FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315488170> ?p ?o ?g. }

• W4315488170 abstract "Calcium-modulated photoactivatable ratiometric integrator (CaMPARI) is a calcium ion (Ca 2+ )- and light-dependent genetically encoded fluorescent activity integrator that can capture snapshots of neuronal activity through an irreversible process known as photoconversion. This unique property was previously used to label neurons based upon their tuning properties in order to map synaptic connectivity and to record large-scale neuronal activity in freely moving mice without attaching any mechanical device to them. The latest version of CaMPARI (CaMPARI2) was engineered to enhance the contrast generated by photoconverting the green protein to the activity-dependent red form and to reduce the Ca 2+ -independent photoconversion rate compared to the first generation of CaMPARI (CaMPARI1). However, here we show that this optimization process also resulted in reduced photoconversion efficiency of active neurons in the mouse cortex and hippocampus. Through side-by-side comparison of the two CaMPARI sensors under several experimental conditions, we show that CaMPARI1 exhibits a substantially higher red-to-green ratio in active cells than CaMPARI2. In addition, we show that CaMPARI1 also functions as a more sensitive traditional Ca 2+ sensor than CaMPARI2 by producing larger activity-driven dynamic fluorescence changes in the observed neurons. Therefore, we conclude that during the optimization process of CaMPARI2, some of the sensor’s characteristics were not predicted properly by in vitro screening assays, and therefore in vivo screening and validation steps should be included in future optimization attempts to increase the predictability of screening pipelines." @default.
• W4315488170 created "2023-01-11" @default.
• W4315488170 creator A5007941480 @default.
• W4315488170 creator A5015189414 @default.
• W4315488170 creator A5018365877 @default.
• W4315488170 creator A5045064185 @default.
• W4315488170 creator A5054437859 @default.
• W4315488170 creator A5056911132 @default.
• W4315488170 creator A5083663182 @default.
• W4315488170 date "2023-01-10" @default.
• W4315488170 modified "2023-10-14" @default.
• W4315488170 title "Enhanced detection sensitivity of neuronal activity patterns using CaMPARI1 vs. CaMPARI2" @default.
• W4315488170 cites W1571164133 @default.
• W4315488170 cites W1982918139 @default.
• W4315488170 cites W1987278178 @default.
• W4315488170 cites W2017108196 @default.
• W4315488170 cites W2025515174 @default.
• W4315488170 cites W2032554925 @default.
• W4315488170 cites W2055725584 @default.
• W4315488170 cites W2080092859 @default.
• W4315488170 cites W2109234881 @default.
• W4315488170 cites W2131938193 @default.
• W4315488170 cites W2148435032 @default.
• W4315488170 cites W2160919229 @default.
• W4315488170 cites W2171332611 @default.
• W4315488170 cites W2291957660 @default.
• W4315488170 cites W2548873975 @default.
• W4315488170 cites W2561918242 @default.
• W4315488170 cites W2896422558 @default.
• W4315488170 cites W2899201459 @default.
• W4315488170 cites W2944581648 @default.
• W4315488170 cites W2950007293 @default.
• W4315488170 cites W2950849463 @default.
• W4315488170 cites W3001871339 @default.
• W4315488170 cites W3111521801 @default.
• W4315488170 cites W3207153320 @default.
• W4315488170 cites W4213059771 @default.
• W4315488170 cites W4225489826 @default.
• W4315488170 cites W4281890878 @default.
• W4315488170 cites W4294214781 @default.
• W4315488170 cites W4294251987 @default.
• W4315488170 cites W4294898454 @default.
• W4315488170 cites W4308445192 @default.
• W4315488170 doi "https://doi.org/10.3389/fnins.2022.1055554" @default.
• W4315488170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36704000" @default.
• W4315488170 hasPublicationYear "2023" @default.
• W4315488170 type Work @default.
• W4315488170 citedByCount "0" @default.
• W4315488170 crossrefType "journal-article" @default.
• W4315488170 hasAuthorship W4315488170A5007941480 @default.
• W4315488170 hasAuthorship W4315488170A5015189414 @default.
• W4315488170 hasAuthorship W4315488170A5018365877 @default.
• W4315488170 hasAuthorship W4315488170A5045064185 @default.
• W4315488170 hasAuthorship W4315488170A5054437859 @default.
• W4315488170 hasAuthorship W4315488170A5056911132 @default.
• W4315488170 hasAuthorship W4315488170A5083663182 @default.
• W4315488170 hasBestOaLocation W43154881701 @default.
• W4315488170 hasConcept C120665830 @default.
• W4315488170 hasConcept C121332964 @default.
• W4315488170 hasConcept C12554922 @default.
• W4315488170 hasConcept C127413603 @default.
• W4315488170 hasConcept C150903083 @default.
• W4315488170 hasConcept C169760540 @default.
• W4315488170 hasConcept C178790620 @default.
• W4315488170 hasConcept C185592680 @default.
• W4315488170 hasConcept C186060115 @default.
• W4315488170 hasConcept C207001950 @default.
• W4315488170 hasConcept C21200559 @default.
• W4315488170 hasConcept C24326235 @default.
• W4315488170 hasConcept C2776032975 @default.
• W4315488170 hasConcept C2776257435 @default.
• W4315488170 hasConcept C41008148 @default.
• W4315488170 hasConcept C519063684 @default.
• W4315488170 hasConcept C74254510 @default.
• W4315488170 hasConcept C76155785 @default.
• W4315488170 hasConcept C79518650 @default.
• W4315488170 hasConcept C86803240 @default.
• W4315488170 hasConcept C91881484 @default.
• W4315488170 hasConceptScore W4315488170C120665830 @default.
• W4315488170 hasConceptScore W4315488170C121332964 @default.
• W4315488170 hasConceptScore W4315488170C12554922 @default.
• W4315488170 hasConceptScore W4315488170C127413603 @default.
• W4315488170 hasConceptScore W4315488170C150903083 @default.
• W4315488170 hasConceptScore W4315488170C169760540 @default.
• W4315488170 hasConceptScore W4315488170C178790620 @default.
• W4315488170 hasConceptScore W4315488170C185592680 @default.
• W4315488170 hasConceptScore W4315488170C186060115 @default.
• W4315488170 hasConceptScore W4315488170C207001950 @default.
• W4315488170 hasConceptScore W4315488170C21200559 @default.
• W4315488170 hasConceptScore W4315488170C24326235 @default.
• W4315488170 hasConceptScore W4315488170C2776032975 @default.
• W4315488170 hasConceptScore W4315488170C2776257435 @default.
• W4315488170 hasConceptScore W4315488170C41008148 @default.
• W4315488170 hasConceptScore W4315488170C519063684 @default.
• W4315488170 hasConceptScore W4315488170C74254510 @default.
• W4315488170 hasConceptScore W4315488170C76155785 @default.
• W4315488170 hasConceptScore W4315488170C79518650 @default.
• W4315488170 hasConceptScore W4315488170C86803240 @default.
• W4315488170 hasConceptScore W4315488170C91881484 @default.
• W4315488170 hasFunder F4320332161 @default.

• next