Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315480972> ?p ?o ?g. }
- W4315480972 abstract "Fluxes are the ultimate phenotype of metabolism and their accurate quantification is fundamental to any understanding of metabolic networks. Steady state metabolic flux analysis has been the method of choice for quantifying fluxes in heterotrophic cells, but it is unable to measure fluxes during short-lived metabolic states, such as a transient oxidative load. Isotopically non-stationary metabolic flux analysis (INST-MFA) can be performed over shorter timescales (minutes – hours) and might overcome this limitation. INST-MFA has recently been applied to photosynthesising leaves, but agriculturally important tissues such as roots and storage organs, or plants during the night are heterotrophic. Here we outline the application of INST-MFA to heterotrophic plant cells. Using INST-MFA we were able to identify changes in the fluxes supported by phosphoenolpyruvate carboxylase and malic enzyme under oxidative load, highlighting the potential of INST-MFA to measure fluxes during short-lived metabolic states. We discuss the challenges in applying INST-MFA, and highlight further development required before it can be routinely used to quantify fluxes in heterotrophic plant cells." @default.
- W4315480972 created "2023-01-11" @default.
- W4315480972 creator A5005805995 @default.
- W4315480972 creator A5064104793 @default.
- W4315480972 creator A5070014173 @default.
- W4315480972 date "2023-01-09" @default.
- W4315480972 modified "2023-09-30" @default.
- W4315480972 title "Isotopically non-stationary metabolic flux analysis of heterotrophic Arabidopsis thaliana cell cultures" @default.
- W4315480972 cites W1559527931 @default.
- W4315480972 cites W1834965185 @default.
- W4315480972 cites W1963489914 @default.
- W4315480972 cites W1968803553 @default.
- W4315480972 cites W1983989854 @default.
- W4315480972 cites W1986765709 @default.
- W4315480972 cites W1991029581 @default.
- W4315480972 cites W1995695717 @default.
- W4315480972 cites W2001313672 @default.
- W4315480972 cites W2030933109 @default.
- W4315480972 cites W2039977436 @default.
- W4315480972 cites W2054328164 @default.
- W4315480972 cites W2063763893 @default.
- W4315480972 cites W2074892828 @default.
- W4315480972 cites W2081952527 @default.
- W4315480972 cites W2092493462 @default.
- W4315480972 cites W2095386536 @default.
- W4315480972 cites W2095733998 @default.
- W4315480972 cites W2099363748 @default.
- W4315480972 cites W2100481323 @default.
- W4315480972 cites W2103135236 @default.
- W4315480972 cites W2113377630 @default.
- W4315480972 cites W2128927770 @default.
- W4315480972 cites W2134138595 @default.
- W4315480972 cites W2140674401 @default.
- W4315480972 cites W2142111857 @default.
- W4315480972 cites W2144037437 @default.
- W4315480972 cites W2256578114 @default.
- W4315480972 cites W2463648107 @default.
- W4315480972 cites W2610435987 @default.
- W4315480972 cites W2768818971 @default.
- W4315480972 cites W2806788718 @default.
- W4315480972 cites W2900591314 @default.
- W4315480972 cites W2904458690 @default.
- W4315480972 cites W2910270451 @default.
- W4315480972 cites W2944840932 @default.
- W4315480972 cites W2963865028 @default.
- W4315480972 cites W2974361058 @default.
- W4315480972 cites W2982778037 @default.
- W4315480972 cites W3003290400 @default.
- W4315480972 cites W3026797822 @default.
- W4315480972 cites W3129607641 @default.
- W4315480972 cites W4200075658 @default.
- W4315480972 cites W4200601975 @default.
- W4315480972 cites W4210365051 @default.
- W4315480972 cites W4220767642 @default.
- W4315480972 cites W4223583835 @default.
- W4315480972 cites W4292684442 @default.
- W4315480972 doi "https://doi.org/10.3389/fpls.2022.1049559" @default.
- W4315480972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36699846" @default.
- W4315480972 hasPublicationYear "2023" @default.
- W4315480972 type Work @default.
- W4315480972 citedByCount "1" @default.
- W4315480972 countsByYear W43154809722023 @default.
- W4315480972 crossrefType "journal-article" @default.
- W4315480972 hasAuthorship W4315480972A5005805995 @default.
- W4315480972 hasAuthorship W4315480972A5064104793 @default.
- W4315480972 hasAuthorship W4315480972A5070014173 @default.
- W4315480972 hasBestOaLocation W43154809721 @default.
- W4315480972 hasConcept C104317684 @default.
- W4315480972 hasConcept C111341932 @default.
- W4315480972 hasConcept C143065580 @default.
- W4315480972 hasConcept C143937172 @default.
- W4315480972 hasConcept C160941953 @default.
- W4315480972 hasConcept C167091322 @default.
- W4315480972 hasConcept C178790620 @default.
- W4315480972 hasConcept C181199279 @default.
- W4315480972 hasConcept C183688256 @default.
- W4315480972 hasConcept C185592680 @default.
- W4315480972 hasConcept C2775988993 @default.
- W4315480972 hasConcept C35195898 @default.
- W4315480972 hasConcept C39432304 @default.
- W4315480972 hasConcept C523546767 @default.
- W4315480972 hasConcept C54355233 @default.
- W4315480972 hasConcept C55493867 @default.
- W4315480972 hasConcept C59822182 @default.
- W4315480972 hasConcept C62231903 @default.
- W4315480972 hasConcept C68709404 @default.
- W4315480972 hasConcept C86803240 @default.
- W4315480972 hasConceptScore W4315480972C104317684 @default.
- W4315480972 hasConceptScore W4315480972C111341932 @default.
- W4315480972 hasConceptScore W4315480972C143065580 @default.
- W4315480972 hasConceptScore W4315480972C143937172 @default.
- W4315480972 hasConceptScore W4315480972C160941953 @default.
- W4315480972 hasConceptScore W4315480972C167091322 @default.
- W4315480972 hasConceptScore W4315480972C178790620 @default.
- W4315480972 hasConceptScore W4315480972C181199279 @default.
- W4315480972 hasConceptScore W4315480972C183688256 @default.
- W4315480972 hasConceptScore W4315480972C185592680 @default.
- W4315480972 hasConceptScore W4315480972C2775988993 @default.
- W4315480972 hasConceptScore W4315480972C35195898 @default.
- W4315480972 hasConceptScore W4315480972C39432304 @default.