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• W4315475122 abstract "ABSTRACT Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved ~95% theoretical yield of three C 4 commodity chemicals, n -butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points." @default.
• W4315475122 created "2023-01-11" @default.
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• W4315475122 date "2023-01-10" @default.
• W4315475122 modified "2023-10-11" @default.
• W4315475122 title "A Cellular Platform for Production of C₄Monomers" @default.
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• W4315475122 doi "https://doi.org/10.1101/2023.01.09.523327" @default.
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