FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315853533> ?p ?o ?g. }

• W4315853533 endingPage "304" @default.
• W4315853533 startingPage "304" @default.
• W4315853533 abstract "G-protein-coupled receptors (GPCRs) are the largest family of cell surface receptors. They modulate key physiological functions and are required in diverse developmental processes including embryogenesis, but their role in pluripotency maintenance and acquisition during the reprogramming towards hiPSCs draws little attention. Meanwhile, it is known that more than 106 GPCRs are overexpressed in human pluripotent stem cells (hPSCs). Previously, to identify novel effectors of reprogramming, we performed a high-throughput RNA interference (RNAi) screening assay and identified adhesion GPCR, GPR123, as a potential reprogramming effector. Its role has not been explored before. Herein, by employing GPR123 RNAi we addressed the role of GPR123 for hPSCs. The suppression of GPR123 in hPSCs leads to the loss of pluripotency and differentiation, impacted colony morphology, accumulation of cells at the G2 phase of the cell cycle, and absence of the scratch closure. Application of the GPR123 RNAi at the initiation stage of reprogramming leads to a decrease in the percentage of the “true” hiPSC colonies, a drop in E-cadherin expression, a decrease in the percentage of NANOG+ nuclei, and the absence of actin cytoskeleton remodeling. Together this leads to the absence of the alkaline-phosphatase-positive hiPSCs colonies on the 18th day of the reprogramming process. Overall, these data indicate for the first time the essential role of GPR123 in the maintenance and acquisition of pluripotency." @default.
• W4315853533 created "2023-01-13" @default.
• W4315853533 creator A5003707560 @default.
• W4315853533 creator A5010257496 @default.
• W4315853533 creator A5019795857 @default.
• W4315853533 creator A5023950095 @default.
• W4315853533 creator A5029609758 @default.
• W4315853533 creator A5039303359 @default.
• W4315853533 creator A5059821769 @default.
• W4315853533 creator A5064406709 @default.
• W4315853533 creator A5082089215 @default.
• W4315853533 date "2023-01-13" @default.
• W4315853533 modified "2023-09-24" @default.
• W4315853533 title "Essential Role of Adhesion GPCR, GPR123, for Human Pluripotent Stem Cells and Reprogramming towards Pluripotency" @default.
• W4315853533 cites W1600024584 @default.
• W4315853533 cites W1964851679 @default.
• W4315853533 cites W1966021289 @default.
• W4315853533 cites W1966354110 @default.
• W4315853533 cites W1966497932 @default.
• W4315853533 cites W1972760976 @default.
• W4315853533 cites W1986491579 @default.
• W4315853533 cites W1986711353 @default.
• W4315853533 cites W1998347914 @default.
• W4315853533 cites W2001491328 @default.
• W4315853533 cites W2004870223 @default.
• W4315853533 cites W2010415986 @default.
• W4315853533 cites W2014385950 @default.
• W4315853533 cites W2015370039 @default.
• W4315853533 cites W2031950886 @default.
• W4315853533 cites W2035447817 @default.
• W4315853533 cites W2066517842 @default.
• W4315853533 cites W2099540110 @default.
• W4315853533 cites W2099987453 @default.
• W4315853533 cites W2112156060 @default.
• W4315853533 cites W2125928172 @default.
• W4315853533 cites W2169284947 @default.
• W4315853533 cites W2262938430 @default.
• W4315853533 cites W2413335515 @default.
• W4315853533 cites W2583288452 @default.
• W4315853533 cites W2588374458 @default.
• W4315853533 cites W2913267419 @default.
• W4315853533 cites W2972545602 @default.
• W4315853533 cites W2991495895 @default.
• W4315853533 cites W3047702677 @default.
• W4315853533 cites W3127649275 @default.
• W4315853533 cites W4213223693 @default.
• W4315853533 doi "https://doi.org/10.3390/cells12020304" @default.
• W4315853533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36672239" @default.
• W4315853533 hasPublicationYear "2023" @default.
• W4315853533 type Work @default.
• W4315853533 citedByCount "1" @default.
• W4315853533 countsByYear W43158535332023 @default.
• W4315853533 crossrefType "journal-article" @default.
• W4315853533 hasAuthorship W4315853533A5003707560 @default.
• W4315853533 hasAuthorship W4315853533A5010257496 @default.
• W4315853533 hasAuthorship W4315853533A5019795857 @default.
• W4315853533 hasAuthorship W4315853533A5023950095 @default.
• W4315853533 hasAuthorship W4315853533A5029609758 @default.
• W4315853533 hasAuthorship W4315853533A5039303359 @default.
• W4315853533 hasAuthorship W4315853533A5059821769 @default.
• W4315853533 hasAuthorship W4315853533A5064406709 @default.
• W4315853533 hasAuthorship W4315853533A5082089215 @default.
• W4315853533 hasBestOaLocation W43158535331 @default.
• W4315853533 hasConcept C104317684 @default.
• W4315853533 hasConcept C107459253 @default.
• W4315853533 hasConcept C142669718 @default.
• W4315853533 hasConcept C145103041 @default.
• W4315853533 hasConcept C1491633281 @default.
• W4315853533 hasConcept C166703698 @default.
• W4315853533 hasConcept C207701140 @default.
• W4315853533 hasConcept C2993400109 @default.
• W4315853533 hasConcept C51785407 @default.
• W4315853533 hasConcept C54355233 @default.
• W4315853533 hasConcept C67705224 @default.
• W4315853533 hasConcept C77255625 @default.
• W4315853533 hasConcept C86803240 @default.
• W4315853533 hasConcept C95444343 @default.
• W4315853533 hasConceptScore W4315853533C104317684 @default.
• W4315853533 hasConceptScore W4315853533C107459253 @default.
• W4315853533 hasConceptScore W4315853533C142669718 @default.
• W4315853533 hasConceptScore W4315853533C145103041 @default.
• W4315853533 hasConceptScore W4315853533C1491633281 @default.
• W4315853533 hasConceptScore W4315853533C166703698 @default.
• W4315853533 hasConceptScore W4315853533C207701140 @default.
• W4315853533 hasConceptScore W4315853533C2993400109 @default.
• W4315853533 hasConceptScore W4315853533C51785407 @default.
• W4315853533 hasConceptScore W4315853533C54355233 @default.
• W4315853533 hasConceptScore W4315853533C67705224 @default.
• W4315853533 hasConceptScore W4315853533C77255625 @default.
• W4315853533 hasConceptScore W4315853533C86803240 @default.
• W4315853533 hasConceptScore W4315853533C95444343 @default.
• W4315853533 hasFunder F4320321079 @default.
• W4315853533 hasIssue "2" @default.
• W4315853533 hasLocation W43158535331 @default.
• W4315853533 hasLocation W43158535332 @default.
• W4315853533 hasLocation W43158535333 @default.
• W4315853533 hasOpenAccess W4315853533 @default.
• W4315853533 hasPrimaryLocation W43158535331 @default.

• next