181 @default.
• W4313897265 hasConceptScore W4313897265C121332964 @default.
• W4313897265 hasConceptScore W4313897265C12554922 @default.
• W4313897265 hasConceptScore W4313897265C141547260 @default.
• W4313897265 hasConceptScore W4313897265C147789679 @default.
• W4313897265 hasConceptScore W4313897265C148898269 @default.
• W4313897265 hasConceptScore W4313897265C169760540 @default.
• W4313897265 hasConceptScore W4313897265C178790620 @default.
• W4313897265 hasConceptScore W4313897265C185592680 @default.
• W4313897265 hasConceptScore W4313897265C2776706248 @default.
• W4313897265 hasConceptScore W4313897265C43617362 @default.
• W4313897265 hasConceptScore W4313897265C515207424 @default.
• W4313897265 hasConceptScore W4313897265C519063684 @default.
• W4313897265 hasConceptScore W4313897265C55493867 @default.
• W4313897265 hasConceptScore W4313897265C62520636 @default.
• W4313897265 hasConceptScore W4313897265C74884574 @default.
• W4313897265 hasConceptScore W4313897265C86803240 @default.
• W4313897265 hasConceptScore W4313897265C91881484 @default.
• W4313897265 hasIssue "2" @default.
• W4313897265 hasLocation W43138972651 @default.
• W4313897265 hasLocation W43138972652 @default.
• W4313897265 hasOpenAccess W4313897265 @default.
• W4313897265 hasPrimaryLocation W43138972651 @default.
• W4313897265 hasRelatedWork W1966278884 @default.
• W4313897265 hasRelatedWork W2011108298 @default.
• W4313897265 hasRelatedWork W2015013178 @default.
• W4313897265 hasRelatedWork W2022209098 @default.
• W4313897265 hasRelatedWork W2049076881 @default.
• W4313897265 hasRelatedWork W2090989414 @default.
• W4313897265 hasRelatedWork W2091165889 @default.
• W4313897265 hasRelatedWork W2361656300 @default.
• W4313897265 hasRelatedWork W2801454733 @default.
• W4313897265 hasRelatedWork W83059117 @default.
• W4313897265 hasVolume "30" @default.

• next