Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317107788> ?p ?o ?g. }
- W4317107788 abstract "The wild species field cress ( Lepidium campestre ) has the potential to become a novel cover and oilseed crop for the Nordic climate. Its seed oil is however currently unsuitable for most food, feed, and industrial applications, due to the high contents of polyunsaturated fatty acids (PUFAs) and erucic acid (C22:1). As the biosynthesis of these undesirable fatty acids is controlled by a few well-known major dominant genes, knockout of these genes using CRISPR/Cas9 would thus be more effective in improving the seed oil quality. In order to increase the level of the desirable oleic acid (C18:1), and reduce the contents of PUFAs and C22:1, we targeted three important genes FATTY ACID ELONGASE1 ( FAE1 ), FATTY ACID DESATURASE2 ( FAD2 ), and REDUCED OLEATE DESATURASE1 (ROD1 ) using a protoplast-based CRISPR/Cas9 gene knockout system. By knocking out FAE1 , we obtained a mutated line with almost no C22:1, but an increase in C18:1 to 30% compared with 13% in the wild type. Knocking out ROD1 resulted in an increase of C18:1 to 23%, and a moderate, but significant, reduction of PUFAs. Knockout of FAD2 , in combination with heterozygous FAE1fae1 genotype, resulted in mutated lines with up to 66% C18:1, very low contents of PUFAs, and a significant reduction of C22:1. Our results clearly show the potential of CRISPR/Cas9 for rapid trait improvement of field cress which would speed up its domestication process. The mutated lines produced in this study can be used for further breeding to develop field cress into a viable crop." @default.
- W4317107788 created "2023-01-18" @default.
- W4317107788 creator A5007953104 @default.
- W4317107788 creator A5038756883 @default.
- W4317107788 creator A5047955322 @default.
- W4317107788 creator A5050308841 @default.
- W4317107788 creator A5054063853 @default.
- W4317107788 creator A5062845778 @default.
- W4317107788 creator A5067081104 @default.
- W4317107788 date "2023-01-18" @default.
- W4317107788 modified "2023-10-16" @default.
- W4317107788 title "Improved fatty acid composition of field cress (Lepidium campestre) by CRISPR/Cas9-mediated genome editing" @default.
- W4317107788 cites W1508162533 @default.
- W4317107788 cites W1838293818 @default.
- W4317107788 cites W1979168325 @default.
- W4317107788 cites W1982802227 @default.
- W4317107788 cites W2016993318 @default.
- W4317107788 cites W2025256487 @default.
- W4317107788 cites W2037411737 @default.
- W4317107788 cites W2051723988 @default.
- W4317107788 cites W2066659636 @default.
- W4317107788 cites W2113493415 @default.
- W4317107788 cites W2114391027 @default.
- W4317107788 cites W2143149790 @default.
- W4317107788 cites W2145782793 @default.
- W4317107788 cites W2151934250 @default.
- W4317107788 cites W2307760237 @default.
- W4317107788 cites W2431409664 @default.
- W4317107788 cites W2528613931 @default.
- W4317107788 cites W2771920161 @default.
- W4317107788 cites W2773816027 @default.
- W4317107788 cites W2793762852 @default.
- W4317107788 cites W2803479625 @default.
- W4317107788 cites W2892968638 @default.
- W4317107788 cites W2893455966 @default.
- W4317107788 cites W2894252291 @default.
- W4317107788 cites W2918418208 @default.
- W4317107788 cites W2943872942 @default.
- W4317107788 cites W2956233309 @default.
- W4317107788 cites W2998971016 @default.
- W4317107788 cites W3003666557 @default.
- W4317107788 cites W3020906279 @default.
- W4317107788 cites W3027165922 @default.
- W4317107788 cites W3158280211 @default.
- W4317107788 cites W3178807673 @default.
- W4317107788 cites W3213713040 @default.
- W4317107788 cites W4210646185 @default.
- W4317107788 cites W4211079033 @default.
- W4317107788 cites W4225256839 @default.
- W4317107788 cites W4293770972 @default.
- W4317107788 cites W2042177576 @default.
- W4317107788 doi "https://doi.org/10.3389/fpls.2023.1076704" @default.
- W4317107788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36755695" @default.
- W4317107788 hasPublicationYear "2023" @default.
- W4317107788 type Work @default.
- W4317107788 citedByCount "1" @default.
- W4317107788 countsByYear W43171077882023 @default.
- W4317107788 crossrefType "journal-article" @default.
- W4317107788 hasAuthorship W4317107788A5007953104 @default.
- W4317107788 hasAuthorship W4317107788A5038756883 @default.
- W4317107788 hasAuthorship W4317107788A5047955322 @default.
- W4317107788 hasAuthorship W4317107788A5050308841 @default.
- W4317107788 hasAuthorship W4317107788A5054063853 @default.
- W4317107788 hasAuthorship W4317107788A5062845778 @default.
- W4317107788 hasAuthorship W4317107788A5067081104 @default.
- W4317107788 hasBestOaLocation W43171077881 @default.
- W4317107788 hasConcept C104317684 @default.
- W4317107788 hasConcept C132455925 @default.
- W4317107788 hasConcept C144501496 @default.
- W4317107788 hasConcept C150903083 @default.
- W4317107788 hasConcept C19038510 @default.
- W4317107788 hasConcept C2777129432 @default.
- W4317107788 hasConcept C2780838596 @default.
- W4317107788 hasConcept C2781403372 @default.
- W4317107788 hasConcept C31903555 @default.
- W4317107788 hasConcept C543025807 @default.
- W4317107788 hasConcept C54355233 @default.
- W4317107788 hasConcept C55493867 @default.
- W4317107788 hasConcept C59822182 @default.
- W4317107788 hasConcept C86803240 @default.
- W4317107788 hasConcept C98108389 @default.
- W4317107788 hasConceptScore W4317107788C104317684 @default.
- W4317107788 hasConceptScore W4317107788C132455925 @default.
- W4317107788 hasConceptScore W4317107788C144501496 @default.
- W4317107788 hasConceptScore W4317107788C150903083 @default.
- W4317107788 hasConceptScore W4317107788C19038510 @default.
- W4317107788 hasConceptScore W4317107788C2777129432 @default.
- W4317107788 hasConceptScore W4317107788C2780838596 @default.
- W4317107788 hasConceptScore W4317107788C2781403372 @default.
- W4317107788 hasConceptScore W4317107788C31903555 @default.
- W4317107788 hasConceptScore W4317107788C543025807 @default.
- W4317107788 hasConceptScore W4317107788C54355233 @default.
- W4317107788 hasConceptScore W4317107788C55493867 @default.
- W4317107788 hasConceptScore W4317107788C59822182 @default.
- W4317107788 hasConceptScore W4317107788C86803240 @default.
- W4317107788 hasConceptScore W4317107788C98108389 @default.
- W4317107788 hasLocation W43171077881 @default.
- W4317107788 hasLocation W43171077882 @default.
- W4317107788 hasLocation W43171077883 @default.
- W4317107788 hasLocation W43171077884 @default.