FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4315865833> ?p ?o ?g. }

• W4315865833 abstract "Plasmodium falciparum (Pf) is the leading protozoan causing malaria, the most devastating parasitic disease. To ensure transmission, a small subset of Pf parasites differentiate into the sexual forms (gametocytes). Since the abundance of these essential parasitic forms is extremely low within the human host, little is currently known about the molecular regulation of their sexual differentiation, highlighting the need to develop tools to investigate Pf gene expression during this fundamental mechanism.We developed a high-throughput quantitative Reverse-Transcription PCR (RT-qPCR) platform to robustly monitor Pf transcriptional patterns, in particular, systematically profiling the transcriptional pattern of a large panel of gametocyte-related genes (GRG). Initially, we evaluated the technical performance of the systematic RT-qPCR platform to ensure it complies with the accepted quality standards for: (i) RNA extraction, (ii) cDNA synthesis and (iii) evaluation of gene expression through RT-qPCR. We then used this approach to monitor alterations in gene expression of a panel of GRG upon treatment with gametocytogenesis regulators.We thoroughly elucidated GRG expression profiles under treatment with the antimalarial drug dihydroartemisinin (DHA) or the metabolite choline over the course of a Pf blood cycle (48 h). We demonstrate that both significantly alter the expression pattern of PfAP2-G, the gametocytogenesis master regulator. However, they also markedly modify the developmental rate of the parasites and thus might bias the mRNA expression. Additionally, we screened the effect of the metabolites lactate and kynurenic acid, abundant in severe malaria, as potential regulators of gametocytogenesis.Our data demonstrate that the high-throughput RT-qPCR method enables studying the immediate transcriptional response initiating gametocytogenesis of the parasites from a very low volume of malaria-infected RBC samples. The obtained data expand the current knowledge of the initial alterations in mRNA profiles of GRG upon treatment with reported regulators. In addition, using this method emphasizes that asexual parasite stage composition is a crucial element that must be considered when interpreting changes in GRG expression by RT-qPCR, specifically when screening for novel compounds that could regulate Pf sexual differentiation." @default.
• W4315865833 created "2023-01-13" @default.
• W4315865833 creator A5010478613 @default.
• W4315865833 creator A5010786873 @default.
• W4315865833 creator A5027909413 @default.
• W4315865833 creator A5030750309 @default.
• W4315865833 creator A5035536686 @default.
• W4315865833 creator A5044556935 @default.
• W4315865833 creator A5051396163 @default.
• W4315865833 creator A5057721040 @default.
• W4315865833 creator A5073024960 @default.
• W4315865833 creator A5076665919 @default.
• W4315865833 creator A5081305138 @default.
• W4315865833 creator A5084592736 @default.
• W4315865833 creator A5087526855 @default.
• W4315865833 date "2023-01-13" @default.
• W4315865833 modified "2023-09-25" @default.
• W4315865833 title "High-throughput analysis of the transcriptional patterns of sexual genes in malaria" @default.
• W4315865833 cites W1501135527 @default.
• W4315865833 cites W1533689894 @default.
• W4315865833 cites W1576735252 @default.
• W4315865833 cites W1696199194 @default.
• W4315865833 cites W1894135691 @default.
• W4315865833 cites W1969503178 @default.
• W4315865833 cites W1969590352 @default.
• W4315865833 cites W1974091218 @default.
• W4315865833 cites W1978350920 @default.
• W4315865833 cites W1982340876 @default.
• W4315865833 cites W1983724086 @default.
• W4315865833 cites W1984204266 @default.
• W4315865833 cites W1985479044 @default.
• W4315865833 cites W2003424868 @default.
• W4315865833 cites W2011524717 @default.
• W4315865833 cites W2012614126 @default.
• W4315865833 cites W2020251171 @default.
• W4315865833 cites W2021868082 @default.
• W4315865833 cites W2041600775 @default.
• W4315865833 cites W2047734632 @default.
• W4315865833 cites W2047849307 @default.
• W4315865833 cites W2048671997 @default.
• W4315865833 cites W2050350178 @default.
• W4315865833 cites W2056732517 @default.
• W4315865833 cites W2068585157 @default.
• W4315865833 cites W2078096892 @default.
• W4315865833 cites W2082625728 @default.
• W4315865833 cites W2083620174 @default.
• W4315865833 cites W2086287990 @default.
• W4315865833 cites W2093478166 @default.
• W4315865833 cites W2094381221 @default.
• W4315865833 cites W2096496718 @default.
• W4315865833 cites W2100918826 @default.
• W4315865833 cites W2102644922 @default.
• W4315865833 cites W2112047656 @default.
• W4315865833 cites W2129783376 @default.
• W4315865833 cites W2134812217 @default.
• W4315865833 cites W2152791364 @default.
• W4315865833 cites W2155180028 @default.
• W4315865833 cites W2158005619 @default.
• W4315865833 cites W2160568395 @default.
• W4315865833 cites W2160751388 @default.
• W4315865833 cites W2165151268 @default.
• W4315865833 cites W2168420558 @default.
• W4315865833 cites W2168928027 @default.
• W4315865833 cites W2238316440 @default.
• W4315865833 cites W2337669408 @default.
• W4315865833 cites W2415685063 @default.
• W4315865833 cites W2415772202 @default.
• W4315865833 cites W2439364843 @default.
• W4315865833 cites W2462728466 @default.
• W4315865833 cites W2512605033 @default.
• W4315865833 cites W2562857250 @default.
• W4315865833 cites W2576089136 @default.
• W4315865833 cites W2608921790 @default.
• W4315865833 cites W2741777007 @default.
• W4315865833 cites W2759621766 @default.
• W4315865833 cites W2768045943 @default.
• W4315865833 cites W2774233090 @default.
• W4315865833 cites W2790348370 @default.
• W4315865833 cites W2792835715 @default.
• W4315865833 cites W2856061632 @default.
• W4315865833 cites W2884641668 @default.
• W4315865833 cites W2895760106 @default.
• W4315865833 cites W2901933108 @default.
• W4315865833 cites W2945402889 @default.
• W4315865833 cites W2947943269 @default.
• W4315865833 cites W2979485895 @default.
• W4315865833 cites W2984852552 @default.
• W4315865833 cites W2987014493 @default.
• W4315865833 cites W2994952652 @default.
• W4315865833 cites W3011437041 @default.
• W4315865833 cites W3034479438 @default.
• W4315865833 cites W3092001407 @default.
• W4315865833 cites W3093092539 @default.
• W4315865833 cites W3094254745 @default.
• W4315865833 cites W3108452664 @default.
• W4315865833 cites W3121014404 @default.
• W4315865833 cites W3184624734 @default.
• W4315865833 cites W38661849 @default.
• W4315865833 cites W4210461847 @default.
• W4315865833 cites W4211051628 @default.

• next