FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313897417> ?p ?o ?g. }

• W4313897417 endingPage "100052" @default.
• W4313897417 startingPage "100052" @default.
• W4313897417 abstract "Formalin-fixed, paraffin-embedded tissues represent a majority of all biopsy specimens commonly analyzed by histologic or immunohistochemical staining with adhesive coverslips attached. Mass spectrometry (MS) has recently been used to precisely quantify proteins in samples consisting of multiple unstained formalin-fixed, paraffin-embedded sections. Here, we report an MS method to analyze proteins from a single coverslipped 4-μm section previously stained with hematoxylin and eosin, Masson trichrome, or 3,3'-diaminobenzidine-based immunohistochemical staining. We analyzed serial unstained and stained sections from non-small cell lung cancer specimens for proteins of varying abundance (PD-L1, RB1, CD73, and HLA-DRA). Coverslips were removed by soaking in xylene, and after tryptic digestion, peptides were analyzed by targeted high-resolution liquid chromatography with tandem MS with stable isotope-labeled peptide standards. The low-abundance proteins RB1 and PD-L1 were quantified in 31 and 35 of 50 total sections analyzed, respectively, whereas higher abundance CD73 and HLA-DRA were quantified in 49 and 50 sections, respectively. The inclusion of targeted β-actin measurement enabled normalization in samples where residual stain interfered with bulk protein quantitation by colorimetric assay. Measurement coefficient of variations for 5 replicate slides (hematoxylin and eosin stained vs unstained) from each block ranged from 3% to 18% for PD-L1, from 1% to 36% for RB1, 3% to 21% for CD73, and 4% to 29% for HLA-DRA. Collectively, these results demonstrate that targeted MS protein quantification can add a valuable data layer to clinical tissue specimens after assessment for standard pathology end points." @default.
• W4313897417 created "2023-01-10" @default.
• W4313897417 creator A5001830934 @default.
• W4313897417 creator A5011916171 @default.
• W4313897417 creator A5030558866 @default.
• W4313897417 creator A5037110629 @default.
• W4313897417 creator A5041656381 @default.
• W4313897417 creator A5060974381 @default.
• W4313897417 creator A5064140137 @default.
• W4313897417 creator A5071022580 @default.
• W4313897417 creator A5081535615 @default.
• W4313897417 creator A5087444311 @default.
• W4313897417 date "2023-04-01" @default.
• W4313897417 modified "2023-09-30" @default.
• W4313897417 title "Targeted Quantitative Mass Spectrometry Analysis of Protein Biomarkers From Previously Stained Single Formalin-Fixed Paraffin-Embedded Tissue Sections" @default.
• W4313897417 cites W1995407179 @default.
• W4313897417 cites W2043951056 @default.
• W4313897417 cites W2141091417 @default.
• W4313897417 cites W2191984283 @default.
• W4313897417 cites W2214455720 @default.
• W4313897417 cites W2222860631 @default.
• W4313897417 cites W2607214718 @default.
• W4313897417 cites W2895425025 @default.
• W4313897417 cites W2963173129 @default.
• W4313897417 cites W2998135460 @default.
• W4313897417 cites W3036197269 @default.
• W4313897417 cites W3080458669 @default.
• W4313897417 cites W3086251076 @default.
• W4313897417 cites W3088602607 @default.
• W4313897417 cites W3166166848 @default.
• W4313897417 cites W3197713479 @default.
• W4313897417 cites W4280644495 @default.
• W4313897417 cites W4289711170 @default.
• W4313897417 doi "https://doi.org/10.1016/j.labinv.2022.100052" @default.
• W4313897417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36870295" @default.
• W4313897417 hasPublicationYear "2023" @default.
• W4313897417 type Work @default.
• W4313897417 citedByCount "0" @default.
• W4313897417 crossrefType "journal-article" @default.
• W4313897417 hasAuthorship W4313897417A5001830934 @default.
• W4313897417 hasAuthorship W4313897417A5011916171 @default.
• W4313897417 hasAuthorship W4313897417A5030558866 @default.
• W4313897417 hasAuthorship W4313897417A5037110629 @default.
• W4313897417 hasAuthorship W4313897417A5041656381 @default.
• W4313897417 hasAuthorship W4313897417A5060974381 @default.
• W4313897417 hasAuthorship W4313897417A5064140137 @default.
• W4313897417 hasAuthorship W4313897417A5071022580 @default.
• W4313897417 hasAuthorship W4313897417A5081535615 @default.
• W4313897417 hasAuthorship W4313897417A5087444311 @default.
• W4313897417 hasBestOaLocation W43138974171 @default.
• W4313897417 hasConcept C125473707 @default.
• W4313897417 hasConcept C142724271 @default.
• W4313897417 hasConcept C153911025 @default.
• W4313897417 hasConcept C185592680 @default.
• W4313897417 hasConcept C204232928 @default.
• W4313897417 hasConcept C2781294515 @default.
• W4313897417 hasConcept C71924100 @default.
• W4313897417 hasConcept C74864618 @default.
• W4313897417 hasConcept C86803240 @default.
• W4313897417 hasConceptScore W4313897417C125473707 @default.
• W4313897417 hasConceptScore W4313897417C142724271 @default.
• W4313897417 hasConceptScore W4313897417C153911025 @default.
• W4313897417 hasConceptScore W4313897417C185592680 @default.
• W4313897417 hasConceptScore W4313897417C204232928 @default.
• W4313897417 hasConceptScore W4313897417C2781294515 @default.
• W4313897417 hasConceptScore W4313897417C71924100 @default.
• W4313897417 hasConceptScore W4313897417C74864618 @default.
• W4313897417 hasConceptScore W4313897417C86803240 @default.
• W4313897417 hasIssue "4" @default.
• W4313897417 hasLocation W43138974171 @default.
• W4313897417 hasLocation W43138974172 @default.
• W4313897417 hasOpenAccess W4313897417 @default.
• W4313897417 hasPrimaryLocation W43138974171 @default.
• W4313897417 hasRelatedWork W1535769111 @default.
• W4313897417 hasRelatedWork W2022082721 @default.
• W4313897417 hasRelatedWork W2035464821 @default.
• W4313897417 hasRelatedWork W2070285969 @default.
• W4313897417 hasRelatedWork W2132762120 @default.
• W4313897417 hasRelatedWork W2355053214 @default.
• W4313897417 hasRelatedWork W2365674884 @default.
• W4313897417 hasRelatedWork W2375803463 @default.
• W4313897417 hasRelatedWork W2803401815 @default.
• W4313897417 hasRelatedWork W2887807965 @default.
• W4313897417 hasVolume "103" @default.
• W4313897417 isParatext "false" @default.
• W4313897417 isRetracted "false" @default.
• W4313897417 workType "article" @default.