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• W4315652065 endingPage "696" @default.
• W4315652065 startingPage "684" @default.
• W4315652065 abstract "Adherent cells use actomyosin contractility to generate mechanical force and to sense the physical properties of their environment, with dramatic consequences for migration, division, differentiation, and fate. However, the organization of the actomyosin system within cells is highly variable, with its assembly and function being controlled by small GTPases from the Rho family. To understand better how activation of these regulators translates into cell-scale force generation in the context of different physical environments, here we combine recent advances in non-neuronal optogenetics with micropatterning and traction force microscopy on soft elastic substrates. We find that, after whole-cell RhoA activation by the CRY2/CIBN optogenetic system with a short pulse of 100 ms, single cells contract on a minute timescale in proportion to their original traction force, before returning to their original tension setpoint with near perfect precision, on a longer timescale of several minutes. To decouple the biochemical and mechanical elements of this response, we introduce a mathematical model that is parametrized by fits to the dynamics of the substrate deformation energy. We find that the RhoA response builds up quickly on a timescale of 20 s, but decays slowly on a timescale of 50 s. The larger the cells and the more polarized their actin cytoskeleton, the more substrate deformation energy is generated. RhoA activation starts to saturate if optogenetic pulse length exceeds 50 ms, revealing the intrinsic limits of biochemical activation. Together our results suggest that adherent cells establish tensional homeostasis by the RhoA system, but that the setpoint and the dynamics around it are strongly determined by cell size and the architecture of the actin cytoskeleton, which both are controlled by the extracellular environment." @default.
• W4315652065 created "2023-01-12" @default.
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• W4315652065 date "2023-02-01" @default.
• W4315652065 modified "2023-10-01" @default.
• W4315652065 title "Cell size and actin architecture determine force generation in optogenetically activated cells" @default.
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• W4315652065 doi "https://doi.org/10.1016/j.bpj.2023.01.011" @default.
• W4315652065 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36635962" @default.
• W4315652065 hasPublicationYear "2023" @default.
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