FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4316015774> ?p ?o ?g. }

• W4316015774 abstract "To explore the correlation between tumor endothelial marker 1 (TEM1) and matrix metalloproteinase 2 (MMP-2) in uterine sarcoma and their roles in the progression of uterine sarcoma.Uterine leiomyosarcoma (uLMS, n = 25) and uterine leiomyoma (n = 25) specimens were collected from a total of 50 patients. Immunohistochemistry assay was conducted to determine the expression of TEM1, MMP-2 and MMP-9. TEM1 over expression (hTEM1) and low expression (shRNA-TEM1) MES-SA cell lines were established as in vitro uterine sarcoma models. MMP-2 mRNA, protein expression and enzymatic activity were verified using qPCR, Western blot and gelatin zymography respectively. MMP-2 expression was downregulated using MMP-2 siRNA in hTEM1 MES-SA cells to better study the role of MMP-2. The invasive and migratory capacities of hTEM1, shRNA-TEM1, and hTEM1 treated with MMP-2 siRNA MES-SA cells were determined using transwell assays. Extracellular matrix (ECM) remodeling mediated by TEM1 was examined using cell-ECM adhesion and fluorescent gelatin-ECM degradation assays. The immunofluorescence of F-actin was examined to analyze the formation of invadopodia. Subcutaneous and intraperitoneal xenografts were established to validate the role of TEM1 in promoting uterine sarcoma metastasis.TEM1 and MMP-2 were expressed in 92% (n = 23) and 88% (n = 22) of uterine leiomyosarcoma specimens, respectively. Both TEM1 and MMP-2 were highly expressed in 100% (n = 17) of high stage (III-IV) uterine leiomyosarcoma specimens. In addition, TEM1 expression was positively correlated with MMP-2 expression in uterine leiomyosarcoma. The successful establishment of in vitro uterine sarcoma models was confirmed with qPCR and Western blotting tests. TEM1 promoted the invasion and metastasis of uterine sarcoma in vivo and in vitro. MMP-2 expression and activity were up-regulated in hTEM1 cells but down-regulated in shRNA-TEM1 cells. Importantly, MMP-2 knockdown impaired the invasive and migratory capacity of hTEM1 cells. TEM1 promoted ECM remodeling by increasing cell-ECM adhesion and ECM degradation. TEM1 overexpression also induced the formation of invadopodia.TEM1 was co-expressed and positively correlated with MMP-2 in uterine leiomyosarcoma specimens. In addition, both TEM1 and MMP-2 were associated with tumor development. TEM1 promoted uterine sarcoma progression by regulating MMP-2 activity and ECM remodeling." @default.
• W4316015774 created "2023-01-14" @default.
• W4316015774 creator A5048729781 @default.
• W4316015774 creator A5053290799 @default.
• W4316015774 creator A5063048411 @default.
• W4316015774 creator A5081970545 @default.
• W4316015774 creator A5082946818 @default.
• W4316015774 creator A5087890346 @default.
• W4316015774 date "2023-01-13" @default.
• W4316015774 modified "2023-10-14" @default.
• W4316015774 title "TEM1 up-regulates MMP-2 and promotes ECM remodeling for facilitating invasion and migration of uterine sarcoma" @default.
• W4316015774 cites W1929233845 @default.
• W4316015774 cites W1939992014 @default.
• W4316015774 cites W1965498876 @default.
• W4316015774 cites W1978818883 @default.
• W4316015774 cites W1981641775 @default.
• W4316015774 cites W1993076894 @default.
• W4316015774 cites W2006335846 @default.
• W4316015774 cites W2006793254 @default.
• W4316015774 cites W2012809609 @default.
• W4316015774 cites W2014364531 @default.
• W4316015774 cites W2024045219 @default.
• W4316015774 cites W2031391627 @default.
• W4316015774 cites W2032373323 @default.
• W4316015774 cites W2076022365 @default.
• W4316015774 cites W2083447892 @default.
• W4316015774 cites W2084054324 @default.
• W4316015774 cites W2086564653 @default.
• W4316015774 cites W2090716194 @default.
• W4316015774 cites W2091072508 @default.
• W4316015774 cites W2096820349 @default.
• W4316015774 cites W2100164970 @default.
• W4316015774 cites W2100426671 @default.
• W4316015774 cites W2115636010 @default.
• W4316015774 cites W2125743967 @default.
• W4316015774 cites W2127236416 @default.
• W4316015774 cites W2129985201 @default.
• W4316015774 cites W2131314830 @default.
• W4316015774 cites W2132566055 @default.
• W4316015774 cites W2139906829 @default.
• W4316015774 cites W2159482492 @default.
• W4316015774 cites W2164236122 @default.
• W4316015774 cites W2167067909 @default.
• W4316015774 cites W2217398072 @default.
• W4316015774 cites W2325389150 @default.
• W4316015774 cites W2387879652 @default.
• W4316015774 cites W2589139101 @default.
• W4316015774 cites W2737769268 @default.
• W4316015774 cites W2747662215 @default.
• W4316015774 cites W2766758038 @default.
• W4316015774 cites W2781504446 @default.
• W4316015774 cites W2781624907 @default.
• W4316015774 cites W2867022353 @default.
• W4316015774 cites W2897759832 @default.
• W4316015774 cites W2943604071 @default.
• W4316015774 cites W2955269731 @default.
• W4316015774 cites W2968818721 @default.
• W4316015774 cites W2991969456 @default.
• W4316015774 cites W2992640926 @default.
• W4316015774 cites W2996059929 @default.
• W4316015774 cites W2996555196 @default.
• W4316015774 cites W3007816118 @default.
• W4316015774 cites W3008903559 @default.
• W4316015774 cites W3029784168 @default.
• W4316015774 cites W3037667061 @default.
• W4316015774 cites W3038168943 @default.
• W4316015774 cites W3083038341 @default.
• W4316015774 cites W3084946487 @default.
• W4316015774 cites W3092007298 @default.
• W4316015774 cites W3093970028 @default.
• W4316015774 cites W3130251034 @default.
• W4316015774 cites W3135900406 @default.
• W4316015774 cites W3139030537 @default.
• W4316015774 cites W3171545823 @default.
• W4316015774 cites W3192563092 @default.
• W4316015774 cites W3202021422 @default.
• W4316015774 cites W3208789019 @default.
• W4316015774 cites W4200077518 @default.
• W4316015774 cites W4206916604 @default.
• W4316015774 cites W4210547813 @default.
• W4316015774 cites W4223441634 @default.
• W4316015774 cites W4292491750 @default.
• W4316015774 cites W49724973 @default.
• W4316015774 cites W75660270 @default.
• W4316015774 doi "https://doi.org/10.1007/s12672-023-00613-6" @default.
• W4316015774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36639546" @default.
• W4316015774 hasPublicationYear "2023" @default.
• W4316015774 type Work @default.
• W4316015774 citedByCount "0" @default.
• W4316015774 crossrefType "journal-article" @default.
• W4316015774 hasAuthorship W4316015774A5048729781 @default.
• W4316015774 hasAuthorship W4316015774A5053290799 @default.
• W4316015774 hasAuthorship W4316015774A5063048411 @default.
• W4316015774 hasAuthorship W4316015774A5081970545 @default.
• W4316015774 hasAuthorship W4316015774A5082946818 @default.
• W4316015774 hasAuthorship W4316015774A5087890346 @default.
• W4316015774 hasBestOaLocation W43160157741 @default.
• W4316015774 hasConcept C104317684 @default.
• W4316015774 hasConcept C109523444 @default.
• W4316015774 hasConcept C121608353 @default.

• next