FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317666167> ?p ?o ?g. }

• W4317666167 endingPage "130313" @default.
• W4317666167 startingPage "130313" @default.
• W4317666167 abstract "Calmodulin (CaM) modulates the activity of several proteins that play a key role in excitation-contraction coupling (ECC). In cardiac muscle, the major binding partner of CaM is the type-2 ryanodine receptor (RyR2) and altered CaM binding contributes to defects in sarcoplasmic reticulum (SR) calcium (Ca2+) release. Many genetic studies have reported a series of CaM missense mutations in patients with a history of severe arrhythmogenic cardiac disorders. In the present study, we generated four missense CaM mutants (CaMN98I, CaMD132E, CaMD134H and CaMQ136P) and we used a CaM-RyR2 co-immunoprecipitation and a [3H]ryanodine binding assay to directly compare the relative RyR2-binding of wild type and mutant CaM proteins and to investigate the functional effects of these CaM mutations on RyR2 activity. Furthermore, isothermal titration calorimetry (ITC) experiments were performed to investigate and compare the interactions of the wild-type and mutant CaM proteins with various synthetic peptides located in the well-established RyR2 CaM-binding region (3584-3602aa), as well as another CaM-binding region (4255-4271aa) of human RyR2. Our data revealed that all four CaM mutants displayed dramatically reduced RyR2 interaction and defective modulation of [3H]ryanodine binding to RyR2, regardless of LQTS or CPVT association. Moreover, our isothermal titration calorimetry ITC data suggest that RyR2 3584-3602aa and 4255-4271aa regions interact with significant affinity with wild-type CaM, in the presence and absence of Ca2+, two regions that might contribute to a putative intra-subunit CaM-binding pocket. In contrast, screening the interaction of the four arrhythmogenic CaM mutants with two synthetic peptides that correspond to these RyR2 regions, revealed disparate binding properties and signifying differential mechanisms that contribute to reduced RyR2 association." @default.
• W4317666167 created "2023-01-21" @default.
• W4317666167 creator A5016435169 @default.
• W4317666167 creator A5019791297 @default.
• W4317666167 creator A5027719937 @default.
• W4317666167 creator A5030278478 @default.
• W4317666167 creator A5031873153 @default.
• W4317666167 creator A5032431784 @default.
• W4317666167 creator A5032504283 @default.
• W4317666167 creator A5038294065 @default.
• W4317666167 creator A5045507434 @default.
• W4317666167 creator A5058899046 @default.
• W4317666167 creator A5073107235 @default.
• W4317666167 date "2023-04-01" @default.
• W4317666167 modified "2023-10-14" @default.
• W4317666167 title "Life-threatening arrhythmogenic CaM mutations disrupt CaM binding to a distinct RyR2 CaM-binding pocket" @default.
• W4317666167 cites W1509170501 @default.
• W4317666167 cites W1544043865 @default.
• W4317666167 cites W1845756101 @default.
• W4317666167 cites W1893636323 @default.
• W4317666167 cites W1993320492 @default.
• W4317666167 cites W2012544852 @default.
• W4317666167 cites W2020664968 @default.
• W4317666167 cites W2027380922 @default.
• W4317666167 cites W2031215987 @default.
• W4317666167 cites W2031697021 @default.
• W4317666167 cites W2056342772 @default.
• W4317666167 cites W2061095840 @default.
• W4317666167 cites W2063557153 @default.
• W4317666167 cites W2067686089 @default.
• W4317666167 cites W2072271663 @default.
• W4317666167 cites W2080437238 @default.
• W4317666167 cites W2100702361 @default.
• W4317666167 cites W2100733539 @default.
• W4317666167 cites W2112338272 @default.
• W4317666167 cites W2115439195 @default.
• W4317666167 cites W2115812446 @default.
• W4317666167 cites W2130124702 @default.
• W4317666167 cites W2130401575 @default.
• W4317666167 cites W2139214355 @default.
• W4317666167 cites W2312494660 @default.
• W4317666167 cites W2521310122 @default.
• W4317666167 cites W2560369188 @default.
• W4317666167 cites W2898082354 @default.
• W4317666167 cites W2905518644 @default.
• W4317666167 cites W2931683611 @default.
• W4317666167 cites W2951469514 @default.
• W4317666167 cites W2990866452 @default.
• W4317666167 cites W3004666955 @default.
• W4317666167 cites W3138242208 @default.
• W4317666167 cites W3200156731 @default.
• W4317666167 cites W4220903200 @default.
• W4317666167 cites W622186707 @default.
• W4317666167 doi "https://doi.org/10.1016/j.bbagen.2023.130313" @default.
• W4317666167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36693454" @default.
• W4317666167 hasPublicationYear "2023" @default.
• W4317666167 type Work @default.
• W4317666167 citedByCount "0" @default.
• W4317666167 crossrefType "journal-article" @default.
• W4317666167 hasAuthorship W4317666167A5016435169 @default.
• W4317666167 hasAuthorship W4317666167A5019791297 @default.
• W4317666167 hasAuthorship W4317666167A5027719937 @default.
• W4317666167 hasAuthorship W4317666167A5030278478 @default.
• W4317666167 hasAuthorship W4317666167A5031873153 @default.
• W4317666167 hasAuthorship W4317666167A5032431784 @default.
• W4317666167 hasAuthorship W4317666167A5032504283 @default.
• W4317666167 hasAuthorship W4317666167A5038294065 @default.
• W4317666167 hasAuthorship W4317666167A5045507434 @default.
• W4317666167 hasAuthorship W4317666167A5058899046 @default.
• W4317666167 hasAuthorship W4317666167A5073107235 @default.
• W4317666167 hasBestOaLocation W43176661671 @default.
• W4317666167 hasConcept C104317684 @default.
• W4317666167 hasConcept C113217602 @default.
• W4317666167 hasConcept C143065580 @default.
• W4317666167 hasConcept C156911925 @default.
• W4317666167 hasConcept C158617107 @default.
• W4317666167 hasConcept C181199279 @default.
• W4317666167 hasConcept C185592680 @default.
• W4317666167 hasConcept C190712762 @default.
• W4317666167 hasConcept C29688787 @default.
• W4317666167 hasConcept C501734568 @default.
• W4317666167 hasConcept C55493867 @default.
• W4317666167 hasConcept C75563809 @default.
• W4317666167 hasConcept C86803240 @default.
• W4317666167 hasConcept C95444343 @default.
• W4317666167 hasConceptScore W4317666167C104317684 @default.
• W4317666167 hasConceptScore W4317666167C113217602 @default.
• W4317666167 hasConceptScore W4317666167C143065580 @default.
• W4317666167 hasConceptScore W4317666167C156911925 @default.
• W4317666167 hasConceptScore W4317666167C158617107 @default.
• W4317666167 hasConceptScore W4317666167C181199279 @default.
• W4317666167 hasConceptScore W4317666167C185592680 @default.
• W4317666167 hasConceptScore W4317666167C190712762 @default.
• W4317666167 hasConceptScore W4317666167C29688787 @default.
• W4317666167 hasConceptScore W4317666167C501734568 @default.
• W4317666167 hasConceptScore W4317666167C55493867 @default.
• W4317666167 hasConceptScore W4317666167C75563809 @default.
• W4317666167 hasConceptScore W4317666167C86803240 @default.

• next