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- W4316496679 abstract "It is of great significance to implement early risk assessment of diabetes using point-of-care testing (POCT) strategies, particularly in the resource-limited settings. Here we proposed a low-cost portable microfluidic POCT chemiluminometer for the determination of adiponectin, an effective biomarker of diabetes. The determination is based on the immunoassay on a centrifugal microfluidic chip made of polycarbonate with 40 parallel detection microchannels. Centrifugation assisted washing processes made the entire immunoassay workflow semi-automatic and easy to perform. The chemiluminescence signal was recorded by a specially designed portable chemiluminometer. The whole set of the device costs less than $600 and weighs only 0.5 kg, which is suitable for POCT use. A limit of detection of 2.7 ng/ml was achieved with a linear response up to 70.5 ng/ml (R2 = 0.975) for serum adiponectin, covering the physiological blood adiponectin levels. Successful determination of adiponectin in six clinical specimens was demonstrated by a comparison with standard enzyme-linked immunosorbent assay." @default.
- W4316496679 created "2023-01-16" @default.
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- W4316496679 date "2023-04-01" @default.
- W4316496679 modified "2023-10-02" @default.
- W4316496679 title "A low-cost portable microfluidic chemiluminometer for point-of-care testing of adiponectin for early risk assessment of diabetes" @default.
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- W4316496679 doi "https://doi.org/10.1016/j.snb.2023.133384" @default.
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