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- W4317181675 abstract "Background and Aim Exposure to environmental chemicals during development may increase the susceptibility to adiposity/obesity in adolescence and adulthood. Bisphenol S (BPS) has been suspected as an obesogen after perinatal exposure. Previously, we reported adipocyte hypertrophy in the gonadal white adipose tissue (gWAT) of F1 male mice after the prenatal exposure to BPS followed by high-fat diet (HFD) in adulthood. The expression of the mesoderm-specific transcript (Mest) gene is shown to correlate with adipocyte size and adiposity. Our study aimed to investigate the environmentally induced epigenetic modulation, such as DNA methylation changes in Mest, leading to gonadal adiposity of male offspring. Methods The mRNA expression of Mest in the gWAT of F1 male mice (C57BL/6N) was analyzed by reverse transcription-quantitative PCR (RT-qPCR). We performed bisulfite amplicon sequencing (BSAS) analysis to identify the CpG dinucleotides at the differentially methylated region (DMR) of Mest that were associated with adiposity phenotype. The DNA methylation levels of the site-specific CpGs were quantified by targeted bisulfite pyrosequencing. Results The Mest mRNA levels in the gWAT increased in a dose-dependent manner, compared to the BPS-unexposed control. Three CpG loci exhibiting significant differences in DNA methylation were identified through BSAS analysis of the Mest DMR. Consistent with BSAS data, each site-specific CpG at the Mest DMR was found to be differentially methylated among extreme groups with both gWAT/body weight ratio and the Mest expression levels. Conclusions Our study suggests that prenatal BPS and postnatal HFD might induce epigenetic influence, i.e., DNA methylation changes, on the Mest DMR, and consequently contribute to the gonadal adiposity in F1 male mice. Furthermore, the distinct epigenotype of Mest could be utilized as a biomarker of the exposure to potential adipogenic epigenotoxicants. Keywords Bisphenol S, high-fat diet, gonadal adiposity, Mest, epigenetic modulation, DNA methylation" @default.
- W4317181675 created "2023-01-18" @default.
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- W4317181675 date "2022-09-18" @default.
- W4317181675 modified "2023-09-28" @default.
- W4317181675 title "Prenatal Bisphenol S and Postnatal High-Fat Diet Influence DNA Methylation of the Mest Gene in F1 Male Mice" @default.
- W4317181675 doi "https://doi.org/10.1289/isee.2022.p-0977" @default.
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