Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315702471> ?p ?o ?g. }
- W4315702471 endingPage "3803" @default.
- W4315702471 startingPage "3791" @default.
- W4315702471 abstract "Under healthy conditions, pro- and anti-phagocytic signals are balanced. Cluster of Differentiation 47 (CD47) is believed to act as an anti-phagocytic marker that is highly expressed on multiple types of human cancer cells including acute myeloid leukemia (AML) and lung and liver carcinomas, allowing them to escape phagocytosis by macrophages. Downregulating CD47 on cancer cells discloses calreticulin (CRT) to macrophages and recovers their phagocytic activity. Herein, we postulate that using a modified graphene oxide (GO) carrier to deliver small interfering RNA (siRNA) CD47 (CD47_siRNA) in AML, A549 lung, and HepG2 liver cancer cells in co-culture in vitro will silence CD47 and flag cancer cells for CRT-mediated phagocytosis. Results showed a high knockdown efficiency of CD47 and a significant increase in CRT levels simultaneously by using GO formulation as carriers in all used cancer cell lines. The presence of CRT on cancer cells was significantly higher than levels before knockdown of CD47 and was required to achieve phagocytosis in co-culture with human macrophages. Lipid nanoparticles (LNPs) and modified boron nitride nanotubes (BNPs) were used to carry CD47_siRNA, and the knockdown efficiency values of CD47 were compared in three cancer cells in co-culture, with an achieved knockdown efficiency of >95% using LNPs as carriers. Interestingly, the high efficiency of CD47 knockdown was obtained by using the LNPs and BNP carriers; however, an increase in CRT levels on cancer cells was not required for phagocytosis to happen in co-culture with human macrophages, indicating other pathways' involvement in the phagocytosis process. These findings highlight the roles of 2D (graphene oxide), 1D (boron nitride nanotube), and 0D (lipid nanoparticle) carriers for the delivery of siRNA to eliminate cancer cells in co-culture, likely through different phagocytosis pathways in multiple types of human cancer cells. Moreover, these results provide an explanation of immune therapies that target CD47 and the potential use of these carriers in screening drugs for such therapies in vitro." @default.
- W4315702471 created "2023-01-12" @default.
- W4315702471 creator A5009559290 @default.
- W4315702471 creator A5033380723 @default.
- W4315702471 creator A5054166832 @default.
- W4315702471 creator A5062256395 @default.
- W4315702471 creator A5079450252 @default.
- W4315702471 date "2023-01-12" @default.
- W4315702471 modified "2023-10-18" @default.
- W4315702471 title "Elimination of Cancer Cells in Co-Culture: Role of Different Nanocarriers in Regulation of CD47 and Calreticulin-Induced Phagocytosis" @default.
- W4315702471 cites W1966217032 @default.
- W4315702471 cites W1972740211 @default.
- W4315702471 cites W1977160822 @default.
- W4315702471 cites W1991245842 @default.
- W4315702471 cites W2010993726 @default.
- W4315702471 cites W2011076421 @default.
- W4315702471 cites W2034739637 @default.
- W4315702471 cites W2035277852 @default.
- W4315702471 cites W2040344608 @default.
- W4315702471 cites W2063721547 @default.
- W4315702471 cites W2068052762 @default.
- W4315702471 cites W2083249633 @default.
- W4315702471 cites W2086111409 @default.
- W4315702471 cites W2093705034 @default.
- W4315702471 cites W2095472825 @default.
- W4315702471 cites W2103746815 @default.
- W4315702471 cites W2120218032 @default.
- W4315702471 cites W2132752213 @default.
- W4315702471 cites W2135588100 @default.
- W4315702471 cites W2138194691 @default.
- W4315702471 cites W2142541925 @default.
- W4315702471 cites W2169550645 @default.
- W4315702471 cites W2179769347 @default.
- W4315702471 cites W2271663961 @default.
- W4315702471 cites W2296461681 @default.
- W4315702471 cites W2332132175 @default.
- W4315702471 cites W2339957651 @default.
- W4315702471 cites W2413193118 @default.
- W4315702471 cites W2420216867 @default.
- W4315702471 cites W2424135073 @default.
- W4315702471 cites W2474278012 @default.
- W4315702471 cites W2511001425 @default.
- W4315702471 cites W2586051342 @default.
- W4315702471 cites W2592634675 @default.
- W4315702471 cites W2600400434 @default.
- W4315702471 cites W2605934824 @default.
- W4315702471 cites W2618007160 @default.
- W4315702471 cites W2793563888 @default.
- W4315702471 cites W2797974163 @default.
- W4315702471 cites W2810909862 @default.
- W4315702471 cites W2811088957 @default.
- W4315702471 cites W2885802322 @default.
- W4315702471 cites W2891429615 @default.
- W4315702471 cites W2900590704 @default.
- W4315702471 cites W2923900199 @default.
- W4315702471 cites W2930725984 @default.
- W4315702471 cites W2946682034 @default.
- W4315702471 cites W2960364238 @default.
- W4315702471 cites W2963065854 @default.
- W4315702471 cites W2968060972 @default.
- W4315702471 cites W2969549827 @default.
- W4315702471 cites W2969606205 @default.
- W4315702471 cites W2985872650 @default.
- W4315702471 cites W2996345353 @default.
- W4315702471 cites W3006110498 @default.
- W4315702471 cites W3010791964 @default.
- W4315702471 cites W3016223952 @default.
- W4315702471 cites W3021257912 @default.
- W4315702471 cites W3035711571 @default.
- W4315702471 cites W3048526794 @default.
- W4315702471 cites W3090709319 @default.
- W4315702471 cites W3090984746 @default.
- W4315702471 cites W3093405310 @default.
- W4315702471 cites W3118244431 @default.
- W4315702471 cites W3118886838 @default.
- W4315702471 cites W3135732116 @default.
- W4315702471 cites W3196861882 @default.
- W4315702471 cites W3203192686 @default.
- W4315702471 cites W4200596143 @default.
- W4315702471 cites W4200599268 @default.
- W4315702471 cites W4210285619 @default.
- W4315702471 cites W4214777832 @default.
- W4315702471 cites W4281791283 @default.
- W4315702471 cites W4283071079 @default.
- W4315702471 cites W4294898322 @default.
- W4315702471 cites W47528747 @default.
- W4315702471 doi "https://doi.org/10.1021/acsami.2c19311" @default.
- W4315702471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36632842" @default.
- W4315702471 hasPublicationYear "2023" @default.
- W4315702471 type Work @default.
- W4315702471 citedByCount "1" @default.
- W4315702471 countsByYear W43157024712023 @default.
- W4315702471 crossrefType "journal-article" @default.
- W4315702471 hasAuthorship W4315702471A5009559290 @default.
- W4315702471 hasAuthorship W4315702471A5033380723 @default.
- W4315702471 hasAuthorship W4315702471A5054166832 @default.
- W4315702471 hasAuthorship W4315702471A5062256395 @default.
- W4315702471 hasAuthorship W4315702471A5079450252 @default.