Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317676017> ?p ?o ?g. }
- W4317676017 endingPage "143" @default.
- W4317676017 startingPage "130" @default.
- W4317676017 abstract "The antibody BCF2 generated against the mammal-specific toxin Cn2 of the scorpion Centruroides noxius Hoffmann neutralizes the effect of both the toxin and the venom. We cloned and sequenced the genes coding for the Fv fragment of BCF2. A three-dimensional (3D) model of the Fv fragment was generated using a knowledge-based approach. Furthermore, a 3D model of the complex Cn2–BCF2 was built using the nuclear magnetic resonance (NMR) structure of Cn2 and experimental results on a putative epitope region around the N and C termini. The initial complex conformations were submitted to a new refinement procedure of rigid-body energy minimization combined with flexible-side-chain molecular dynamics. The final complex, selected after an extensive evaluation, uses the loop 7–11 as the central part of the epitope. The generated complex allows the following conclusions: 1) the neutralizing capacity of BCF2 toward the venom of C. noxius might rather be caused by the high venom concentration and toxicity of Cn2 than by a broad specificity, 2) the region involved in the binding of Cn2 to the Na+ channel, should overlap with the employed epitope region, and 3) contact residues SerL91, AsnL92, LeuH50, AspH56, TyrH95, and TyrH98 of BCF2 are candidates for mutations to broaden its specificity. Proteins 1999;37:130–143. © 1999 Wiley-Liss, Inc." @default.
- W4317676017 created "2023-01-22" @default.
- W4317676017 creator A5013948305 @default.
- W4317676017 creator A5032759906 @default.
- W4317676017 creator A5062608915 @default.
- W4317676017 creator A5071436463 @default.
- W4317676017 creator A5075427093 @default.
- W4317676017 creator A5088365197 @default.
- W4317676017 date "1999-10-01" @default.
- W4317676017 modified "2023-10-01" @default.
- W4317676017 title "Antibody BCF2 against scorpion toxin cn2 fromCentruroides noxius hoffmann: Primary structure and three-dimensional model as free fv fragment and complexed with its antigen" @default.
- W4317676017 cites W1490054248 @default.
- W4317676017 cites W1517161569 @default.
- W4317676017 cites W153015601 @default.
- W4317676017 cites W1566581292 @default.
- W4317676017 cites W1608855442 @default.
- W4317676017 cites W1719160721 @default.
- W4317676017 cites W1778948802 @default.
- W4317676017 cites W1866772093 @default.
- W4317676017 cites W1964463823 @default.
- W4317676017 cites W1966041739 @default.
- W4317676017 cites W1967210387 @default.
- W4317676017 cites W1969398164 @default.
- W4317676017 cites W1969407832 @default.
- W4317676017 cites W1974166931 @default.
- W4317676017 cites W1975914851 @default.
- W4317676017 cites W1977385571 @default.
- W4317676017 cites W1982679430 @default.
- W4317676017 cites W1984851850 @default.
- W4317676017 cites W1987958081 @default.
- W4317676017 cites W1989193897 @default.
- W4317676017 cites W1989670701 @default.
- W4317676017 cites W1991323330 @default.
- W4317676017 cites W1999271199 @default.
- W4317676017 cites W2000383223 @default.
- W4317676017 cites W2001807393 @default.
- W4317676017 cites W2002590736 @default.
- W4317676017 cites W2005481172 @default.
- W4317676017 cites W2006404858 @default.
- W4317676017 cites W2013083986 @default.
- W4317676017 cites W2020822614 @default.
- W4317676017 cites W2021411698 @default.
- W4317676017 cites W2023003128 @default.
- W4317676017 cites W2024319193 @default.
- W4317676017 cites W2026102463 @default.
- W4317676017 cites W2027584677 @default.
- W4317676017 cites W2031772330 @default.
- W4317676017 cites W2034314076 @default.
- W4317676017 cites W2041350126 @default.
- W4317676017 cites W2043001568 @default.
- W4317676017 cites W2045188132 @default.
- W4317676017 cites W2051628563 @default.
- W4317676017 cites W2053468356 @default.
- W4317676017 cites W2057484149 @default.
- W4317676017 cites W2058493142 @default.
- W4317676017 cites W2060289095 @default.
- W4317676017 cites W2065232574 @default.
- W4317676017 cites W2065335184 @default.
- W4317676017 cites W2070146818 @default.
- W4317676017 cites W2070205845 @default.
- W4317676017 cites W2071271247 @default.
- W4317676017 cites W2074534136 @default.
- W4317676017 cites W2076584494 @default.
- W4317676017 cites W2081142452 @default.
- W4317676017 cites W2082006283 @default.
- W4317676017 cites W2082545210 @default.
- W4317676017 cites W2083445427 @default.
- W4317676017 cites W2091563009 @default.
- W4317676017 cites W2093062941 @default.
- W4317676017 cites W2106315897 @default.
- W4317676017 cites W2107202459 @default.
- W4317676017 cites W2110036226 @default.
- W4317676017 cites W2112194058 @default.
- W4317676017 cites W2124448263 @default.
- W4317676017 cites W2128105883 @default.
- W4317676017 cites W2143467722 @default.
- W4317676017 cites W2144845679 @default.
- W4317676017 cites W2153731681 @default.
- W4317676017 cites W2157767795 @default.
- W4317676017 cites W2167795220 @default.
- W4317676017 cites W4302395856 @default.
- W4317676017 doi "https://doi.org/10.1002/(sici)1097-0134(19991001)37:1<130::aid-prot13>3.0.co;2-s" @default.
- W4317676017 hasPublicationYear "1999" @default.
- W4317676017 type Work @default.
- W4317676017 citedByCount "16" @default.
- W4317676017 countsByYear W43176760172012 @default.
- W4317676017 countsByYear W43176760172013 @default.
- W4317676017 countsByYear W43176760172014 @default.
- W4317676017 countsByYear W43176760172016 @default.
- W4317676017 countsByYear W43176760172017 @default.
- W4317676017 countsByYear W43176760172023 @default.
- W4317676017 crossrefType "journal-article" @default.
- W4317676017 hasAuthorship W4317676017A5013948305 @default.
- W4317676017 hasAuthorship W4317676017A5032759906 @default.
- W4317676017 hasAuthorship W4317676017A5062608915 @default.
- W4317676017 hasAuthorship W4317676017A5071436463 @default.
- W4317676017 hasAuthorship W4317676017A5075427093 @default.
- W4317676017 hasAuthorship W4317676017A5088365197 @default.