FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317743574> ?p ?o ?g. }

• W4317743574 abstract "The correct establishment of DNA methylation patterns during mouse early development is essential for cell fate specification. However, the molecular targets as well as the mechanisms that determine the specificity of the de novo methylation machinery during differentiation are not completely elucidated. Here we show that the DNMT3B-dependent DNA methylation of key developmental regulatory regions at epiblast-like cells (EpiLCs) provides an epigenetic priming that ensures flawless commitment at later stages. Using in vitro stem cell differentiation and loss of function experiments combined with high-throughput genome-wide bisulfite-, bulk-, and single cell RNA-sequencing we dissected the specific role of DNMT3B in cell fate. We identify DNMT3B-dependent regulatory elements on the genome which, in Dnmt3b knockout (3BKO), impair the differentiation into meso-endodermal (ME) progenitors and redirect EpiLCs towards the neuro-ectodermal lineages. Moreover, ectopic expression of DNMT3B in 3BKO re-establishes the DNA methylation of the master regulator Sox2 super-enhancer, downmodulates its expression, and restores the expression of ME markers. Taken together, our data reveal that DNMT3B-dependent methylation at the epiblast stage is essential for the priming of the meso-endodermal lineages and provide functional characterization of the de novo DNMTs during EpiLCs lineage determination." @default.
• W4317743574 created "2023-01-23" @default.
• W4317743574 creator A5002897632 @default.
• W4317743574 creator A5003294860 @default.
• W4317743574 creator A5011049658 @default.
• W4317743574 creator A5012460681 @default.
• W4317743574 creator A5020931913 @default.
• W4317743574 creator A5022467253 @default.
• W4317743574 creator A5027697748 @default.
• W4317743574 creator A5031087396 @default.
• W4317743574 creator A5038395336 @default.
• W4317743574 creator A5046648782 @default.
• W4317743574 creator A5048479700 @default.
• W4317743574 creator A5055792143 @default.
• W4317743574 creator A5065934732 @default.
• W4317743574 creator A5068535122 @default.
• W4317743574 creator A5087775644 @default.
• W4317743574 date "2023-01-23" @default.
• W4317743574 modified "2023-09-25" @default.
• W4317743574 title "DNMT3B supports meso-endoderm differentiation from mouse embryonic stem cells" @default.
• W4317743574 cites W1971578941 @default.
• W4317743574 cites W1977463456 @default.
• W4317743574 cites W1979594866 @default.
• W4317743574 cites W1984077980 @default.
• W4317743574 cites W1985102214 @default.
• W4317743574 cites W1986342614 @default.
• W4317743574 cites W1987557775 @default.
• W4317743574 cites W2009573598 @default.
• W4317743574 cites W2015493376 @default.
• W4317743574 cites W2019552331 @default.
• W4317743574 cites W2045067641 @default.
• W4317743574 cites W2045069150 @default.
• W4317743574 cites W2046241907 @default.
• W4317743574 cites W2053056178 @default.
• W4317743574 cites W2060446375 @default.
• W4317743574 cites W2062691001 @default.
• W4317743574 cites W2067056027 @default.
• W4317743574 cites W2068780842 @default.
• W4317743574 cites W2069043023 @default.
• W4317743574 cites W2088128367 @default.
• W4317743574 cites W2090260381 @default.
• W4317743574 cites W2092116145 @default.
• W4317743574 cites W2096391240 @default.
• W4317743574 cites W2102689239 @default.
• W4317743574 cites W2107368073 @default.
• W4317743574 cites W2107665951 @default.
• W4317743574 cites W2108234281 @default.
• W4317743574 cites W2108744961 @default.
• W4317743574 cites W2111041757 @default.
• W4317743574 cites W2114104545 @default.
• W4317743574 cites W2124933556 @default.
• W4317743574 cites W2131374955 @default.
• W4317743574 cites W2158789637 @default.
• W4317743574 cites W2169456326 @default.
• W4317743574 cites W2170551349 @default.
• W4317743574 cites W2171808845 @default.
• W4317743574 cites W2305364952 @default.
• W4317743574 cites W2406250479 @default.
• W4317743574 cites W2407888728 @default.
• W4317743574 cites W2589386142 @default.
• W4317743574 cites W2610903912 @default.
• W4317743574 cites W2747297590 @default.
• W4317743574 cites W2766643978 @default.
• W4317743574 cites W2774946861 @default.
• W4317743574 cites W2782454362 @default.
• W4317743574 cites W2799520277 @default.
• W4317743574 cites W2916020270 @default.
• W4317743574 cites W2945883683 @default.
• W4317743574 cites W2964849163 @default.
• W4317743574 cites W2976747241 @default.
• W4317743574 cites W2992957227 @default.
• W4317743574 cites W2993308551 @default.
• W4317743574 cites W3009913140 @default.
• W4317743574 cites W3031159512 @default.
• W4317743574 cites W3036426980 @default.
• W4317743574 cites W3037673772 @default.
• W4317743574 cites W3038200747 @default.
• W4317743574 cites W3045805366 @default.
• W4317743574 cites W3045910546 @default.
• W4317743574 cites W3092446162 @default.
• W4317743574 cites W3128359329 @default.
• W4317743574 cites W3141751158 @default.
• W4317743574 cites W3164692211 @default.
• W4317743574 cites W4200591225 @default.
• W4317743574 cites W4254218046 @default.
• W4317743574 doi "https://doi.org/10.1038/s41467-023-35938-x" @default.
• W4317743574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36690616" @default.
• W4317743574 hasPublicationYear "2023" @default.
• W4317743574 type Work @default.
• W4317743574 citedByCount "2" @default.
• W4317743574 countsByYear W43177435742023 @default.
• W4317743574 crossrefType "journal-article" @default.
• W4317743574 hasAuthorship W4317743574A5002897632 @default.
• W4317743574 hasAuthorship W4317743574A5003294860 @default.
• W4317743574 hasAuthorship W4317743574A5011049658 @default.
• W4317743574 hasAuthorship W4317743574A5012460681 @default.
• W4317743574 hasAuthorship W4317743574A5020931913 @default.
• W4317743574 hasAuthorship W4317743574A5022467253 @default.
• W4317743574 hasAuthorship W4317743574A5027697748 @default.
• W4317743574 hasAuthorship W4317743574A5031087396 @default.

• next