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• W4316468729 abstract "Advanced fluorescence imaging techniques such as single-molecule localization microscopy (SMLM) fundamentally rely on the photophysical behavior of the employed fluorophores. This behavior is generally complex and impacts data quality in a subtle manner. A simulation software named Single-Molecule Imaging Simulator (SMIS) is introduced that simulates a widefield microscope and incorporates fluorophores with their spectral and photophysical properties. With SMIS, data collection schemes combining 3D, multicolor, single-particle-tracking or quantitative SMLM can be implemented. The influence of advanced fluorophore characteristics, imaging conditions, and environmental parameters can be evaluated, facilitating the design of real experiments and their proper interpretation." @default.
• W4316468729 created "2023-01-16" @default.
• W4316468729 creator A5010878167 @default.
• W4316468729 date "2023-01-16" @default.
• W4316468729 modified "2023-10-14" @default.
• W4316468729 title "Single molecule imaging simulations with advanced fluorophore photophysics" @default.
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• W4316468729 doi "https://doi.org/10.1038/s42003-023-04432-x" @default.
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