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- W4317708380 abstract "Abstract Potassium channels play essential roles in the regulation of male fertility. However, potassium channels mediating K + currents in human sperm (I KSper ) remain controversial. Besides SLO3, the SLO1 potassium channel is a potential candidate for human sperm KSper. This study intends to elucidate the function of SLO1 potassium channel during human sperm capacitation. Human sperm were treated with iberiotoxin (IbTX, a SLO1 specific inhibitor) and clofilium (SLO3 inhibitor) separately or simultaneously during in vitro capacitation. A computer‐assisted sperm analyzer was used to assess sperm motility. The sperm acrosome reaction (AR) was analyzed using fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin staining. Sperm protein tyrosine phosphorylation was studied using western blotting. Intracellular Ca 2+ , K + , Cl − , and pH were analyzed using ion fluorescence probes. Independent inhibition with IbTX or clofilium decreased the sperm hyperactivation, AR, and protein tyrosine phosphorylation, and was accompanied by an increase in [K + ] i , [Cl − ] i , and pH i , but a decrease in [Ca 2+ ] i . Simultaneously inhibition with IbTX and clofilium lower sperm hyperactivation and AR more than independent inhibition. The increase in [K + ] i , [Cl − ] i , and pH i , and the decrease in [Ca 2+ ] i were more pronounced. This study suggested that the SLO1 potassium channel may have synergic roles with SLO3 during human sperm capacitation." @default.
- W4317708380 created "2023-01-23" @default.
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- W4317708380 date "2023-01-22" @default.
- W4317708380 modified "2023-09-26" @default.
- W4317708380 title "Iberiotoxin and clofilium regulate hyperactivation, acrosome reaction, and ion homeostasis synergistically during human sperm capacitation" @default.
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- W4317708380 doi "https://doi.org/10.1002/mrd.23671" @default.
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