Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315566112> ?p ?o ?g. }
- W4315566112 endingPage "163" @default.
- W4315566112 startingPage "163" @default.
- W4315566112 abstract "Cultured primary progenitor tenocytes in lyophilized form were previously shown to possess intrinsic antioxidant properties and hyaluronan-based hydrogel viscosity-modulating effects in vitro. The aim of this study was to prepare and functionally characterize several stabilized (lyophilized) cell-free progenitor tenocyte extracts for inclusion in cytotherapy-inspired complex injectable preparations. Fractionation and sterilization methods were included in specific biotechnological manufacturing workflows of such extracts. Comparative and functional-oriented characterizations of the various extracts were performed using several orthogonal descriptive, colorimetric, rheological, mechanical, and proteomic readouts. Specifically, an optimal sugar-based (saccharose/dextran) excipient formula was retained to produce sterilizable cytotherapeutic derivatives with appropriate functions. It was shown that extracts containing soluble cell-derived fractions possessed conserved and significant antioxidant properties (TEAC) compared to the freshly harvested cellular starting materials. Progenitor tenocyte extracts submitted to sub-micron filtration (0.22 µm) and 60Co gamma irradiation terminal sterilization (5−50 kGy) were shown to retain significant antioxidant properties and hyaluronan-based hydrogel viscosity modulating effects. Hydrogel combination products displayed important efficacy-related characteristics (friction modulation, tendon bioadhesivity) with significant (p < 0.05) protective effects of the cellular extracts in oxidative environments. Overall, the present study sets forth robust control methodologies (antioxidant assays, H2O2-challenged rheological setups) for stabilized cell-free progenitor tenocyte extracts. Importantly, it was shown that highly sensitive phases of cytotherapeutic derivative manufacturing process development (purification, terminal sterilization) allowed for the conservation of critical biological extract attributes." @default.
- W4315566112 created "2023-01-11" @default.
- W4315566112 creator A5007930836 @default.
- W4315566112 creator A5009943779 @default.
- W4315566112 creator A5025087607 @default.
- W4315566112 creator A5032987278 @default.
- W4315566112 creator A5037215950 @default.
- W4315566112 creator A5040248261 @default.
- W4315566112 creator A5040290370 @default.
- W4315566112 creator A5049777995 @default.
- W4315566112 creator A5056735770 @default.
- W4315566112 creator A5070898403 @default.
- W4315566112 creator A5073925656 @default.
- W4315566112 creator A5075595632 @default.
- W4315566112 creator A5078685352 @default.
- W4315566112 creator A5079144775 @default.
- W4315566112 date "2023-01-10" @default.
- W4315566112 modified "2023-10-18" @default.
- W4315566112 title "Lyophilized Progenitor Tenocyte Extracts: Sterilizable Cytotherapeutic Derivatives with Antioxidant Properties and Hyaluronan Hydrogel Functionalization Effects" @default.
- W4315566112 cites W1582833462 @default.
- W4315566112 cites W1963566022 @default.
- W4315566112 cites W1966165596 @default.
- W4315566112 cites W1977784832 @default.
- W4315566112 cites W1989484219 @default.
- W4315566112 cites W1991683216 @default.
- W4315566112 cites W1996054586 @default.
- W4315566112 cites W2001019626 @default.
- W4315566112 cites W2011461303 @default.
- W4315566112 cites W2015617018 @default.
- W4315566112 cites W2026270542 @default.
- W4315566112 cites W2034642786 @default.
- W4315566112 cites W2041442685 @default.
- W4315566112 cites W2045653193 @default.
- W4315566112 cites W2058467705 @default.
- W4315566112 cites W2071512379 @default.
- W4315566112 cites W2091779743 @default.
- W4315566112 cites W2092420706 @default.
- W4315566112 cites W2093523807 @default.
- W4315566112 cites W2095309244 @default.
- W4315566112 cites W2120976021 @default.
- W4315566112 cites W2136530946 @default.
- W4315566112 cites W2137986174 @default.
- W4315566112 cites W2139208086 @default.
- W4315566112 cites W2142583303 @default.
- W4315566112 cites W2210874914 @default.
- W4315566112 cites W2214374991 @default.
- W4315566112 cites W2318905955 @default.
- W4315566112 cites W2339391364 @default.
- W4315566112 cites W2420074614 @default.
- W4315566112 cites W2427990163 @default.
- W4315566112 cites W2435773353 @default.
- W4315566112 cites W2436598855 @default.
- W4315566112 cites W2466651864 @default.
- W4315566112 cites W2520273052 @default.
- W4315566112 cites W2559943707 @default.
- W4315566112 cites W2581173027 @default.
- W4315566112 cites W2614812651 @default.
- W4315566112 cites W2621143848 @default.
- W4315566112 cites W2754094659 @default.
- W4315566112 cites W2791025588 @default.
- W4315566112 cites W2794995305 @default.
- W4315566112 cites W2802744107 @default.
- W4315566112 cites W2804602311 @default.
- W4315566112 cites W2805419538 @default.
- W4315566112 cites W2811215602 @default.
- W4315566112 cites W2852757835 @default.
- W4315566112 cites W2854110447 @default.
- W4315566112 cites W2888683578 @default.
- W4315566112 cites W2889861201 @default.
- W4315566112 cites W2905016320 @default.
- W4315566112 cites W2917865945 @default.
- W4315566112 cites W2920825990 @default.
- W4315566112 cites W2923763035 @default.
- W4315566112 cites W2930554983 @default.
- W4315566112 cites W2946885849 @default.
- W4315566112 cites W2966611492 @default.
- W4315566112 cites W2983375732 @default.
- W4315566112 cites W2995427659 @default.
- W4315566112 cites W2997503897 @default.
- W4315566112 cites W3014329217 @default.
- W4315566112 cites W3016097108 @default.
- W4315566112 cites W3018629020 @default.
- W4315566112 cites W3127345941 @default.
- W4315566112 cites W3135642215 @default.
- W4315566112 cites W3147935472 @default.
- W4315566112 cites W3149788554 @default.
- W4315566112 cites W3163682901 @default.
- W4315566112 cites W3169314400 @default.
- W4315566112 cites W3193284978 @default.
- W4315566112 cites W3201820402 @default.
- W4315566112 cites W3209486917 @default.
- W4315566112 cites W4200272681 @default.
- W4315566112 cites W4200526161 @default.
- W4315566112 cites W4205885712 @default.
- W4315566112 cites W4206315850 @default.
- W4315566112 cites W4230347798 @default.
- W4315566112 cites W4292528167 @default.
- W4315566112 cites W4310172098 @default.