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• W4317733031 abstract "Identifying the cell types using unsupervised methods is essential for scRNA-seq research. However, conventional similarity measures introduce challenges to single-cell data clustering because of the high dimensional, high noise, and high dropout.We proposed a clustering method for small ScRNA-seq data based on Subspace and Weighted Distance (SSWD), which follows the assumption that the sets of gene subspace composed of similar density-distributing genes can better distinguish cell groups. To accurately capture the intrinsic relationship among cells or genes, a new distance metric that combines Euclidean and Pearson distance through a weighting strategy was proposed. The relative Calinski-Harabasz (CH) index was used to estimate the cluster numbers instead of the CH index because it is comparable across degrees of freedom.We compared SSWD with seven prevailing methods on eight publicly scRNA-seq datasets. The experimental results show that the SSWD has better clustering accuracy and the partitioning ability of cell groups. SSWD can be downloaded at https://github.com/ningzilan/SSWD." @default.
• W4317733031 created "2023-01-23" @default.
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• W4317733031 date "2023-01-23" @default.
• W4317733031 modified "2023-10-01" @default.
• W4317733031 title "A clustering method for small scRNA-seq data based on subspace and weighted distance" @default.
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• W4317733031 doi "https://doi.org/10.7717/peerj.14706" @default.
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