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• W4315698928 abstract "first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula † by Vikas Gautam 1,*, Anandika Suryavanshi 1, Naushad Ahmad Shah 2, Kumar Gaurav Bajpai 2,*, Syed Shabihe Raza Baqri 2, Taiyab Shamshad Naqvi 2 and Anand Murari Saxena 1 1 Department of Zoology, University of Lucknow, Lucknow 226007, India 2 Department of Zoology, Shia P.G. College, Sitapur Road, Lucknow 226020, India * Authors to whom correspondence should be addressed. † Presented at the 2nd International Electronic Conference on Biomolecules: Biomacromolecules and the Modern World Challenges, 1–15 November 2022; Available online: https://iecbm2022.sciforum.net/. Biol. Life Sci. Forum 2022, 20(1), 32; https://doi.org/10.3390/IECBM2022-13694 Published: 17 November 2022 (This article belongs to the Proceedings of The 2nd International Electronic Conference on Biomolecules: Biomacromolecules and the Modern World Challenges) Download Download PDF Download PDF with Cover Download XML Download Supplementary Material Versions Notes Keywords: phytochemical; cucurbits; soxhlet method; free radical scavenging activity; antioxidants; oxidative stress Background—TC, LA and MCM plants(family: Cucurbitaceae) are widely used in traditional medicine and are important sources of vegetables in the world. Many of these are known to have important medicinal properties and have been recommended in traditional medicine for various ailments [1]. Objectives—This study aimed to evaluate the phytochemical constituents of test plants (TA, LA and MCM) of 95% ethanolic whole-plant extracts, as well as to study the antioxidant activity using DPPH assay.Materials and Methods—The plants were powdered and mechanically extracted using the soxhlet apparatus [2], which was followed by phytochemical screening of the extracts. Various classes of phytochemicals (viz., alkaloids, phenols, steroids, glycosides and saponins) were screened using standard methods [3,4,5]. The antioxidant activity was determined using DPPH assay [6]. Results—The phytochemical screening revealed the presence of glycosides in TC, saponins in MCM and LA and alkaloids in TC and MC plants. However, phenols and steroids were found in all three plant species (TC, LA and MCM). The DPPH assay to test the antioxidant activity involved the measurement of IC50 and percentage inhibition with respect to AA. Results showed that the DPPH free radicals were scavenged by all the extracts in a concentration-dependent manner. Conclusions—These dietary cucurbits showed appreciable antioxidant activity and are good sources of natural antioxidants. Future pharmaceutical uses can be deduced from these findings. Supplementary MaterialsThe presentation material of this work is available online at https://www.mdpi.com/article/10.3390/IECBM2022-13694/s1.Author ContributionsConceptualization, V.G.; methodology, V.G. and A.S.; software, V.G.; validation, V.G., A.S. and N.A.S.; formal analysis, S.S.R.B.; investigation, V.G.; resources, A.M.S.; data curation, N.A.S. and K.G.B.; writing—original draft preparation, V.G.; writing—review and editing, S.S.R.B. and T.S.N.; visualization, V.G. and A.S.; supervision, A.M.S.; project administration, V.G. and K.G.B. All authors have read and agreed to the published version of the manuscript.FundingThis research received no external funding.Institutional Review Board StatementNot applicable.Informed Consent StatementNot applicable.Data Availability StatementThe data presented in this study are available in Supplementary Material.Conflicts of InterestThe authors declare no conflict of interest.AbbreviationsTC: Trichosanthes cucumerina, LA: Luffa acutangula, MCM: Momordica charantia-muricata, DPPH: 2,2-Diphenyl-1-picrylhydrazyl, AA: Ascorbic Acid.ReferencesBusuioc, A.C.; Botezatu, A.-V.D.; Furdui, B.; Vinatoru, C.; Maggi, F.; Caprioli, G.; Dinica, R.-M. Comparative Study of the Chemical Compositions and Antioxidant Activities of Fresh Juices from Romanian Cucurbitaceae Varieties. Molecules 2020, 25, 5468. [Google Scholar] [CrossRef] [PubMed]Suryavanshi, A.; Gautam, V.; Saxena, A.M.; Panjwani, D.; Kumar, S. Evaluation of the Anti-diabetic Potential of Rumex vesicarius L. in normal and Streptozotocin induced Diabetic Rats. Res. J. Biotechnol. 2022, 17, 63–67. [Google Scholar] [CrossRef]Harborne, A.J. Phytochemical Methods a Guide to Modern Techniques of Plant Análisis; Springer: Dordrecht, The Netherlands, 1998. [Google Scholar]Harborne, J.B. Methods of plant analysis. In Phytochemical Methods; Springer: Dordrecht, The Netherlands, 1984; pp. 1–36. [Google Scholar] [CrossRef]Harborne, J.B. Phenolic compounds. In Phytochemical Methods; Springer: Dordrecht, The Netherlands, 1973; pp. 33–88. [Google Scholar]Koleva, I.I.; Van Beek, T.A.; Linssen, J.P.; Groot, A.D.; Evstatieva, L.N. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem. Anal. 2002, 13, 8–17. [Google Scholar] [CrossRef] [PubMed]Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Gautam, V.; Suryavanshi, A.; Shah, N.A.; Bajpai, K.G.; Baqri, S.S.R.; Naqvi, T.S.; Saxena, A.M. Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula. Biol. Life Sci. Forum 2022, 20, 32. https://doi.org/10.3390/IECBM2022-13694 AMA Style Gautam V, Suryavanshi A, Shah NA, Bajpai KG, Baqri SSR, Naqvi TS, Saxena AM. Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula. Biology and Life Sciences Forum. 2022; 20(1):32. https://doi.org/10.3390/IECBM2022-13694 Chicago/Turabian Style Gautam, Vikas, Anandika Suryavanshi, Naushad Ahmad Shah, Kumar Gaurav Bajpai, Syed Shabihe Raza Baqri, Taiyab Shamshad Naqvi, and Anand Murari Saxena. 2022. Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula Biology and Life Sciences Forum 20, no. 1: 32. https://doi.org/10.3390/IECBM2022-13694 Find Other Styles Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view." @default.
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