Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317751706> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W4317751706 endingPage "1031" @default.
- W4317751706 startingPage "1025" @default.
- W4317751706 abstract "Abstract BACKGROUND Acetyl phosphate/acetate kinase system is a cost‐saving and efficient ATP regeneration system. However, it suffers from accumulation of salts during catalytic reactions. It inhibits the activity of acetate kinase (ACK), a crucial enzyme of this process, and hence decreases the rate of product accumulation and yield of CTP. RESULTS A new halotolerant ACK enzyme based ATP regeneration platform was established in this research by applying the concept of rational design to optimize its building blocks. Selection of residues for rational design followed several rules. First, the less conserved residue positions on the protein surface were considered. Second, avoiding significant residue interactions, suchas salt bridges. Third, following the feature of nature occurring halotolerant enzymes, the basic and neutral surface residues were replaced with acidic ones. Our mutants represent high catalytic activities and high IC50 value (inhibit activity by 50%) at high salt concentrations. Also, when the halotolerant ACK was applied to catalyze CTP production, the maximum titer was 213.5 ± 1.6 mM (which is 12.3‐fold higher than that of the traditional process), the productivity was 13.3 ± 0.1 mM L −1 h −1 , and the molar yield to 5′‐cytidylate monophosphate (CMP) and utilization efficiency of energy were 92.4% and 72.7%, respectively. CONCLUSION This strategy can be applied to modify other enzymes in the industry and gives insight into resolving salt accumulation during biocatalytic processes. In addition, the halotolerant Adenosine Triphosphate (ATP) regeneration system built in this work can be applied to the synthesis of several other high energy compounds. © 2023 Society of Chemical Industry (SCI)." @default.
- W4317751706 created "2023-01-23" @default.
- W4317751706 creator A5012227166 @default.
- W4317751706 creator A5030382963 @default.
- W4317751706 creator A5040494980 @default.
- W4317751706 creator A5043819331 @default.
- W4317751706 creator A5056036725 @default.
- W4317751706 creator A5071032933 @default.
- W4317751706 creator A5073501391 @default.
- W4317751706 creator A5077169310 @default.
- W4317751706 creator A5090767771 @default.
- W4317751706 date "2023-02-08" @default.
- W4317751706 modified "2023-09-26" @default.
- W4317751706 title "Rational design of a novel halotolerant <scp>ATP</scp> regeneration system for biocatalytic <scp>CTP</scp> production" @default.
- W4317751706 cites W1965322645 @default.
- W4317751706 cites W1988031310 @default.
- W4317751706 cites W1989036886 @default.
- W4317751706 cites W2000575260 @default.
- W4317751706 cites W2001042907 @default.
- W4317751706 cites W2003325276 @default.
- W4317751706 cites W2020542590 @default.
- W4317751706 cites W2034346905 @default.
- W4317751706 cites W2034401008 @default.
- W4317751706 cites W2047399603 @default.
- W4317751706 cites W2069866688 @default.
- W4317751706 cites W2077060980 @default.
- W4317751706 cites W2084261089 @default.
- W4317751706 cites W2084734478 @default.
- W4317751706 cites W2087046083 @default.
- W4317751706 cites W2094588780 @default.
- W4317751706 cites W2094931921 @default.
- W4317751706 cites W2098763126 @default.
- W4317751706 cites W2118233996 @default.
- W4317751706 cites W2137015675 @default.
- W4317751706 cites W2141272708 @default.
- W4317751706 cites W2151171638 @default.
- W4317751706 cites W2263915426 @default.
- W4317751706 cites W2531270313 @default.
- W4317751706 doi "https://doi.org/10.1002/jctb.7310" @default.
- W4317751706 hasPublicationYear "2023" @default.
- W4317751706 type Work @default.
- W4317751706 citedByCount "0" @default.
- W4317751706 crossrefType "journal-article" @default.
- W4317751706 hasAuthorship W4317751706A5012227166 @default.
- W4317751706 hasAuthorship W4317751706A5030382963 @default.
- W4317751706 hasAuthorship W4317751706A5040494980 @default.
- W4317751706 hasAuthorship W4317751706A5043819331 @default.
- W4317751706 hasAuthorship W4317751706A5056036725 @default.
- W4317751706 hasAuthorship W4317751706A5071032933 @default.
- W4317751706 hasAuthorship W4317751706A5073501391 @default.
- W4317751706 hasAuthorship W4317751706A5077169310 @default.
- W4317751706 hasAuthorship W4317751706A5090767771 @default.
- W4317751706 hasConcept C129513315 @default.
- W4317751706 hasConcept C181199279 @default.
- W4317751706 hasConcept C185592680 @default.
- W4317751706 hasConcept C18903297 @default.
- W4317751706 hasConcept C2779564974 @default.
- W4317751706 hasConcept C30992042 @default.
- W4317751706 hasConcept C53105671 @default.
- W4317751706 hasConcept C54355233 @default.
- W4317751706 hasConcept C55493867 @default.
- W4317751706 hasConcept C86803240 @default.
- W4317751706 hasConceptScore W4317751706C129513315 @default.
- W4317751706 hasConceptScore W4317751706C181199279 @default.
- W4317751706 hasConceptScore W4317751706C185592680 @default.
- W4317751706 hasConceptScore W4317751706C18903297 @default.
- W4317751706 hasConceptScore W4317751706C2779564974 @default.
- W4317751706 hasConceptScore W4317751706C30992042 @default.
- W4317751706 hasConceptScore W4317751706C53105671 @default.
- W4317751706 hasConceptScore W4317751706C54355233 @default.
- W4317751706 hasConceptScore W4317751706C55493867 @default.
- W4317751706 hasConceptScore W4317751706C86803240 @default.
- W4317751706 hasFunder F4320321001 @default.
- W4317751706 hasFunder F4320327518 @default.
- W4317751706 hasFunder F4320335577 @default.
- W4317751706 hasFunder F4320335777 @default.
- W4317751706 hasIssue "4" @default.
- W4317751706 hasLocation W43177517061 @default.
- W4317751706 hasOpenAccess W4317751706 @default.
- W4317751706 hasPrimaryLocation W43177517061 @default.
- W4317751706 hasRelatedWork W1850986032 @default.
- W4317751706 hasRelatedWork W1968721630 @default.
- W4317751706 hasRelatedWork W1998184313 @default.
- W4317751706 hasRelatedWork W2016552231 @default.
- W4317751706 hasRelatedWork W2019529003 @default.
- W4317751706 hasRelatedWork W2029732200 @default.
- W4317751706 hasRelatedWork W2065079376 @default.
- W4317751706 hasRelatedWork W2080203695 @default.
- W4317751706 hasRelatedWork W2084240653 @default.
- W4317751706 hasRelatedWork W4242974861 @default.
- W4317751706 hasVolume "98" @default.
- W4317751706 isParatext "false" @default.
- W4317751706 isRetracted "false" @default.
- W4317751706 workType "article" @default.