Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317743302> ?p ?o ?g. }
- W4317743302 endingPage "25" @default.
- W4317743302 startingPage "1" @default.
- W4317743302 abstract "The macroscale production of mussel foot proteins (Mfps) in the expression system has not succeeded to date. The principal reasons for this are low levels of expression and yield of Mfps, lack of post-translational modifications (PTMs), and immunological toxic effects on the host system. Identification of post-translational modification sites, suitable expression hosts, and immunological responses through an experimental approach is very costly and time-consuming. However, in the present study, in silico post-translation modification, antigenicity, allergenicity, and the immunological reaction of all available Mfps were characterized. Furthermore, all Mfps were codon optimized in three different expression systems to determine the best expression host. Finally, we performed the in-silico cloning of all codon-optimized Mfps in a suitable host (E. coli K12, pET28a(+) vector) and analyzed the secondary structure of mRNA and its structural stability. Among the 78 Mfps, six fps are considered potential allergenic proteins, six fps are considered non-allergenic proteins, and all other fps are probably allergenic. High antigenicity was observed in bacterial cells as compared to yeast and tumor cells. Nevertheless, the predicted expression of Mfps in a bacterial host is higher than in other expression hosts. Important to note that all Mfps showed significant immunological activity in the human system, and we concluded that these antigenic, allergenic, and immunological properties are directly correlated with their amino acid composition. The study's major goal is to provide a comprehensive understanding of Mfps and aid in the future genetic engineering and expression of Mfps and its diverse applications in different fields.Communicated by Ramaswamy H. Sarma." @default.
- W4317743302 created "2023-01-23" @default.
- W4317743302 creator A5010831015 @default.
- W4317743302 creator A5057353857 @default.
- W4317743302 date "2023-01-23" @default.
- W4317743302 modified "2023-09-29" @default.
- W4317743302 title "Molecular cloning, expression, mRNA secondary structure and immunological characterization of mussel foot proteins (Mfps) (Mollusca: Bivalvia)" @default.
- W4317743302 cites W140952802 @default.
- W4317743302 cites W1554789346 @default.
- W4317743302 cites W1576619789 @default.
- W4317743302 cites W1585589485 @default.
- W4317743302 cites W1968758654 @default.
- W4317743302 cites W1969586302 @default.
- W4317743302 cites W1980067778 @default.
- W4317743302 cites W1983152536 @default.
- W4317743302 cites W1983972834 @default.
- W4317743302 cites W1986271212 @default.
- W4317743302 cites W1991542542 @default.
- W4317743302 cites W1992011929 @default.
- W4317743302 cites W1993970485 @default.
- W4317743302 cites W1998270097 @default.
- W4317743302 cites W2002642674 @default.
- W4317743302 cites W2003805630 @default.
- W4317743302 cites W2005162839 @default.
- W4317743302 cites W2005368968 @default.
- W4317743302 cites W2006970770 @default.
- W4317743302 cites W2018590530 @default.
- W4317743302 cites W2035240910 @default.
- W4317743302 cites W2037263319 @default.
- W4317743302 cites W2041979681 @default.
- W4317743302 cites W2042221969 @default.
- W4317743302 cites W2045393613 @default.
- W4317743302 cites W2053145607 @default.
- W4317743302 cites W2061836132 @default.
- W4317743302 cites W2067107344 @default.
- W4317743302 cites W2067869022 @default.
- W4317743302 cites W2068594901 @default.
- W4317743302 cites W2068655550 @default.
- W4317743302 cites W2068767857 @default.
- W4317743302 cites W2068955417 @default.
- W4317743302 cites W2069777522 @default.
- W4317743302 cites W2074861758 @default.
- W4317743302 cites W2075878132 @default.
- W4317743302 cites W2079114027 @default.
- W4317743302 cites W2083921861 @default.
- W4317743302 cites W2084905544 @default.
- W4317743302 cites W2086412511 @default.
- W4317743302 cites W2097300414 @default.
- W4317743302 cites W2102023232 @default.
- W4317743302 cites W2102367710 @default.
- W4317743302 cites W2111772603 @default.
- W4317743302 cites W2115766133 @default.
- W4317743302 cites W2118484233 @default.
- W4317743302 cites W2124052876 @default.
- W4317743302 cites W2125520334 @default.
- W4317743302 cites W2130702391 @default.
- W4317743302 cites W2132507479 @default.
- W4317743302 cites W2134126327 @default.
- W4317743302 cites W2141157874 @default.
- W4317743302 cites W2142523532 @default.
- W4317743302 cites W2144633240 @default.
- W4317743302 cites W2145970798 @default.
- W4317743302 cites W2151932306 @default.
- W4317743302 cites W2152051291 @default.
- W4317743302 cites W2155841258 @default.
- W4317743302 cites W2156971386 @default.
- W4317743302 cites W2159286305 @default.
- W4317743302 cites W2160225114 @default.
- W4317743302 cites W2161420671 @default.
- W4317743302 cites W2167045004 @default.
- W4317743302 cites W2167178280 @default.
- W4317743302 cites W2168036974 @default.
- W4317743302 cites W2171295581 @default.
- W4317743302 cites W2172096659 @default.
- W4317743302 cites W2269026190 @default.
- W4317743302 cites W2290371181 @default.
- W4317743302 cites W2326249713 @default.
- W4317743302 cites W2375602335 @default.
- W4317743302 cites W2396608270 @default.
- W4317743302 cites W2531651920 @default.
- W4317743302 cites W2581955873 @default.
- W4317743302 cites W2622871172 @default.
- W4317743302 cites W2748834817 @default.
- W4317743302 cites W2753894792 @default.
- W4317743302 cites W2824150873 @default.
- W4317743302 cites W2898850852 @default.
- W4317743302 cites W2898965096 @default.
- W4317743302 cites W2909455948 @default.
- W4317743302 cites W2922503279 @default.
- W4317743302 cites W2961559963 @default.
- W4317743302 cites W3001179882 @default.
- W4317743302 cites W3003822175 @default.
- W4317743302 cites W3006307427 @default.
- W4317743302 cites W3011895364 @default.
- W4317743302 cites W3022685474 @default.
- W4317743302 cites W3023755923 @default.
- W4317743302 cites W3033263332 @default.
- W4317743302 cites W3172221677 @default.