Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317565539> ?p ?o ?g. }
- W4317565539 abstract "Antibody gold nanoparticle conjugates as recognition elements are essential for the overall performance of lateral flow assays. When immobilizing antibodies on gold nanoparticles, the challenge is to prevent aggregation and to ensure that the antibodies are correctly oriented so that they remain functional and their paratopes remain accessible. There are many methods available, and it is difficult to decide which one to use. To help selecting the most appropriate conjugate production method, different synthetic routes of binding antibodies to gold nanoparticles are systematically investigated for the purpose of a quantitative lateral flow test for small molecules. The direct comparison of different conjugate syntheses shows how to select a suitable conjugate for a lateral flow assay. The syntheses examined are direct adsorption of antibody, direct adsorption of reduced antibody, covalent binding to polyethylene glycol linker, and binding via biotin-streptavidin interaction. The conjugates are characterized using UV-Vis spectroscopy and dynamic light scattering to determine their stability. Their performance on structured lateral flow test strips is examined using calibrations for different amitriptyline concentrations. It was shown that the best conjugate for quantification of amitriptyline was realized by direct adsorption of an UV-light irradiated antibody to gold nanoparticles. The methods employed can serve as a guide for selecting the most appropriate conjugate for an application and enhance the performance of lateral flow assays." @default.
- W4317565539 created "2023-01-21" @default.
- W4317565539 creator A5041701593 @default.
- W4317565539 creator A5062465009 @default.
- W4317565539 creator A5063515816 @default.
- W4317565539 creator A5075233840 @default.
- W4317565539 creator A5089762713 @default.
- W4317565539 creator A5090047168 @default.
- W4317565539 date "2023-01-20" @default.
- W4317565539 modified "2023-10-14" @default.
- W4317565539 title "Tools to compare antibody gold nanoparticle conjugates for a small molecule immunoassay" @default.
- W4317565539 cites W1530276086 @default.
- W4317565539 cites W1971925621 @default.
- W4317565539 cites W1972371845 @default.
- W4317565539 cites W1995801194 @default.
- W4317565539 cites W2019239850 @default.
- W4317565539 cites W2038520392 @default.
- W4317565539 cites W2040085202 @default.
- W4317565539 cites W2044440155 @default.
- W4317565539 cites W2046371751 @default.
- W4317565539 cites W2051654117 @default.
- W4317565539 cites W2056066120 @default.
- W4317565539 cites W2067830052 @default.
- W4317565539 cites W2078211790 @default.
- W4317565539 cites W2087630244 @default.
- W4317565539 cites W2105613624 @default.
- W4317565539 cites W2130592661 @default.
- W4317565539 cites W2133059825 @default.
- W4317565539 cites W2156386692 @default.
- W4317565539 cites W2237562241 @default.
- W4317565539 cites W2292973138 @default.
- W4317565539 cites W2330137314 @default.
- W4317565539 cites W2330754237 @default.
- W4317565539 cites W2417013710 @default.
- W4317565539 cites W2475976299 @default.
- W4317565539 cites W2615851015 @default.
- W4317565539 cites W2753966476 @default.
- W4317565539 cites W2762424071 @default.
- W4317565539 cites W2767195307 @default.
- W4317565539 cites W2807299990 @default.
- W4317565539 cites W2891887198 @default.
- W4317565539 cites W2922485383 @default.
- W4317565539 cites W2957813057 @default.
- W4317565539 cites W2965618763 @default.
- W4317565539 cites W2980268803 @default.
- W4317565539 cites W3094213759 @default.
- W4317565539 cites W3103240054 @default.
- W4317565539 cites W3129331088 @default.
- W4317565539 cites W3197387481 @default.
- W4317565539 doi "https://doi.org/10.1007/s00604-023-05637-x" @default.
- W4317565539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36662292" @default.
- W4317565539 hasPublicationYear "2023" @default.
- W4317565539 type Work @default.
- W4317565539 citedByCount "2" @default.
- W4317565539 crossrefType "journal-article" @default.
- W4317565539 hasAuthorship W4317565539A5041701593 @default.
- W4317565539 hasAuthorship W4317565539A5062465009 @default.
- W4317565539 hasAuthorship W4317565539A5063515816 @default.
- W4317565539 hasAuthorship W4317565539A5075233840 @default.
- W4317565539 hasAuthorship W4317565539A5089762713 @default.
- W4317565539 hasAuthorship W4317565539A5090047168 @default.
- W4317565539 hasBestOaLocation W43175655391 @default.
- W4317565539 hasConcept C104819515 @default.
- W4317565539 hasConcept C111919701 @default.
- W4317565539 hasConcept C134306372 @default.
- W4317565539 hasConcept C150394285 @default.
- W4317565539 hasConcept C155672457 @default.
- W4317565539 hasConcept C159654299 @default.
- W4317565539 hasConcept C171250308 @default.
- W4317565539 hasConcept C178790620 @default.
- W4317565539 hasConcept C185592680 @default.
- W4317565539 hasConcept C192562407 @default.
- W4317565539 hasConcept C197336794 @default.
- W4317565539 hasConcept C203014093 @default.
- W4317565539 hasConcept C204628709 @default.
- W4317565539 hasConcept C21951064 @default.
- W4317565539 hasConcept C2776190903 @default.
- W4317565539 hasConcept C2776964284 @default.
- W4317565539 hasConcept C2778204606 @default.
- W4317565539 hasConcept C2780420688 @default.
- W4317565539 hasConcept C2780557392 @default.
- W4317565539 hasConcept C33923547 @default.
- W4317565539 hasConcept C41008148 @default.
- W4317565539 hasConcept C43617362 @default.
- W4317565539 hasConcept C55493867 @default.
- W4317565539 hasConcept C86803240 @default.
- W4317565539 hasConceptScore W4317565539C104819515 @default.
- W4317565539 hasConceptScore W4317565539C111919701 @default.
- W4317565539 hasConceptScore W4317565539C134306372 @default.
- W4317565539 hasConceptScore W4317565539C150394285 @default.
- W4317565539 hasConceptScore W4317565539C155672457 @default.
- W4317565539 hasConceptScore W4317565539C159654299 @default.
- W4317565539 hasConceptScore W4317565539C171250308 @default.
- W4317565539 hasConceptScore W4317565539C178790620 @default.
- W4317565539 hasConceptScore W4317565539C185592680 @default.
- W4317565539 hasConceptScore W4317565539C192562407 @default.
- W4317565539 hasConceptScore W4317565539C197336794 @default.
- W4317565539 hasConceptScore W4317565539C203014093 @default.
- W4317565539 hasConceptScore W4317565539C204628709 @default.
- W4317565539 hasConceptScore W4317565539C21951064 @default.