Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317508766> ?p ?o ?g. }
- W4317508766 endingPage "1964" @default.
- W4317508766 startingPage "1964" @default.
- W4317508766 abstract "Super-enhancers (SEs) regulate gene expressions, which are critical for cell type-identity and tumorigenesis. Although genome wide H3K27ac profiling have revealed the presence of SE-associated genes in gastric cancer (GC), their roles remain unclear. In this study, ChIP-seq and HiChIP-seq experiments revealed mitogen-activated protein kinase 8 (MAP3K8) to be an SE-associated gene with chromosome interactions in Epstein–Barr virus-associated gastric carcinoma (EBVaGC) cells. CRISPRi mediated repression of the MAP3K8 SEs attenuated MAP3K8 expression and EBVaGC cell proliferation. The results were validated by treating EBVaGC cells with bromodomain and the extra-terminal motif (BET) inhibitor, OTX015. Further, functional analysis of MAP3K8 in EBVaGC revealed that silencing MAP3K8 could inhibit the cell proliferation, colony formation, and migration of EBVaGC cells. RNA-seq and pathway analysis indicated that knocking down MAP3K8 obstructed the notch signaling pathway and epithelial-mesenchymal transition (EMT) in EBVaGC cells. Further, analysis of the cancer genome atlas (TCGA) and GSE51575 databases exhibited augmented MAP3K8 expression in gastric cancer and it was found to be inversely correlated with the disease-free progression of GC. Moreover, Spearman’s correlation revealed that MAP3K8 expression was positively correlated with the expressions of notch pathway and EMT related genes, such as, Notch1, Notch2, C-terminal binding protein 2 (CTBP2), alpha smooth muscle actin isotype 2 (ACTA2), transforming growth factor beta receptor 1 (TGFβR1), and snail family transcriptional repressors 1/2 (SNAI1/SNAI2) in GC. Taken together, we are the first to functionally interrogate the mechanism of SE-mediated regulation of MAP3K8 in EBVaGC cell lines." @default.
- W4317508766 created "2023-01-20" @default.
- W4317508766 creator A5003628038 @default.
- W4317508766 creator A5038141734 @default.
- W4317508766 creator A5044067622 @default.
- W4317508766 creator A5052501867 @default.
- W4317508766 creator A5053281064 @default.
- W4317508766 creator A5061678538 @default.
- W4317508766 date "2023-01-19" @default.
- W4317508766 modified "2023-10-14" @default.
- W4317508766 title "Epigenetic Regulation of MAP3K8 in EBV-Associated Gastric Carcinoma" @default.
- W4317508766 cites W190848849 @default.
- W4317508766 cites W1909477108 @default.
- W4317508766 cites W1974450480 @default.
- W4317508766 cites W1988597072 @default.
- W4317508766 cites W2032261877 @default.
- W4317508766 cites W2035208208 @default.
- W4317508766 cites W2056832257 @default.
- W4317508766 cites W2070021921 @default.
- W4317508766 cites W2071963408 @default.
- W4317508766 cites W2073799618 @default.
- W4317508766 cites W2088928744 @default.
- W4317508766 cites W2117247812 @default.
- W4317508766 cites W2120817191 @default.
- W4317508766 cites W2124655831 @default.
- W4317508766 cites W2126547130 @default.
- W4317508766 cites W2136018911 @default.
- W4317508766 cites W2139139191 @default.
- W4317508766 cites W2141674733 @default.
- W4317508766 cites W2154700978 @default.
- W4317508766 cites W2154861644 @default.
- W4317508766 cites W2160307341 @default.
- W4317508766 cites W2286136774 @default.
- W4317508766 cites W2288117658 @default.
- W4317508766 cites W2289394887 @default.
- W4317508766 cites W2324000374 @default.
- W4317508766 cites W2507897093 @default.
- W4317508766 cites W2515020216 @default.
- W4317508766 cites W2520148131 @default.
- W4317508766 cites W2524890297 @default.
- W4317508766 cites W2587899062 @default.
- W4317508766 cites W2591297368 @default.
- W4317508766 cites W2604329262 @default.
- W4317508766 cites W2606230468 @default.
- W4317508766 cites W2766570385 @default.
- W4317508766 cites W2768302346 @default.
- W4317508766 cites W2782103295 @default.
- W4317508766 cites W2783337717 @default.
- W4317508766 cites W2787553551 @default.
- W4317508766 cites W2792191537 @default.
- W4317508766 cites W2891879783 @default.
- W4317508766 cites W2893324794 @default.
- W4317508766 cites W2903072518 @default.
- W4317508766 cites W2912868289 @default.
- W4317508766 cites W2916858557 @default.
- W4317508766 cites W2919716556 @default.
- W4317508766 cites W2921841818 @default.
- W4317508766 cites W2940498808 @default.
- W4317508766 cites W2966019641 @default.
- W4317508766 cites W2973963265 @default.
- W4317508766 cites W2974304113 @default.
- W4317508766 cites W2982051641 @default.
- W4317508766 cites W2988158628 @default.
- W4317508766 cites W3000737316 @default.
- W4317508766 cites W3005196658 @default.
- W4317508766 cites W3006667348 @default.
- W4317508766 cites W3016044799 @default.
- W4317508766 cites W3020208951 @default.
- W4317508766 cites W3029382550 @default.
- W4317508766 cites W3029679669 @default.
- W4317508766 cites W3033366394 @default.
- W4317508766 cites W3044410956 @default.
- W4317508766 cites W3044762217 @default.
- W4317508766 cites W3045133718 @default.
- W4317508766 cites W3045182026 @default.
- W4317508766 cites W3047691886 @default.
- W4317508766 cites W3048653545 @default.
- W4317508766 cites W3048808000 @default.
- W4317508766 cites W3049642946 @default.
- W4317508766 cites W3095863449 @default.
- W4317508766 cites W3097206973 @default.
- W4317508766 cites W3110314789 @default.
- W4317508766 cites W3112303823 @default.
- W4317508766 cites W3128646645 @default.
- W4317508766 cites W3138977384 @default.
- W4317508766 cites W3162889843 @default.
- W4317508766 cites W3163973087 @default.
- W4317508766 cites W3183469219 @default.
- W4317508766 cites W3184006782 @default.
- W4317508766 cites W3199200626 @default.
- W4317508766 cites W3199390659 @default.
- W4317508766 cites W3215804303 @default.
- W4317508766 cites W4200308108 @default.
- W4317508766 cites W4210944285 @default.
- W4317508766 cites W4223591496 @default.
- W4317508766 cites W4291134525 @default.
- W4317508766 doi "https://doi.org/10.3390/ijms24031964" @default.
- W4317508766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36768307" @default.